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Discovery of novel plant peptides as strong inhibitors of metalloproteinases.

Carrilho, Dina M; Duarte, Isabel C; Francisco, Rita; Ricardo, Cândido P P; Duque-Magalhães, Maria C.

Protein Pept Lett ; 16(5): 543-51, 2009.

Article in English | MEDLINE | ID: mdl-19442234

ABSTRACT

Five novel metalloproteinase protein inhibitors (MPIs) with molecular mass between 5.6 and 8.9 kDa and acid/neutral pI were detected in lupin seeds and exhibited strong inhibitory activities against thermolysin and/or gelatinase B. These novel peptides constitute not only the first MPIs described in plants but also the first plant peptides with inhibitory activity against a matrixin.

Subject(s)

Drug Discovery , Lupinus/chemistry , Metalloproteases/antagonists & inhibitors , Peptides/pharmacology , Plant Proteins/pharmacology , Protease Inhibitors/pharmacology , Animals , Bacteria/enzymology , Computational Biology , Databases, Protein , Electrophoresis, Gel, Two-Dimensional , Humans , Molecular Weight , Peptides/analysis , Peptides/chemistry , Plant Extracts/analysis , Plant Extracts/chemistry , Plant Extracts/pharmacology , Plant Proteins/analysis , Plant Proteins/chemistry , Protease Inhibitors/analysis , Protease Inhibitors/chemistry , Proteomics , Seeds/chemistry , Tandem Mass Spectrometry , Thermolysin/antagonists & inhibitors , Ultrafiltration

ABSTRACT

Subject(s)

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