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1.
Retrovirology ; 13(1): 39, 2016 06 06.
Article in English | MEDLINE | ID: mdl-27267272

ABSTRACT

BACKGROUND: During the first trimester of pregnancy, HIV-1 in utero transmission is rare despite the permissivity of the placenta and the decidua (the uterine mucosa during pregnancy) to infection. In the decidua from the first trimester of pregnancy, macrophages (dMs) are the HIV-1 main target cells. Decidual natural killer (dNK) cells account for 70 % of decidual leukocytes. They display distinct phenotype and functions compared to peripheral NK cells. At the periphery, NK cells are involved in the control of HIV-1 infection. In this study, we investigate whether human decidual natural killer (dNK) cells control dM HIV-1 infection. RESULTS: Autologous cocultures of infected dMs with dNK cells reveal that dNK cells strongly inhibit dM HIV-1 infection. The addition of dNK cells to dMs at different times after infection suggests that the control occurs before the complete establishment of the infection. Double chamber cocultures show that cellular contacts are necessary for an optimal control of infection. Nevertheless, soluble factors secreted by dMs and dNK cells in double chamber cocultures partially inhibit dM HIV-1 infection, indicating that soluble factors have also a role in the control of infection. IFN-γ secretion is increased in infected and uninfected cocultures. We show that IFN-γ is involved in the control of dM HIV-1 infection by dNK cells. CONCLUSIONS: These results demonstrate that human dNK cells inhibit efficiently HIV-1 infection in dMs in vitro, and highlight the role of innate immune determinants in the control of HIV-1 transmission.


Subject(s)
Decidua/cytology , Decidua/immunology , HIV-1/physiology , Killer Cells, Natural/immunology , Macrophages/virology , Cells, Cultured , Coculture Techniques , Culture Media/chemistry , Decidua/chemistry , Female , HIV Infections/transmission , Humans , Infectious Disease Transmission, Vertical , Interferon-gamma/metabolism , Pregnancy , Pregnancy Trimester, First
2.
Mucosal Immunol ; 9(3): 634-46, 2016 05.
Article in English | MEDLINE | ID: mdl-26349662

ABSTRACT

Macrophages from the decidua basalis (dM), the main uterine mucosa during pregnancy, are weakly permissive to HIV-1 infection. Here, we investigated the mechanisms underlying this natural control. We show, by using freshly purified decidual macrophages and ex vivo human decidual explants, that the local decidual environment influences dM differentiation and naturally protects these cells from HIV-1 infection. Interferon (IFN)-γ, present in the decidual tissue, contributes to maintenance of the dM phenotype and restricts HIV-1 infection by mechanisms involving the cyclin-dependent kinase inhibitor p21Cip1/Waf1. We also found that activation of Toll-like receptors 7 and 8 expressed by dM reinforces the low permissivity of dM to HIV-1 by restricting viral replication and inducing secretion of cytokines in the decidual environment, including IFN-γ, that shape dM plasticity. A major challenge for HIV-1 eradication is to control infection of tissue-resident macrophages in the female reproductive tract. Our findings provide clues to the development of novel strategies to prevent HIV-1 macrophage infection.


Subject(s)
Decidua/immunology , HIV Infections/immunology , HIV-1/physiology , Macrophages/immunology , Cell Differentiation , Cells, Cultured , Cellular Microenvironment , Cyclin-Dependent Kinase Inhibitor p21/metabolism , Female , Humans , Immunity, Mucosal , Interferon-gamma/metabolism , Macrophages/virology , Toll-Like Receptor 7/metabolism , Toll-Like Receptor 8/metabolism , Virus Replication
3.
Circ Res ; 89(12): 1209-15, 2001 Dec 07.
Article in English | MEDLINE | ID: mdl-11739287

ABSTRACT

Ror alpha is an orphan nuclear receptor. In homozygous staggerer mutant mice (Ror alpha(sg/sg)), a deletion within the Ror alpha gene leads to an overexpression of inflammatory cytokines. Because inflammation and hypoxia are 2 key stimuli of ischemia-induced angiogenesis, we studied the role of Ror alpha in this setting. Ischemia was induced by ligation of the right femoral artery in C57BL/6 Ror alpha(+/+) and Ror alpha(sg/sg) mice. After 3 and 28 days, angiogenesis was evaluated by microangiography, measurement of capillary density using immunohistochemistry (anti-CD31), and measurement of blood flow by laser Doppler imaging. At day 3, angiographic score and blood flow were similar in Ror alpha(sg/sg) mice and in Ror alpha(+/+) littermates. Conversely, at day 28, Ror alpha(sg/sg) mice showed a significant 2-fold increase in angiographic score and a 3-fold increase in capillary density within the ischemic hindlimb compared with control. Functionally, this coincided with a significant rise in leg perfusion in Ror alpha(sg/sg) mice (0.83+/-0.05 for ischemic/nonischemic leg perfusion ratio) compared with Ror(+/+) mice (0.66+/-0.04, P<0.05). In addition, more extensive angiogenesis in Ror alpha(sg/sg) mice correlated with an increased expression of eNOS protein by 83+/-12% and 71+/-24% at 3 and 28 days, respectively (P<0.05), whereas the level of the antiangiogenic cytokine IL-12 was significantly reduced by 38+/-10% at day 28 (P<0.05). Conversely, no changes in VEGF expression were observed. Our study identifies for the first time a new role for Ror alpha as a potent negative regulator of ischemia-induced angiogenesis.


