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1.
J Cancer ; 8(15): 3078-3085, 2017.
Article in English | MEDLINE | ID: mdl-28928899

ABSTRACT

Background: Non-invasive tumor characterization and monitoring are among the key goals of medical imaging. Using hyperpolarized 13C-labelled metabolic probes fast metabolic pathways can be probed in real-time, providing new opportunities for tumor characterization. In this in vitro study, we investigated whether measurement of apparent diffusion coefficient (ADC) measurements and magnetic resonance spectroscopy (MRS) of co-polarized 13C-labeled pyruvic acid and fumaric acid can non-invasively detect both necrosis and changes in lactate export, which are parameters indicative of tumor aggressiveness. Methods:13C-labeled pyruvic acid and fumaric acid were co-polarized in a preclinical hyperpolarizer and the dissolved compounds were added to prepared samples of 8932 pancreatic cancer and MCF-7 breast carcinoma cells. Extracellular lactate concentrations and cell viability were measured in separate assays. Results: The mean ratios of the ADC values of lactate and pyruvate (ADClac/ADCpyr) between MCF-7 (0.533 ± 0.015, n = 3) and 8932 pancreatic cancer cells (0.744 ± 0.064, n = 3) showed a statistically significant difference (p = 0.048). 8932 cells had higher extracellular lactate concentrations in the extracellular medium (22.97 ± 2.53 ng/µl) compared with MCF-7 cells (7.52 ± 0.59 ng/µl; p < 0.001). Fumarate-to-malate conversion was only detectable in necrotic cells, thereby allowing clear differentiation between necrotic and viable cells. Conclusion: We provide evidence that MRS of hyperpolarized 13C-labelled pyruvic acid and fumaric acid, with their respective conversions to lactate and malate, are useful for characterization of necrosis and lactate efflux in tumor cells.

2.
NMR Biomed ; 29(8): 1079-87, 2016 08.
Article in English | MEDLINE | ID: mdl-27348729

ABSTRACT

Most tumours exhibit a high rate of glycolysis and predominantly produce energy by lactic acid fermentation. To maintain energy production and prevent toxicity, the lactate generated needs to be rapidly transported out of the cell. This is achieved by monocarboxylate transporters (MCTs), which therefore play an essential role in cancer metabolism and development. In vivo experiments were performed on eight male Fisher F344 rats bearing a subcutaneous mammary carcinoma after injection of hyperpolarised [1-(13) C]pyruvate. A Gd(III)DO3A complex that binds to pyruvate and its metabolites was used to efficiently destroy the extracellular magnetisation after hyperpolarised lactate had been formed. Moreover, a pulse sequence including a frequency-selective saturation pulse was designed so that the pyruvate magnetisation could be destroyed to exclude effects arising from further conversion. Given this preparation, metabolite transport out of the cell manifested as additional decay and apparent cell membrane transporter rates could thus be obtained using a reference measurement without a relaxation agent. In addition to slice-selective spectra, spatially resolved maps of apparent membrane transporter activity were acquired using a single-shot spiral gradient readout. A considerable increase in decay rate was detected for lactate, indicating rapid transport out of the cell. The alanine signal was unaltered, which corresponds to a slower efflux rate. This technique could allow for better understanding of tumour metabolism and progression, and enable treatment response measurements for MCT-targeted cancer therapies. Moreover, it provides vital insights into the signal kinetics of hyperpolarised [1-(13) C]pyruvate examinations. Copyright © 2016 John Wiley & Sons, Ltd.


Subject(s)
Lactic Acid/metabolism , Molecular Imaging/methods , Neoplasms, Experimental/metabolism , Neoplasms, Experimental/pathology , Pyruvic Acid/metabolism , Animals , Biomarkers, Tumor/metabolism , Cell Line, Tumor , Magnetic Resonance Imaging/methods , Male , Molecular Probe Techniques , Neoplasms, Experimental/diagnostic imaging , Rats , Rats, Inbred F344 , Reproducibility of Results , Sensitivity and Specificity
3.
NMR Biomed ; 29(7): 952-60, 2016 07.
Article in English | MEDLINE | ID: mdl-27195474

