ABSTRACT
This article has provided an overview of the current imaging techniques for evaluating head and neck cancer. Advantages, disadvantages, and complementary roles of CT and MRI have been described.
Subject(s)
Head and Neck Neoplasms/diagnosis , Magnetic Resonance Imaging , Tomography, X-Ray Computed , Biopsy, Needle/methods , Head and Neck Neoplasms/pathology , HumansABSTRACT
In this report, we describe the production and characterization of the first human-human hybridoma secreting antibody to HLA Class II determinants. The hybridoma (GMEC101), which has been stable in tissue culture for greater than 20 mo, secretes 10 to 50 micrograms/ml of IgM-kappa antibody. This antibody binds to a wide range of human cell lines, but not to the HLA-A,B,C, and DR-negative K562 cell line. Functionally, GMEC101 strongly inhibits a unidirectional mixed lymphocyte reaction (MLR) at the level of the stimulator cell. Neither the cellular ELISA binding nor the MLR inhibition is lost after a triple platelet absorption (which removes Class I but not Class II activity). Because the binding and MLR blocking show no correlation with the known DR or DQ specificities, we suggest that GMEC101 may be detecting a novel HLA Class II determinant.