Subject(s)
Ischemia/metabolism , Neovascularization, Physiologic/physiology , Receptors, Cytoplasmic and Nuclear/deficiency , Receptors, Cytoplasmic and Nuclear/metabolism , Trans-Activators/deficiency , Trans-Activators/metabolism , Animals , Arterioles/cytology , Blood Flow Velocity/physiology , Blotting, Western , Capillaries/cytology , Endothelial Growth Factors/metabolism , Femoral Artery/physiology , Hindlimb/blood supply , Interleukin-12/metabolism , Laser-Doppler Flowmetry , Ligation , Lymphokines/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Neurologic Mutants , Microcirculation/physiology , Muscle, Skeletal/blood supply , Muscle, Skeletal/cytology , Muscle, Skeletal/metabolism , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Nitric Oxide Synthase Type III , Nuclear Receptor Subfamily 1, Group F, Member 1 , Platelet Endothelial Cell Adhesion Molecule-1/metabolism , RNA, Messenger/metabolism , Receptors, Cytoplasmic and Nuclear/genetics , Regional Blood Flow/physiology , Trans-Activators/genetics , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
4.
Arterioscler Thromb Vasc Biol ; 21(10): 1598-603, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11597932

ABSTRACT

This study investigated in vivo the putative angiogenic role of endothelin (ET)-1 in a model of ischemia-induced angiogenesis. Ischemia was produced by unilateral femoral artery occlusion in Wistar rats submitted to either chronic ET-1 infusion (2 nmol. kg(-1). min(-1)) or to a dual ET(A)/ET(B) receptor antagonist (bosentan, 100 mg. kg(-1). d(-1)) for 3 and 28 days. Arterial density was evaluated by microangiography and measurement of capillary and arteriolar density in hindlimb muscles. ET-1 infusion had no effect on ischemia-induced angiogenesis and was associated with a slight decrease in vascular endothelial growth factor (VEGF) content measured by Western blot analysis. Conversely, bosentan induced a marked increase in vessel density at 3 and 28 days (1.4-fold and 1.7-fold, respectively, compared with no treatment; P<0.05), which was associated with an increase in VEGF and endothelial NO synthase levels in ischemic legs (by 31+/-8% and 45+/-23%, respectively, at 3 days and by 65+/-13% and 55+/-15%, respectively, at 28 days; P<0.05 versus nontreated rats). At day 28, the proangiogenic effect of bosentan was abolished when NO synthesis inhibitor N(G)-nitro-L-arginine methyl ester (10 mg. kg(-1). d(-1)) or VEGF-neutralizing antibody (2.5 micro/kg twice a week) were coadministered with bosentan. Those results provide the first evidence of an early and sustained proangiogenic effect of endothelin antagonism associated with an upregulation of VEGF and endothelial NO synthase in vivo.


Subject(s)
Endothelial Growth Factors/metabolism , Endothelin Receptor Antagonists , Ischemia/blood , Ischemia/metabolism , Lymphokines/metabolism , Neovascularization, Physiologic , Nitric Oxide/metabolism , Animals , Antibodies/pharmacology , Bosentan , Capillaries/diagnostic imaging , Capillaries/physiology , Endothelial Growth Factors/immunology , Endothelin-1/pharmacology , Enzyme Inhibitors/pharmacology , Hindlimb/blood supply , Hindlimb/diagnostic imaging , Ischemia/diagnostic imaging , Lymphokines/immunology , Male , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase/biosynthesis , Nitric Oxide Synthase Type III , Radiography , Rats , Rats, Wistar , Receptor, Endothelin A , Signal Transduction , Sulfonamides/pharmacology , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
5.
Circ Res ; 89(8): 678-83, 2001 Oct 12.
Article in English | MEDLINE | ID: mdl-11597990

ABSTRACT

Recent studies have suggested a proangiogenic effect of angiotensin-converting enzyme (ACE) inhibition. We hypothesized that such a proangiogenic effect of ACE inhibition may be mediated, in part, by bradykinin (BK) B(2)-receptor pathway. This study therefore examined the neovascularization induced by ACE inhibitor treatment in B(2) receptor-deficient mice (B(2)(-/-)) in a model of surgically induced hindlimb ischemia. After artery femoral occlusion, wild-type and B(2)(-/-) mice were treated with or without ACE inhibitor (perindopril, 3 mg/kg/d) for 28 days. Angiogenesis was then quantitated by microangiography, capillary density measurement, and laser Doppler perfusion imaging. The protein levels of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) were determined by Western blot. In wild-type animals, vessel density and capillary number in the ischemic leg were raised by 1.8- and 1.4-fold, respectively, in mice treated with ACE inhibitor when compared with the nontreated animals (P<0.01). This corresponded to an improved ischemic/nonischemic leg perfusion ratio by 1.5-fold in ACE inhibitor-treated animals when compared with the untreated ones (0.87+/-0.07 versus 0.59+/-0.05, respectively, P<0.01). Activation of the angiogenic process was also associated with a 1.7-fold increase in tissue eNOS protein level in mice treated with ACE inhibitor (P<0.05 versus control) but not with changes in VEGF protein level. Conversely, ACE inhibition did not affect vessel density, blood flow, and eNOS protein level in ischemic hindlimb of B(2)(-/-) mice. Therefore, proangiogenic effect of ACE inhibition is mediated by B(2)-receptor signaling and was associated with upregulation of eNOS content, independently of VEGF expression.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/pharmacology , Ischemia/physiopathology , Neovascularization, Physiologic/drug effects , Receptors, Bradykinin/metabolism , Signal Transduction/drug effects , Animals , Antihypertensive Agents/pharmacology , Blood Flow Velocity/drug effects , Blood Pressure/drug effects , Capillaries/drug effects , Capillaries/pathology , Cell Count , Cyclic GMP/metabolism , Disease Models, Animal , Endothelial Growth Factors/metabolism , Femoral Artery/physiopathology , Hindlimb/blood supply , Hindlimb/drug effects , Hindlimb/physiopathology , Lymphokines/metabolism , Male , Mice , Mice, Knockout , Nitric Oxide/metabolism , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Nitric Oxide Synthase Type III , Receptor, Bradykinin B2 , Receptors, Bradykinin/deficiency , Receptors, Bradykinin/genetics , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
6.
Circ Res ; 89(3): 259-64, 2001 Aug 03.
Article in English | MEDLINE | ID: mdl-11485976