ABSTRACT

Individual tumor characterization and treatment response monitoring based on current medical imaging methods remain challenging. This work investigates hyperpolarized (13) C compounds in an orthotopic rat hepatocellular carcinoma (HCC) model system before and after transcatheter arterial embolization (TAE). HCC ranks amongst the top six most common cancer types in humans and accounts for one-third of cancer-related deaths worldwide. Early therapy response monitoring could aid in the development of personalized therapy approaches and novel therapeutic concepts. Measurements with selectively (13) C-labeled and hyperpolarized urea, pyruvate and fumarate were performed in tumor-bearing rats before and after TAE. Two-dimensional, slice-selective MRSI was used to obtain spatially resolved maps of tumor perfusion, cell energy metabolic conversion rates and necrosis, which were additionally correlated with immunohistochemistry. All three injected compounds, taken together with their respective metabolites, exhibited similar signal distributions. TAE induced a decrease in blood flow into the tumor and thus a decrease in tumor to muscle and tumor to liver ratios of urea, pyruvate and its metabolites, alanine and lactate, whereas conversion rates remained stable or increased on TAE in tumor, muscle and liver tissue. Conversion from fumarate to malate successfully indicated individual levels of necrosis, and global malate signals after TAE suggested the washout of fumarase or malate itself on necrosis. This study presents a combination of three (13) C compounds as novel candidate biomarkers for a comprehensive characterization of genetically and molecularly diverse HCC using hyperpolarized MRSI, enabling the simultaneous detection of differences in tumor perfusion, metabolism and necrosis. If, as in this study, bolus dynamics are not required and qualitative perfusion information is sufficient, the desired information could be extracted from hyperpolarized fumarate and pyruvate alone, acquired at higher fields with better spectral separation. Copyright © 2016 John Wiley & Sons, Ltd.


Subject(s)
Carbon-13 Magnetic Resonance Spectroscopy/methods , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/therapy , Embolization, Therapeutic/methods , Molecular Imaging/methods , Organic Chemicals/metabolism , Animals , Carcinoma, Hepatocellular/diagnosis , Cell Line, Tumor , Female , Magnetic Resonance Imaging/methods , Rats , Reproducibility of Results , Sensitivity and Specificity , Treatment Outcome
4.
Magn Reson Med ; 76(6): 1900-1904, 2016 12.
Article in English | MEDLINE | ID: mdl-26822562

ABSTRACT

PURPOSE: We characterized the performance of a novel hyperpolarized perfusion marker, α-trideuteromethyl[15N]glutamine, for direct comparison with a 13C-based hyperpolarized perfusion marker, [13C, 15N2]urea. METHODS: A hardware platform and pulse sequence for in vivo 15N experiments were established. Hyperpolarized solutions of α-trideuteromethyl[15N]glutamine and [13C, 15N2]urea were injected into healthy male Lewis rats. Kidney slice images were acquired using a single-shot spiral readout. Both compounds were compared to determine in vivo signal lifetime and tracer distribution. Mass spectrometry was performed to evaluate excretion of the compound. RESULTS: Compared with 13C-labeled urea, a significantly increased signal lifetime was observed. While the urea signal was gone after 90 s, decay of the glutamine compound was sufficiently slow to obtain a quantifiable signal, even after 5 min. The glutamine derivative showed strong localization in the kidneys with little background signal. Effective T1 of α-trideuteromethyl[15N]glutamine was approximately eight-fold higher than that of urea. Mass spectrometry results confirmed rapid excretion within the time scale of the measurement. CONCLUSION: Hyperpolarized α-trideuteromethyl[15N]glutamine is a highly promising candidate for renal studies because of its long signal lifetime, strong localization and rapid excretion. Magn Reson Med 76:1900-1904, 2016. © 2016 International Society for Magnetic Resonance in Medicine.


