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1.
Anal Biochem ; 482: 16-21, 2015 Aug 01.
Article in English | MEDLINE | ID: mdl-25935259

ABSTRACT

Basement membrane collagens or derived fragments are measured in biological fluids such as blood and urine of patients and appear to be useful for diagnosis, prognostication, or treatment monitoring as proposed for endostatin, a fragment of collagen XVIII, or tumstatin, a fragment of collagen IV. Tetrastatin, the NC1 alpha 4 collagen IV domain, was previously reported to inhibit tumor growth and angiogenesis. The aim of this study was to develop and validate a method to measure tetrastatin concentrations in human fluids. We developed a competitive enzyme-linked immunosorbent assay (ELISA). It allowed measuring tetrastatin levels in human serum, bronchial aspiration and bronchoalveolar lavage fluids, and lung tissue extracts. The tetrastatin level was significantly higher in tumor tissues than in healthy lung tissues. Tetrastatin competitive ELISA could be useful to quantify tetrastatin in tissues and biological fluids for the diagnosis or prognostication of diseases in which basement membrane metabolism may be altered, especially tumor progression.


Subject(s)
Collagen Type IV/analysis , Collagen Type IV/blood , Enzyme-Linked Immunosorbent Assay/methods , Lung Neoplasms/blood , Lung Neoplasms/diagnosis , Lung/pathology , Adolescent , Adult , Aged , Aged, 80 and over , Bronchoalveolar Lavage Fluid/chemistry , Child , Female , Humans , Lung/chemistry , Lung Neoplasms/pathology , Male , Middle Aged , Protein Structure, Tertiary , Young Adult
2.
Anal Biochem ; 437(2): 111-7, 2013 Jun 15.
Article in English | MEDLINE | ID: mdl-23499763

ABSTRACT

Type XIX collagen is a minor collagen associated with basement membranes in vascular, neuronal, mesenchymal, and epithelial tissues. We demonstrated that the NC1, C-terminal, domain of collagen XIX inhibits the migration capacities of tumor cells and exerts a strong inhibition of tumor growth. Other basement membrane collagens or derived fragments were measured in biological fluids such as blood and urine of patients and appeared to be useful for diagnosis, prognosis, or treatment monitoring. The aim of this study was to develop and validate methods to measure collagen XIX and its fragments in human cell cultures, tissue extracts, and human biological fluids. For that purpose, we developed real-time PCR, Western blot, and competitive enzyme-linked immunosorbent assays. We demonstrated that the methods developed in this paper are specific for collagen XIX. We showed that it is expressed in human cell cultures, tissue extracts, and various biological fluids. These methods may be used in various human tissue extracts and biological fluids such as serum, amniotic fluid, cord blood, and many other fluids. Collagen XIX or its fragments could constitute new biomarkers for human diseases as well as for diagnosis and/or prognosis.


Subject(s)
Body Fluids/chemistry , Collagen/classification , Collagen/metabolism , Epithelial Cells/metabolism , Fibroblasts/metabolism , Tissue Extracts/chemistry , Cell Line , Collagen/chemistry , Enzyme-Linked Immunosorbent Assay , Epithelial Cells/chemistry , Fibroblasts/chemistry , Gene Expression Regulation/physiology , Humans , Osteosarcoma/chemistry , Osteosarcoma/metabolism
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