ABSTRACT

We have previously shown that deficiency in the anti-inflammatory cytokine interleukin-10 (IL-10) is responsible for enhanced angiogenesis after hindlimb ischemia. This study examined the putative involvement of matrix metalloproteinase (MMP) activation in this process. Ischemia was produced by artery femoral occlusion in both C57BL6 IL-10(+/+) and IL-10(-/-) mice. Angiographic vessel density and laser Doppler perfusion data at day 28 showed significant improvement in ischemic/nonischemic leg ratio by, respectively, 1.8-fold and 1.4-fold in IL-10(-/-) mice compared with IL-10(+/+) mice. This was associated with an increase in vascular endothelial growth factor (VEGF) protein content in the ischemic hindlimb. Three days after ischemia, gelatin zymography showed a significant increase in both pro- and active forms of MMP-2 and MMP-9 in ischemic hindlimbs of IL-10(-/-) mice compared with IL-10(+/+) mice (P<0.01). This increase in MMP activity in IL-10(-/-) mice was completely inhibited by treatment with BB-94 (5 mg/kg IP), a specific MMP inhibitor. Furthermore, increases in both vessel density and blood perfusion indexes at day 28 in IL-10(-/-) mice were abolished after treatment with BB-94 (0.78+/-0.06 versus 1.17+/-0.09 and 0.62+/-0.02 versus 0.88+/-0.04, for vessel density and blood perfusion ratio, respectively, in IL-10(-/-) mice treated with BB-94 versus untreated IL-10(-/-) mice, P<0.05). In contrast, BB-94 treatment did not affect the rise in VEGF protein content. These findings in IL-10(-/-) mice underscore the critical role of MMP activation, in a context of increased VEGF expression, in promoting ischemia-induced angiogenesis.


Subject(s)
Interleukin-10/metabolism , Ischemia/physiopathology , Matrix Metalloproteinases/metabolism , Neovascularization, Pathologic/physiopathology , Angiography , Animals , Blood Flow Velocity , Capillaries/cytology , Capillaries/metabolism , Disease Models, Animal , Endothelial Growth Factors/metabolism , Enzyme Activation/drug effects , Femoral Artery/diagnostic imaging , Femoral Artery/physiology , Fibroblast Growth Factor 2/metabolism , Hindlimb/blood supply , Hindlimb/diagnostic imaging , Hindlimb/physiopathology , Interleukin-10/deficiency , Interleukin-10/pharmacology , Ischemia/complications , Laser-Doppler Flowmetry , Lymphokines/metabolism , Matrix Metalloproteinase Inhibitors , Mice , Mice, Inbred C57BL , Mice, Knockout , Neovascularization, Pathologic/etiology , Neovascularization, Pathologic/prevention & control , Phenylalanine/analogs & derivatives , Phenylalanine/pharmacology , Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis , Protease Inhibitors/pharmacology , Thiophenes/pharmacology , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
7.
Clin Exp Pharmacol Physiol ; 28(5-6): 371-5, 2001.
Article in English | MEDLINE | ID: mdl-11380508

ABSTRACT

1. In normotensive rats, removal of carotid artery endothelium results in an acute increase in diameter. This finding, observed in young animals, has not been investigated in old animals. The present study was undertaken to assess the contribution of endothelial function in the regulation of arterial stiffness in aged rats. 2. In normotensive female WAG/Rij rats, isobaric (100 mmHg transmural pressure) carotid diameter was measured in vitro in situ, using a previously described arterial preparation associated with a high-resolution echotracking technique allowing non-invasive diameter measurements under baseline conditions, after removal of the endothelium and after total relaxation of vascular smooth muscle by potassium cyanide. Histomorphometry of the carotid wall was studied after pressure fixation (100 mmHg) of the arteries. 3. Compared with younger animals (10 months), older animals (30 months) had the same baseline carotid isobaric diameter but significantly higher values of wall thickness and collagen content. In older animals, whereas total relaxation by potassium cyanide was associated with a slight but significant increase of isobaric diameter, no increase was observed after endothelium removal. 4. The results of the presnt study provide evidence that, in old normotensive rats, endothelium-dependent increases in isobaric carotid diameter are blunted. This endothelium alteration may contribute to the age-dependent increase in isobaric carotid stiffness observed in old rats.