Subject(s)
Glutamine/pharmacokinetics , Kidney Function Tests/methods , Kidney/metabolism , Magnetic Resonance Spectroscopy/methods , Molecular Imaging/methods , Urea/pharmacokinetics , Animals , Biomarkers/metabolism , Carbon Isotopes/pharmacokinetics , Kinetics , Male , Metabolic Clearance Rate , Radiopharmaceuticals/pharmacokinetics , Rats , Rats, Inbred Lew , Reproducibility of Results , Sensitivity and Specificity
5.
NMR Biomed ; 28(6): 715-25, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25908233

ABSTRACT

The aim of this study was to characterise and compare widely used acquisition strategies for hyperpolarised (13)C imaging. Free induction decay chemical shift imaging (FIDCSI), echo-planar spectroscopic imaging (EPSI), IDEAL spiral chemical shift imaging (ISPCSI) and spiral chemical shift imaging (SPCSI) sequences were designed for two different regimes of spatial resolution. Their characteristics were studied in simulations and in tumour-bearing rats after injection of hyperpolarised [1-(13)C]pyruvate on a clinical 3-T scanner. Two or three different sequences were used on the same rat in random order for direct comparison. The experimentally obtained lactate signal-to-noise ratio (SNR) in the tumour matched the simulations. Differences between the sequences were mainly found in the encoding efficiency, gradient demand and artefact behaviour. Although ISPCSI and SPCSI offer high encoding efficiencies, these non-Cartesian trajectories are more prone than EPSI and FIDCSI to artefacts from various sources. If the encoding efficiency is sufficient for the desired application, EPSI has been proven to be a robust choice. Otherwise, faster spiral acquisition schemes are recommended. The conclusions found in this work can be applied directly to clinical applications.


Subject(s)
Algorithms , Carbon-13 Magnetic Resonance Spectroscopy/methods , Molecular Imaging/methods , Neoplasms, Experimental/metabolism , Pyruvic Acid/pharmacokinetics , Signal Processing, Computer-Assisted , Animals , Cell Line, Tumor , Humans , Information Storage and Retrieval/methods , Neoplasms, Experimental/pathology , Rats , Rats, Inbred F344 , Reproducibility of Results , Sensitivity and Specificity
6.
J Magn Reson ; 243: 40-6, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24717443

ABSTRACT

Dynamic nuclear polarisation has enabled real-time metabolic imaging of pyruvate and its metabolites. Conventional imaging sequences rely on predefined settings and do not account for intersubject variations in biological parameters such as perfusion. We present a fully automatic real-time bolus tracking sequence for hyperpolarised substrates which starts the imaging acquisition at a defined point on the bolus curve. This reduces artefacts due to signal change and allows for a more efficient use of hyperpolarised magnetisation. For single time point imaging methods, bolus tracking enables a more reliable and consistent quantification of metabolic activity. An RF excitation with a small flip angle is used to obtain slice-selective pyruvate tracking information in rats. Moreover, in combination with a copolarised urea and pyruvate injection, spectrally selective tracking on urea allows obtaining localised bolus tracking information without depleting the pyruvate signal. Particularly with regard to clinical application, the bolus tracking technique could provide an important step towards a routine assessment protocol which removes operator dependencies and ensures comparable results.


Subject(s)
Kidney/metabolism , Magnetic Resonance Spectroscopy/methods , Models, Biological , Pyruvic Acid/pharmacokinetics , Animals , Carbon Isotopes/chemistry , Carbon Isotopes/pharmacokinetics , Computer Simulation , Isotope Labeling , Metabolic Clearance Rate , Pyruvic Acid/chemistry , Rats , Reproducibility of Results , Sensitivity and Specificity
7.
J Magn Reson ; 227: 35-8, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23262330

ABSTRACT

Scalar coupling relaxation, which is usually only associated with closely resonant nuclei (e.g., (79)Br-(13)C), can be a very effective relaxation mechanism. While working on hyperpolarized [5-(13)C]glutamine, fast liquid-state polarization decay during transfer to the MRI scanner was observed. This behavior could hypothetically be explained by substantial T(1) shortening due to a scalar coupling contribution (type II) to the relaxation caused by the fast-relaxing quadrupolar (14)N adjacent to the (13)C nucleus in the amide group. This contribution is only effective in low magnetic fields (i.e., less than 800 µT) and prevents the use of molecules bearing the (13)C-amide group as hyperpolarized MRS/MRI probes. In the present work, this hypothesis is explored both theoretically and experimentally. The results show that high hyperpolarization levels can be retained using either a (15)N-labeled amide or by applying a magnetic field during transfer of the sample from the polarizer to the MRI scanner.


