1.
Methods Mol Biol
; 667: 267-79, 2010.
Article
in English
| MEDLINE
| ID: mdl-20827540
ABSTRACT
RNA editing by A-to-I modification is a widespread mechanism in complex organisms that leads to the posttranscriptional alteration of protein coding as well as noncoding sequences. MiRNA transcripts have been recognized as a major target for RNA editing enzymes, and single-nucleotide changes through editing can impact the biogenesis of mature miRNAs, as well as the target specificity of the regulatory RNA. Bona fide A-to-I RNA editing events are validated experimentally through parallel analysis of genomic DNA and transcribed sequences of miRNA genes isolated from the same specimen through gene-specific amplification and sequencing of endogenous transcripts.