Subject(s)
Aging/physiology , Carotid Artery, Common/anatomy & histology , Carotid Artery, Common/physiology , Endothelium, Vascular/physiology , Animals , Blood Pressure/physiology , Body Weight/physiology , Female , Poisons/pharmacology , Potassium Cyanide/pharmacology , Rats , Rats, Inbred Strains
8.
Am J Hypertens ; 14(1): 7-13, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11206683

ABSTRACT

The development of hypertension is accompanied by rarefaction of arterioles and capillaries in both animal models and humans. Although many studies have examined the effects of antihypertensive therapies on hemodynamics, cardiac hypertrophy, and large vessel structure, the question of whether changes in microvascular density induced by hypertension can be restored by pharmacologic treatment has yet to be answered. We report a series of experiments performed in rats with renovascular hypertension induced by unilateral nephrectomy and renal artery stenosis (Goldblatt one-kidney, one-clip model). Animals were treated for 4 weeks, after renal artery clipping, either with an angiotensin converting enzyme inhibitor (perindopril [PER], 0.76 mg/kg/day), or with an indol derivative diuretic with specific vascular properties (indapamide [IDP], 0.24 mg/kg/day) or with the combination of both drugs at the same doses as during monotherapy. Coronary microvessel densities (arterioles and capillaries) were evaluated by double immunolabeling in nonserial cryostat sections of the left ventricular inner myocardium. After 4 weeks of hypertension (mean arterial pressure, 174+/-11 v 124+/-5 mm Hg in normotensive (NT) controls, P < .01), cardiac hypertrophy (+59%, P < .001) was associated with a significant increase in myocardial arteriolar density (+27%, P < .01), and a decrease in capillary density (-12%, P < .05). Treatment with PER prevented the increase in arterial pressure, heart weight, and arteriolar density, but did not significantly affect the low coronary capillary density in comparison with that measured in untreated hypertensive (HT) rats. Treatment with IDP preserved normal capillary myocardial density but did not significantly lower the blood pressure (BP) (169+/-9 mm Hg) and only slightly reduced the cardiac ventricular hypertrophy: - 14% v untreated HT (P < .05) and +37% v NT (P < .01). In the same way, IDP normalized the left ventricular capillary density in spontaneously HT rats (+18% v untreated rats, P < .01). The combination of both drugs, PER and IDP, at the same low doses as during monotherapy, resulted in normal levels of arterial pressure and complete normalization of cardiac hypertrophy and arteriolar and capillary myocardial densities. In conclusion, the results observed after PER suggest that blockade of the renin-angiotensin system could inhibit large coronary vessel growth but minimally affects the capillary density despite complete normalization of BP. Indapamide could have beneficial effect on myocardial capillary density. The combination of IDP and PER has additional effects and prevents the increase in BP and cardiac weight, and reverses microvascular rarefaction, specifically arteriolar and capillary densities.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/therapeutic use , Coronary Circulation/drug effects , Diuretics/therapeutic use , Hypertension, Renovascular/drug therapy , Hypertension, Renovascular/physiopathology , Indapamide/therapeutic use , Perindopril/therapeutic use , Animals , Blood Pressure/drug effects , Cardiomegaly/drug therapy , Cardiomegaly/pathology , Coronary Vessels/pathology , Drug Therapy, Combination , Male , Microcirculation/drug effects , Rats , Rats, Wistar
9.
Circulation ; 103(6): 864-70, 2001 Feb 13.
Article in English | MEDLINE | ID: mdl-11171796

ABSTRACT

BACKGROUND: Dystrophin has a key role in striated muscle mechanotransduction of physical forces. Although cytoskeletal elements play a major role in the mechanotransduction of pressure and flow in vascular cells, the role of dystrophin in vascular function has not yet been investigated. Thus, we studied endothelial and muscular responses of arteries isolated from mice lacking dystrophin (mdx mice). METHODS AND RESULTS: Carotid and mesenteric resistance arteries 120 micrometer in diameter were isolated and mounted in vitro in an arteriograph to control intraluminal pressure and flow. Blood pressure was not affected by the absence of dystrophin. Pressure-induced (myogenic), phenylephrine-induced, and KCl-induced forms of tone were unchanged. Flow (shear stress)-induced dilation in arteries isolated from mdx mice was decreased by 50% to 60%, whereas dilation to acetylcholine or sodium nitroprusside was unaffected. NG-nitro-L-arginine methyl ester-sensitive flow dilation was also decreased in arteries from mdx mice. Thus, the absence of dystrophin was associated with a defect in signal transduction of shear stress. Dystrophin was present in vascular endothelial and smooth muscle cells, as shown by immunolocalization, and localized at the level of the plasma membrane, as seen by confocal microscopy of perfused isolated arteries. CONCLUSIONS: -This is the first functional study of arteries lacking the gene for dystrophin. Vascular reactivity was normal, with the exception of flow-induced dilation. Thus, dystrophin could play a specific role in shear-stress mechanotransduction in arterial endothelial cells. Organ damage in such diseases as Duchenne dystrophy might be aggravated by such a defective arterial response to flow.


Subject(s)
Dystrophin/deficiency , Endothelium, Vascular/physiology , Muscle, Skeletal/physiology , Vasodilation , Acetylcholine/pharmacology , Analysis of Variance , Animals , Blood Flow Velocity , Blood Pressure , Calcium/pharmacology , Carotid Arteries/drug effects , Carotid Arteries/metabolism , Dystrophin/analysis , Dystrophin/genetics , Endothelium, Vascular/drug effects , Humans , In Vitro Techniques , Mesenteric Arteries/drug effects , Mesenteric Arteries/metabolism , Mice , Mice, Inbred mdx , Microscopy, Confocal , Muscle, Skeletal/blood supply , Muscle, Skeletal/drug effects , Nitroprusside/pharmacology , Phenylephrine/pharmacology , Potassium Chloride/pharmacology , Signal Transduction , Vasodilation/drug effects
10.
J Gene Med ; 2(5): 326-33, 2000.
Article in English | MEDLINE | ID: mdl-11045426

