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1.
Environ Res ; 82(1): 7-17, 2000 Jan.
Article in English | MEDLINE | ID: mdl-10677142

ABSTRACT

To determine the antioxidant effects of taurine on changes in memory and lipid peroxidation levels in brain caused by exposure to ozone, we carried out two experiments. In the first experiment, 150 rats were separated into three experimental blocks (young, mature, and old) with five groups each and received one of the following treatments: control, taurine, ozone, taurine before ozone, and taurine after ozone. Ozone exposure was 0.7-0.8 ppm for 4 h and taurine was administered ip at 43 mg/kg, after or before ozone exposure. Subsequently, rats were tested in passive avoidance conditioning. In the second experiment, samples from frontal cortex, hippocampus, striatum, and cerebellum were obtained from 60 rats (young and old), using the same treatments with 1 ppm ozone. Results show both an impairment in short-term and long-term memory with ozone and an improvement with taurine after ozone exposure, depending on age. In contrast to young rats, old rats showed peroxidation in all control groups and an improvement in memory with taurine. When taurine was applied before ozone, we found high peroxidation levels in the frontal cortex of old rats and the hippocampus of young rats; in the striatum, peroxidation caused by ozone was blocked when taurine was applied either before or after ozone exposure.


Subject(s)
Aging/drug effects , Antioxidants/pharmacology , Brain/drug effects , Lipid Peroxidation/drug effects , Memory/drug effects , Ozone/toxicity , Taurine/pharmacology , Animals , Avoidance Learning/drug effects , Brain/physiology , Cerebellum/drug effects , Hippocampus/drug effects , Male , Motor Activity/drug effects , Rats , Rats, Wistar
2.
Brain Res ; 744(1): 171-4, 1997 Jan 02.
Article in English | MEDLINE | ID: mdl-9030429

ABSTRACT

Recently, we have shown that rapid eye movement sleep deprivation (REM-SD) in animals with lesions of the nigro-striatal pathway facilitates turning behavior and such increase still occurred even in the presence of dopaminergic grafts. The objective of this work was to determine which DA receptors are preferentially involved. The results showed that the D2 receptor antagonist sulpiride decreases significantly turning behavior of lesioned animals, with no effect whatsoever of the D1 antagonist SCH 23390. When lesioned animals were REM sleep deprived, the D1 but not the D2 receptor antagonist prevented the increase of turning induced by REM-SD. This work suggests that the increase of post-synaptic supersensitivity induced by REM-SD in nigro-striatal lesioned animals is mediated by D1 receptors.


Subject(s)
Neostriatum/physiology , Receptors, Dopamine D1/physiology , Sleep Deprivation/physiology , Sleep, REM/physiology , Substantia Nigra/physiology , Animals , Benzazepines/pharmacology , Dopamine Antagonists/pharmacology , Dopamine D2 Receptor Antagonists , Locomotion/drug effects , Male , Motor Neurons/physiology , Neostriatum/cytology , Neostriatum/surgery , Rats , Rats, Wistar , Receptors, Dopamine D1/antagonists & inhibitors , Receptors, Dopamine D2/physiology , Rotation , Substantia Nigra/cytology , Substantia Nigra/surgery , Sulpiride/pharmacology
3.
Brain Res ; 729(2): 170-5, 1996 Aug 12.
Article in English | MEDLINE | ID: mdl-8876985

ABSTRACT

Since both REM sleep deprivation and unilateral 6-OHDA lesions induce supersensitivity of DA receptors, the purpose of this study was to determine whether the response of rats with such lesions would be modified by REM sleep deprivation. In addition, the effect of grafts of dissociated chromaffin cells was also tested. Rats with 6-OHDA lesions were subjected to 24 or 72 h of REM sleep deprivation and tested with various doses of apomorphine to determine turning behavior frequencies. At end of those experiments, the animals were transplanted with dissociated chromaffin cells and turning behavior was tested again. The results showed that REM sleep deprivation nearly doubled the turning behavior frequency, that chromaffin cell grafts decreased it, but that REM deprivation in grafted animals still seemed to produce an increase of post-synaptic supersensitivity independent of denervation. The results were discussed in terms of the possible relationship of sleep with Parkinson's disease through the DA system.