Subject(s)
Amides/chemistry , Carbon Isotopes/chemistry , Earth, Planet , Magnetic Resonance Spectroscopy/methods , Magnetometry/methods , Nitrogen/chemistry , Amides/radiation effects , Carbon Isotopes/radiation effects , Magnetic Fields , Nitrogen/radiation effects
8.
Magn Reson Med ; 69(5): 1209-16, 2013 May.
Article in English | MEDLINE | ID: mdl-22648928

ABSTRACT

Within the last decade hyperpolarized [1-13C] pyruvate chemical-shift imaging has demonstrated impressive potential for metabolic MR imaging for a wide range of applications in oncology, cardiology, and neurology. In this work, a highly efficient pulse sequence is described for time-resolved, multislice chemical shift imaging of the injected substrate and obtained downstream metabolites. Using spectral-spatial excitation in combination with single-shot spiral data acquisition, the overall encoding is evenly distributed between excitation and signal reception, allowing the encoding of one full two-dimensional metabolite image per excitation. The signal-to-noise ratio can be flexibly adjusted and optimized using lower flip angles for the pyruvate substrate and larger ones for the downstream metabolites. Selectively adjusting the excitation of the down-stream metabolites to 90° leads to a so-called "saturation-recovery" scheme with the detected signal content being determined by forward conversion of the available pyruvate. In case of repetitive excitations, the polarization is preserved using smaller flip angles for pyruvate. Metabolic exchange rates are determined spatially resolved from the metabolite images using a simplified two-site exchange model. This novel contrast is an important step toward more quantitative metabolic imaging. Goal of this work was to derive, analyze, and implement this "saturation-recovery metabolic exchange rate imaging" and demonstrate its capabilities in four rats bearing subcutaneous tumors.


Subject(s)
Alanine/metabolism , Bicarbonates/metabolism , Lactic Acid/metabolism , Magnetic Resonance Spectroscopy/methods , Neoplasms, Experimental/metabolism , Pyruvic Acid/pharmacokinetics , Animals , Carbon Isotopes/pharmacokinetics , Cell Line, Tumor , Female , Metabolic Clearance Rate , Neoplasms, Experimental/diagnosis , Protons , Radiopharmaceuticals/pharmacokinetics , Rats , Rats, Inbred F344
9.
NMR Biomed ; 26(5): 557-68, 2013 May.
Article in English | MEDLINE | ID: mdl-23233311

ABSTRACT

The detection of tumors noninvasively, the characterization of their progression by defined markers and the monitoring of response to treatment are goals of medical imaging techniques. In this article, a method which measures the apparent diffusion coefficients (ADCs) of metabolites using hyperpolarized (13) C diffusion-weighted spectroscopy is presented. A pulse sequence based on the pulsed gradient spin echo (PGSE) was developed that encodes both kinetics and diffusion information. In experiments with MCF-7 human breast cancer cells, we detected an ADC of intracellularly produced lactate of 1.06 ± 0.15 µm(2) /ms, which is about one-half of the value measured with pyruvate in extracellular culture medium. When monitoring tumor cell spheroids during progressive membrane permeabilization with Triton X-100, the ratio of lactate ADC to pyruvate ADC increases as the fraction of dead cells increases. Therefore, (13) C ADC detection can yield sensitive information on changes in membrane permeability and subsequent cell death. Our results suggest that both metabolic label exchange and (13) C ADCs can be acquired simultaneously, and may potentially serve as noninvasive biomarkers for pathological changes in tumor cells.


Subject(s)
Magnetic Resonance Spectroscopy/methods , Neoplasms/metabolism , Carbon Isotopes , Cell Line, Tumor , Cell Membrane Permeability , Diffusion , Female , Humans , Neoplasms/pathology , Pyruvic Acid/metabolism , Spheroids, Cellular
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