ABSTRACT

BACKGROUND: Apoptosis has been shown to contribute to myocardial reperfusion injury. It has been suggested that, in reducing the apoptotic component within the ischemic area at risk, Bcl-2 overexpression could lead to a ventricular function improvement. METHODS: Transgenic mice overexpressing the anti-apoptotic human Bcl-2 cDNA in heart were subjected to a 1-h left coronary artery occlusion followed by a 24-h reperfusion. At the end of the experiment, left ventricular function was assessed by two-dimensional echocardiography. After sacrifice, the area at risk (AR) and the infarct area (IA) were determined by Evans blue and triphenyltetrazolium chloride staining, respectively. The extent of apoptosis was assessed by the TUNEL method. Non-transgenic littermates served as controls. RESULTS: Baseline AR was not different between Bcl-2 transgenic mice and their wild-type littermates. In contrast, left ventricular ejection fraction was significantly improved in the transgenic mice line (61.25 +/- 4.0%) compared to non-transgenic littermates (43.2 +/- 5.0%, p < 0.01). This functional amelioration was correlated with a significant reduction of infarct size in transgenic animals (IA/AR 18.51 +/- 3.4% vs 50.83 +/- 8.4% in non-transgenic littermates). Finally, apoptotic nuclei were less numerous in transgenic mice than in controls as quantified by TUNEL analysis (8.1 +/- 2.2% vs 20.6 +/- 4.4%). CONCLUSIONS: Bcl-2 overexpression is effective in reducing myocardial reperfusion injury and improving heart function. This benefit correlates with a reduction of cardiomyocyte apoptosis. The apoptotic component of ischemia/reperfusion injury could therefore constitute a new therapeutic target in the acute phase of myocardial infarction.


Subject(s)
Genes, bcl-2 , Genetic Therapy/methods , Myocardial Reperfusion Injury/prevention & control , Animals , Apoptosis/genetics , Disease Models, Animal , Echocardiography , Gene Expression , Humans , Mice , Mice, Transgenic , Myocardial Infarction/therapy , Myocardial Reperfusion Injury/pathology , Myocardial Reperfusion Injury/physiopathology , Myocardium/pathology , Ventricular Function, Left
11.
Circ Res ; 87(6): 448-52, 2000 Sep 15.
Article in English | MEDLINE | ID: mdl-10988235

ABSTRACT

Ischemia induces both hypoxia and inflammation that trigger angiogenesis. The inflammatory reaction is modulated by production of anti-inflammatory cytokines. This study examined the potential role of a major anti-inflammatory cytokine, interleukin (IL)-10, on angiogenesis in a model of surgically induced hindlimb ischemia. Ischemia was produced by artery femoral occlusion in both C57BL/6J IL-10(+/+) and IL-10(-/-) mice. After 28 days, angiogenesis was quantified by microangiography, capillary, and arteriole density measurement and laser Doppler perfusion imaging. The protein levels of IL-10 and vascular endothelial growth factor (VEGF) were determined by Western blot analysis in hindlimbs. IL-10 was markedly expressed in the ischemic hindlimb of IL-10(+/+) mice. Angiogenesis in the ischemic hindlimb was significantly increased in IL-10(-/-) compared with IL-10(+/+) mice. Indeed, angiographic data showed that vessel density in the ischemic leg was 10.2+/-0.1% and 5.7+/-0.4% in IL-10(-/-) and IL-10(+/+) mice, respectively (P:<0.01). This corresponded to improved ischemic/nonischemic leg perfusion ratio by 1.4-fold in IL-10(-/-) mice compared with IL-10(+/+) mice (0.87+/-0. 05 versus 0.63+/-0.01, respectively; P:<0.01). Revascularization was associated with a 1.8-fold increase in tissue VEGF protein level in IL-10(-/-) mice compared with IL-10(+/+) mice (P:<0.01). In vivo electrotransfer of murine IL-10 cDNA in IL-10(-/-) mice significantly inhibited both the angiogenic process and the rise in VEGF protein level observed in IL-10(-/-) mice. No changes in vessel density or VEGF content were observed in the nonischemic hindlimb. These findings underscore the antiangiogenic effect of IL-10 associated with the downregulation of VEGF expression and suggest a role for the inflammatory balance in the modulation of ischemia-induced angiogenesis.


Subject(s)
Hindlimb/blood supply , Interleukin-10/metabolism , Ischemia/physiopathology , Neovascularization, Physiologic , Animals , Arterioles/physiology , Capillaries/physiology , DNA, Complementary/genetics , Endothelial Growth Factors/metabolism , Gene Transfer Techniques , Interleukin-10/genetics , Ischemia/genetics , Laser-Doppler Flowmetry , Lymphokines/metabolism , Male , Mice , Mice, Inbred C57BL , Muscle, Skeletal/metabolism , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
12.
Circulation ; 101(23): 2764-70, 2000 Jun 13.
Article in English | MEDLINE | ID: mdl-10851216