Subject(s)
Chromaffin Cells/transplantation , Corpus Striatum/drug effects , Oxidopamine/pharmacology , Sleep Deprivation , Sleep, REM/drug effects , Animals , Apomorphine/pharmacology , Behavior, Animal/drug effects , Cerebral Cortex/drug effects , Immunohistochemistry , Male , Rats , Rats, Wistar
4.
Brain Res ; 728(1): 37-46, 1996 Jul 22.
Article in English | MEDLINE | ID: mdl-8864295

ABSTRACT

Vasoactive intestinal peptide (VIP) has been shown to increase rapid eye movement (REM) sleep in normal and insomniac animals, while the administration of anti-VIP antibodies or an antagonist of VIP receptors decreases REM sleep. In addition, recently, it has been suggested that a VIP-like substance accumulates in the CSF during waking and that it may be involved in the production of the REM rebound normally seen following REM sleep deprivation. This evidence suggests that VIP may be important in modulating REM sleep in normal conditions and during REM sleep rebound. To determine whether VIP is involved in REM sleep homeostasis, VIP receptors of discrete brain areas was determined by autoradiography after 24 and 72 h of REM sleep deprivation (REM SD) by the water tank technique. Since this procedure has been suggested to produce some stress, an additional group adapted for 7 days to the sleep deprivation situation was tested. The results showed that REM SD produces an increase in the density of VIP receptors in several brainstem and forebrain structures at 24 h of REM SD and more so at 72 h of REM SD. Interestingly, results showed that habituation to the REM SD procedure decreases the density of VIP receptors in some areas of the brain of the REM sleep-deprived rats. The results are discussed in terms of the possibility that waking induces an increase of VIP receptors in several structures, which in turn are responsible for modulating REM sleep, but that stress contributes in part to VIP receptor changes.


Subject(s)
Brain Chemistry/physiology , Receptors, Vasoactive Intestinal Peptide/analysis , Sleep Deprivation/physiology , Sleep, REM/physiology , Animals , Autoradiography , Brain Stem/chemistry , Diencephalon/chemistry , Habituation, Psychophysiologic/physiology , Male , Prosencephalon/chemistry , Rats , Rats, Wistar
5.
Sleep ; 17(3): 231-5, 1994 Apr.
Article in English | MEDLINE | ID: mdl-7939122

ABSTRACT

Rapid eye movement (REM) sleep deprivation and auditory stimulation (ADS), separately, increase REM sleep in rats, cats and humans. The main goal of the present study was to test whether administration of ADS during REM sleep rebound has a synergistic effect on REM sleep elicitation. Male Wistar rats were implanted with standard sleep recording electrodes. Following the recovery period, animals were randomly assigned to the following conditions: undeprived (i.e. control) and 24, 48, 96 and 120 hours of REM sleep deprivation by the platform method. Undeprived and REM sleep-deprived animals were divided into two groups, with and without ADS. ADS was a "beep" of 80 dB and 2,000 Hz, lasting 20 msec every 10 seconds. This stimulus was applied for the first 4 hours of sleep recordings after deprivation. After that, animals were recorded for another 4 hours. In the undeprived situation, the group that received ADS increased REM sleep approximately 70% above the group that did not receive ADS, as has been reported previously (REM sleep without ADS: 38.1 +/- 13.84 vs. with ADS: 64.6 +/- 11.8, p < 0.005). No synergistic effect was observed between REM sleep deprivation and ADS for any REM sleep-deprivation schedule. This result may be explained as an increase in the excitability pattern of pontine neurons and/or changes in the cholinergic system due to REM sleep deprivation that could not be further increased by ADS.


Subject(s)
Acoustic Stimulation , Sleep Deprivation , Sleep, REM , Acetylcholine/physiology , Animals , Male , Rats , Rats, Wistar/physiology , Time Factors , Wakefulness
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