Fish-mediated nutrient flows from macroalgae habitats to coral reefs in the Red Sea.

Macroalgae canopies are common in tropical coastlines, and can be feeding grounds for coral reef fishes. We investigated whether fish transfer algal material from Sargassum-dominated macroalgae habitats to coral reefs by collecting gut contents of two herbivorous fish species (Naso elegans and N. unicornis) from coral reefs in the central Red Sea. On inshore reefs close to macroalgae canopies, Sargassum accounted for up to 41% of these species' gut contents while almost no Sargassum was found in the stomachs of fish on offshore reefs farther from macroalgae canopies. Using consumption and excretion rates from literature, we estimate that these fish consume up to 6.0 mmol C/m2 reef/day and excrete up to 10.8 µmol N/m2 reef/day and 1.0 µmol P/m2 reef/day across inshore reefs as a result of Sargassum consumption. Examining fish-mediated connections between habitats illuminates the role of fish as a vector of nutrition to nutrient-poor coral reefs.

Monitoring coastal water flow dynamics using sub-daily high-resolution SkySat satellite and UAV-based imagery.

Sub-daily tracking of dynamic features and events using high spatial resolution satellite imagery has only recently become possible, with advanced observational capabilities now available through tasking of satellite constellations. Here, we provide a first of its kind demonstration of using sub-daily 0.50 m resolution SkySat imagery to track coastal water flows, combining these data with object-based detection and a machine-learning approach to map the extent and concentration of two dye plumes. Coincident high-frequency unmanned aerial vehicle (UAV) imagery was also employed for quantitative modeling of dye concentration and evaluation of the sub-daily satellite-based dye tracking. Our results show that sub-daily SkySat imagery can track dye plume extent with low omission (8.73-16.05%) and commission errors (0.32-2.77%) and model dye concentration (coefficient of determination = 0.73; root mean square error = 28.68 ppb) with the assistance of high-frequency UAV data. The results also demonstrate the capabilities of using UAV imagery for scaling between field data and satellite imagery for tracking coastal water flow dynamics. This research has implications for monitoring of water flows and nutrient or pollution exchange, and it also demonstrates the capabilities of higher temporal resolution satellite data for delivering further insights into dynamic processes of coastal systems.

Dye tracing and concentration mapping in coastal waters using unmanned aerial vehicles.

Coastal water flows facilitate important nutrient exchanges between mangroves, seagrasses and coral reefs. However, due to the complex nature of tidal interactions, their spatiotemporal development can be difficult to trace via traditional field instrumentations. Unmanned aerial vehicles (UAVs) serve as ideal platforms from which to capture such dynamic responses. Here, we provide a UAV-based approach for tracing coastal water flows using object-based detection of dye plume extent coupled with a regression approach for mapping dye concentration. From hovering UAV images and nine subsequent flight surveys covering the duration of an ebbing tide in the Red Sea, our results show that dye plume extent can be mapped with low omission and commission errors when assessed against manual delineations. Our results also demonstrated that the interaction term of two UAV-derived indices may be employed to accurately map dye concentration (coefficient of determination = 0.96, root mean square error = 7.78 ppb), providing insights into vertical and horizontal transportation and dilution of materials in the water column. We showcase the capabilities of high-frequency UAV-derived data and demonstrate how field-based dye concentration measurements can be integrated with UAV data for future studies of coastal water flow dynamics.

Localized effects of offshore aquaculture on water quality in a tropical sea.

Aquaculture production has increased steadily in many tropical countries over the past few decades, although impact assessments have been frequently neglected. We investigated the impacts of an offshore barramundi fish farm on water quality in the southern-central Red Sea, a traditionally understudied tropical, oligotrophic, and semi-enclosed basin. Inorganic nutrients, particulate matter, chlorophyll-a, and heterotrophic bacteria were measured periodically over 8 months around the farm. Water down-current from the farm had, on average, more heterotrophic bacteria and chlorophyll-a than up-current (11% and 34% higher, respectively). Ratios of dissolved inorganic nitrogen:phosphorus down-current from the farm were lower than ratios up-current (mean 9.8 vs 16.0, respectively). Phosphate, inorganic nitrogen, and particulate matter showed patterns of enrichment associated with the farm after a fish feeding event. Strategies such as feed optimization and considering hydrodynamics in site selection may improve water quality for future fish farms in Saudi Arabia and other tropical countries.