ABSTRACT

BACKGROUND: We hypothesized that, in compensated heart failure (HF), hemodynamic perturbations and their consequences exist in pulmonary artery (PA) despite the absence of any perturbation in thoracic aorta (TA). METHODS AND RESULTS: The left coronary artery was ligated in 20 male Wistar rats with compensated HF. Four months after ligation, these rats were compared with 20 sham-operated control rats. Blood pressure, velocity, viscosity, luminal diameter, and wall tensile and shear stresses were determined in PA and TA. Arterial rings were mounted in a myograph for ex vivo study. Endothelial nitric oxide synthase (eNOS) mRNA expression was determined in lung and aorta. Sections of PA and TA were used for histomorphometric study. In PA from rats with compensated HF, (1) blood pressure and wall tensile stress increased, whereas blood velocity and wall shear stress decreased; (2) contractions to KCl were not altered, but maximal contraction to phenylephrine and EC(50) decreased; (3) endothelium-dependent relaxation to acetylcholine and basal NO activity were blunted, whereas endothelium-independent relaxation was preserved; (4) eNOS mRNA levels and eNOS transcription in lung nuclei decreased; and (5) medial cross-sectional area, thickness, smooth muscle cell number, elastin, and collagen contents increased. Conversely, no such changes were found in TA from rats with compensated HF. CONCLUSIONS: In compensated HF induced by small myocardial infarction, hemodynamics, vascular wall function, and structure are altered in PA but preserved in TA. These results indicate that the pulmonary vascular bed is an early target of regional circulatory alterations in HF.


Subject(s)
Blood Pressure/physiology , Endothelium, Vascular/pathology , Heart Failure/pathology , Hypertension, Pulmonary/pathology , Pulmonary Artery/pathology , Animals , Aorta, Thoracic/pathology , Aorta, Thoracic/physiopathology , Cardiac Output , Disease Models, Animal , Endothelium, Vascular/enzymology , Endothelium, Vascular/physiopathology , Gene Expression Regulation, Enzymologic , Heart Failure/physiopathology , Hypertension, Pulmonary/physiopathology , In Vitro Techniques , Male , Myocardial Infarction/pathology , Myocardial Infarction/physiopathology , Nitric Oxide Synthase/genetics , Nitric Oxide Synthase Type III , Pulmonary Artery/physiopathology , RNA, Messenger/analysis , Rats , Rats, Wistar , Ventricular Function, Left
13.
Sante Publique ; 12(4): 485-96, 2000 Dec.
Article in French | MEDLINE | ID: mdl-11349333

ABSTRACT

Inspired by the WHO document, "Health for All in the Year 2000", and by the WHO European office's definition of 38 health objectives, several European countries have begun defining their health priorities. An analysis of the definition of these priorities shows that there exist common points among them. However, each country constructed its own process for decision making that was unique to its situation and implemented different strategies for engaging its citizens in their processes.


Subject(s)
Health Priorities/organization & administration , International Cooperation , Needs Assessment/organization & administration , Public Health , Decision Making, Organizational , Europe , Health Policy , Humans , World Health Organization
14.
Circ Res ; 85(8): e17-24, 1999 Oct 15.
Article in English | MEDLINE | ID: mdl-10521249

ABSTRACT

The potential role of anti-inflammatory cytokines in the modulation of the atherosclerotic process remains unknown. Interleukin (IL)-10 has potent deactivating properties in macrophages and T cells and modulates many cellular processes that may interfere with the development and stability of the atherosclerotic plaque. IL-10 is expressed in human atherosclerosis and is associated with decreased signs of inflammation. In the present study, we show that IL-10-deficient C57BL/6J mice fed an atherogenic diet and raised under specific pathogen-free conditions exhibit a significant 3-fold increase in lipid accumulation compared with wild-type mice. Interestingly, the susceptibility of IL-10-deficient mice to atherosclerosis was exceedingly high (30-fold increase) when the mice were housed under conventional conditions. Atherosclerotic lesions of IL-10-deficient mice showed increased T-cell infiltration, abundant interferon-gamma expression, and decreased collagen content. In vivo, transfer of murine IL-10 achieved 60% reduction in lesion size. These results underscore the critical roles of IL-10 in both atherosclerotic lesion formation and stability. Moreover, IL-10 appears to be crucial as a protective factor against the effect of environmental pathogens on atherosclerosis.


Subject(s)
Arteriosclerosis/immunology , Interleukin-10/deficiency , Animals , Arteriosclerosis/pathology , Arteriosclerosis/therapy , Cholesterol/blood , Diet, Atherogenic , Female , Interleukin-10/therapeutic use , Mice , Mice, Inbred C57BL
15.
J Cardiovasc Pharmacol ; 34(1): 102-7, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10413075

ABSTRACT

Systemic arterial elastic properties, important determinants of left ventricular function and coronary blood flow, are compromised in myocardial infarction (MI). The cardiac effect of angiotensin-converting enzyme inhibitors (ACEIs) has been extensively studied, whereas their arterial effect has been poorly reported in MI. The aim of this work was to study the effect of prolonged ACEI enalapril treatment on systemic arterial structure and elastic properties in rats with MI. One week after the induction of an MI, 40 male Wistar rats received either no treatment (n = 20) or ACEI enalapril (2 mg/kg; n = 20) for 17 weeks. At the end of the treatment period, blood pressure, cardiac output, total peripheral resistance, systemic arterial compliance, characteristic impedance, and left ventricular power were measured in anesthetized rats. Then the rats were killed for infarct-size determination and aortic histomorphometric study. Infarct size, heart, and left and right ventricular weights were similar in the ACEI-treated and untreated infarcted rats. Prolonged ACEI enalapril treatment reduced blood pressure by 17% (p < 0.001), total peripheral resistance by 22% (p < 0.01), and characteristic impedance by 26% (p < 0.03), and increased systemic arterial compliance by 35% (p < 0.01), in comparison with untreated infarcted rats. Enalapril reduced aortic media wall thickness by 9% (p < 0.02) and increased elastin content by 22% (p < 0.03) and elastin-to-collagen content ratio by 42% (p < 0.01). Enalapril did not affect cardiac output and left ventricular power. Smooth muscle cell nuclei number and size and collagen content of aortic wall were similar in the ACEI-treated and untreated infarcted rats. These results indicate that long-term treatment with ACEI enalapril improves arterial elastic properties through structural modifications of arterial wall in rats with MI. This vascular effect may contribute to improve the left ventricular function and the coronary perfusion of infarcted myocardium, and added to the cardiac effect, may explain the prevention of left ventricular remodeling observed with ACEI in this model.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/therapeutic use , Arteries/drug effects , Enalapril/therapeutic use , Hemodynamics/drug effects , Myocardial Infarction/drug therapy , Anesthesia , Animals , Body Weight/drug effects , Enalapril/pharmacology , Male , Organ Size/drug effects , Rats , Rats, Wistar , Time Factors
16.
Proc Natl Acad Sci U S A ; 95(3): 1301-6, 1998 Feb 03.
Article in English | MEDLINE | ID: mdl-9448326

ABSTRACT

Aging is associated with cardiac hypertrophy and arterial stiffening possibly associated with accumulation of advanced glycation end products (AGEs). We evaluated the effect of aminoguanidine, an inhibitor of AGE production, on end-stage alterations of renal and cardiovascular systems. Normotensive WAG/Rij rats were treated from 24 to 30 mo with aminoguanidine and compared with a control group. Aminoguanidine did not modify body and kidney weights but prevented the age-related cardiac hypertrophy (heart weight: 1276 +/- 28 mg and 1896 +/- 87 mg in 24- and 30-mo-old control animals and 1267 +/- 60 mg in 30-mo-old treated rats, P < 0.01). The increase in mesangial surface in aging rats was reduced by 30% by aminoguanidine. Collagen content of the arterial wall increased between 24 and 30 mo whereas elastin content, media thickness, and smooth muscle cell number remained unchanged. Aminoguanidine did not affect these parameters; however, the age-related increase in aortic impedance (12.4 +/- 1.4 and 18.2 +/- 1.9 10(3).dyne.sec.cm-5 in control 24- and 30-mo-old rats, P < 0.01) and the decrease in carotid distensibility (0.79 +/- 0.11 and 0.34 +/- 0. 07 mm Hg-1 in control 24- and 30-mo-old rats, P < 0.01) were prevented by aminoguanidine. The prevention of arterial stiffening and cardiac hypertrophy in the absence of changes in collagen and elastin content suggests that the effect of aminoguanidine is related to a decrease in the AGE-induced cross-linking of the extracellular matrix.


Subject(s)
Aging/drug effects , Aorta/drug effects , Cardiomegaly/metabolism , Enzyme Inhibitors/pharmacology , Guanidines/pharmacology , Aging/pathology , Animals , Aorta/metabolism , Aorta/pathology , Blood Pressure/drug effects , Cardiac Output/drug effects , Cardiomegaly/pathology , Carotid Arteries/drug effects , Carotid Arteries/metabolism , Carotid Arteries/pathology , Elasticity/drug effects , Extracellular Matrix Proteins/metabolism , Glycation End Products, Advanced/metabolism , Kidney/drug effects , Kidney/metabolism , Kidney/pathology , Longevity/drug effects , Male , Rats
17.
Arterioscler Thromb Vasc Biol ; 17(11): 3196-201, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9409311

ABSTRACT

We previously showed that chronic angiotensin-converting enzyme (ACE) inhibition prevented the increase in aortic collagen in spontaneously hypertensive rats (SHRs) independently of blood pressure reduction. The aim of the present study was to determine whether the effects of ACE inhibition on aortic fibrosis were due to inhibition of angiotensin II formation, preservation of bradykinin, or a combination of both. Four week-old SHRs were treated for 4 months with the ACE inhibitor quinapril, quinapril with the bradykinin B2 receptor antagonist Hoe 140, or the angiotensin II AT1 receptor antagonist CI996. Control SHR and Wistar-Kyoto (WKY) rats received a placebo for the same period of time. At the end of the treatment, as compared to conscious SHR and WKY controls, quinapril completely prevented the development of hypertension, whereas quinapril-Hoe 140 and the AT1 receptor antagonist produced only a partial reduction of blood pressure. In relation with blood pressure changes, aortic hypertrophy was significantly prevented by quinapril but not by quinapril-Hoe 140 or CI996. In contrast, aortic collagen accumulation was completely prevented by all three treatments. The study provides evidence that in young live SHRs, the prevention of aortic collagen accumulation is independent of blood pressure changes and bradykinin preservation and involves exclusively angiotensin II inhibition through AT1 receptors.


Subject(s)
Angiotensin II/physiology , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Aorta, Thoracic/drug effects , Bradykinin/analogs & derivatives , Bradykinin/physiology , Collagen/metabolism , Hypertension/metabolism , Imidazoles/pharmacology , Isoquinolines/pharmacology , Tetrahydroisoquinolines , Tetrazoles/pharmacology , Angiotensin II/antagonists & inhibitors , Angiotensin Receptor Antagonists , Angiotensin-Converting Enzyme Inhibitors/administration & dosage , Angiotensin-Converting Enzyme Inhibitors/therapeutic use , Animals , Aorta, Thoracic/metabolism , Aorta, Thoracic/pathology , Bradykinin/administration & dosage , Bradykinin/antagonists & inhibitors , Bradykinin/pharmacology , Bradykinin/therapeutic use , Bradykinin Receptor Antagonists , Drug Therapy, Combination , Hemodynamics/drug effects , Hypertension/drug therapy , Hypertension/genetics , Hypertension/prevention & control , Hypertrophy , Imidazoles/administration & dosage , Imidazoles/therapeutic use , Isoquinolines/administration & dosage , Isoquinolines/therapeutic use , Male , Quinapril , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Receptor, Angiotensin, Type 1 , Receptor, Angiotensin, Type 2 , Receptor, Bradykinin B2 , Tetrazoles/administration & dosage , Tetrazoles/therapeutic use
18.
Respir Med ; 91(8): 496-8, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9338054