Effect of Salt on the Metabolism of 'Candidatus Accumulibacter' Clade I and II.

Saline wastewater is known to affect the performance of phosphate-accumulating organisms (PAOs) in enhanced biological phosphorus removal (EBPR) process. However, studies comparing the effect of salinity on different PAO clades are lacking. In this study, 'Candidatus Accumulibacter phosphatis' Clade I and II (hereafter referred to as PAOI and PAOII) were highly enriched (∼90% in relative abundance as determined by quantitative FISH) in the form of granules in two sequencing batch reactors. Anaerobic and aerobic batch experiments were conducted to evaluate the effect of salinity on the kinetics and stoichiometry of PAOI and PAOII. PAOI and PAOII communities showed different priority in using polyphosphate (poly-P) and glycogen to generate ATP in the anaerobic phase when exposed to salt, with PAOI depending more on intracellular poly-P degradation (e.g., the proportion of calculated ATP derived from poly-P increased by 5-6% at 0.256 mol/L NaCl or KCl) while PAOII on glycolysis of intracellularly stored glycogen (e.g., the proportion of calculated ATP derived from glycogen increased by 29-30% at 0.256 mol/L NaCl or KCl). In the aerobic phase, the loss of phosphate uptake capability was more pronounced in PAOII due to the higher energy cost to synthesize their larger glycogen pool compared to PAOI. For both PAOI and PAOII, aerobic conversion rates were more sensitive to salt than anaerobic conversion rates. Potassium (K+) and sodium (Na+) ions exhibited different effect regardless of the enriched PAO culture, suggesting that the composition of salt is an important factor to consider when studying the effect of salt on EBPR performance.

Adsorption and desorption of arsenate on sandy sediments from contaminated and uncontaminated saturated zones: Kinetic and equilibrium modeling.

Application of empirical models to adsorption of contaminants on natural heterogeneous sorbents is often challenging due to the uncertainty associated with fitting experimental data and determining adjustable parameters. Sediment samples from contaminated and uncontaminated portions of a study site in Maine, USA were collected and investigated for adsorption of arsenate [As(V)]. Two kinetic models were used to describe the results of single solute batch adsorption experiments. Piecewise linear regression of data linearized to fit pseudo-first order kinetic model resulted in two distinct rates and a cutoff time point of 14-19 h delineating the biphasic behavior of solute adsorption. During the initial rapid adsorption stage, an average of 60-80% of the total adsorption took place. Pseudo-second order kinetic models provided the best fit to the experimental data (R(2) > 0.99) and were capable of describing the adsorption over the entire range of experiments. Both Langmuir and Freundlich isotherms provided reasonable fits to the adsorption data at equilibrium. Langmuir-derived maximum adsorption capacity (St) of the studied sediments ranged between 29 and 97 mg/kg increasing from contaminated to uncontaminated sites. Solid phase As content of the sediments ranged from 3.8 to 10 mg/kg and the As/Fe ratios were highest in the amorphous phase. High-pH desorption experiments resulted in a greater percentage of solid phase As released into solution from experimentally-loaded sediments than from the unaltered samples suggesting that As(V) adsorption takes place on different reversible and irreversible surface sites.

Survey of high school students' perceptions about their iPod use, knowledge of hearing health, and need for education.

PURPOSE: High school students' knowledge about hearing health and their perceptions of how they use personal listening devices (PLDs) including iPods were surveyed to determine the need, content, and preferred format for educational outreach to them. METHOD: This study was a descriptive convenience survey of students at a California high school. An 83-item questionnaire was administered in December 2009 to assess students' demographics, knowledge of hearing health, perceived iPod use, and risk activities. RESULTS: The response rate was 56%. Most of the students perceived that they used iPods safely. However, responses indicated that many of the respondents could be at risk of injury to themselves or others if they became unaware of their surroundings while listening to iPods. Some students were knowledgeable about hearing health and safe iPod use, but most needed information about hearing loss and hearing conservation. CONCLUSION: Most of the students needed education (e.g., via health classes or the Internet) about hearing health, the warning signs of hearing loss, and how to prevent hearing loss. Resources for educational audiologists are provided.