ABSTRACT

Tracheobronchopathia osteochondroplastica (TO) is a rare and usually benign disorder affecting the trachea and occasionally the bronchi. We describe the case of a 46-year-old woman who was discovered to have TO at the time of a difficult intubation. This case was also unusual since the patient had presented no previous symptoms despite the presence of extensive endotracheal and bronchial lesions. The incidence of TO appears to be underestimated in the literature in view of the fact that it is usually benign. However, a more accurate estimate of its true prevalence may become available through the use of bronchoscopy and computerized tomographic scanning.


Subject(s)
Bone and Bones , Bronchial Diseases/pathology , Cartilage , Choristoma/pathology , Tracheal Diseases/pathology , Female , Humans , Intubation, Intratracheal , Middle Aged
19.
J Hypertens ; 15(3): 251-8, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9468452

ABSTRACT

BACKGROUND: Reduction in sodium intake improves the survival of stroke-prone spontaneously hypertensive rats (SHR-SP) without causing any change in their blood pressure. OBJECTIVE: To investigate whether the diuretic indapamide improves survival of SHR-SP and whether changes in the structure and the function of large arteries are associated with survival. EXPERIMENTAL DESIGN: Forty-eight hypertensive rats aged 6 weeks were divided into three groups: a control SHR-SP group (n = 24) and a control spontaneously hypertensive rat (SHR) group (n = 12), with 1% saline drinking water; and an indapamide-treated SHR-SP group (n = 12) with 1% saline drinking water administered 1 mg/kg per day indapamide via their food. At the end of a 12-week follow-up period, pulsatile changes in blood pressure and common carotid artery diameter (measured by high-resolution echo-tracking techniques) were determined and aortic histomorphometry was performed. RESULTS: By the end of the study 58% of the SHR-SP control group rats had died. There were no deaths in the other two groups. In these two groups the mean blood pressure (217+/-10 and 212+/-7 mmHg), carotid diameter and distensibility (0.48+/-0.09 and 0.61+/-0.22 mmHg[-1]), arterial thickness (116+/-4 and 116+/-3 microm), and collagen content of the arterial wall were identical. In the SHR-SP control group the mean blood pressure was significantly lower (168+/-9 mmHg), the carotid distensibility was higher (1.47+/-0.35 mmHg[-1]), and the arterial thickness (138+/-5 microm) and collagen content were substantially higher than those in the other two groups. In the study population as a whole, for a given mean arterial pressure the carotid distensibility was identical in the three groups, although the arterial thickness was substantially greater in the SHR-SP control group rats. CONCLUSIONS: The study provides evidence that the diuretic compound indapamide improved the survival of SRH-SP even though their blood pressure was higher than that of untreated animals, and that genetic sensitivity to sodium, rather than blood pressure, influences the changes in arterial structure.


Subject(s)
Carotid Artery, Common/drug effects , Carotid Artery, Common/physiopathology , Cerebrovascular Disorders/genetics , Hypertension/genetics , Hypertension/physiopathology , Sodium/pharmacology , Animals , Blood Pressure/drug effects , Body Weight/drug effects , Body Weight/physiology , Collagen/metabolism , Diuretics/therapeutic use , Genetic Predisposition to Disease , Hypertension/drug therapy , Indapamide/therapeutic use , Male , Rats , Rats, Inbred SHR/genetics , Survival Analysis , Vasomotor System/physiopathology
20.
J Clin Invest ; 100(11): 2909-14, 1997 Dec 01.
Article in English | MEDLINE | ID: mdl-9389758

ABSTRACT

The intermediate filament vimentin might play a key role in vascular resistance to mechanical stress. We investigated the responses to pressure (tensile stress) and flow (shear stress) of mesenteric resistance arteries perfused in vitro from vimentin knockout mice. Arteries were isolated from homozygous (Vim-/-, n = 14) or heterozygous vimentin-null mice (Vim+/-, n = 5) and from wild-type littermates (Vim+/+, n = 9). Passive arterial diameter (175+/-15 micron in Vim+/+ at 100 mmHg) and myogenic tone were not affected by the absence of vimentin. Flow-induced (0-150 microl/min) dilation (e. g., 19+/-3 micron dilation at 150 mmHg in Vim+/+) was significantly attenuated in Vim-/- mice (13+/-2 micron dilation, P < 0.01). Acute blockade of nitric oxide synthesis (NG-nitro- L-arginine, 10 microM) significantly decreased flow-induced dilation in both groups, whereas acute blockade of prostaglandin synthesis (indomethacin, 10 microM) had no significant effect. Mean blood pressure, in vivo mesenteric blood flow and diameter, and mesenteric artery media thickness or media to lumen ratio were not affected by the absence of vimentin. Thus, the absence of vimentin decreased selectively the response of resistance arteries to flow, suggesting a role for vimentin in the mechanotransduction of shear stress.


Subject(s)
Mesenteric Arteries/physiology , Vimentin/physiology , Animals , Female , In Vitro Techniques , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Knockout , Stress, Mechanical , Tensile Strength , Vascular Resistance , Vasodilation , Vimentin/deficiency , Vimentin/genetics
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