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1.
Chem Commun (Camb) ; 51(57): 11482-4, 2015 Jul 21.
Article in English | MEDLINE | ID: mdl-26091539

ABSTRACT

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys-Cys-X-X-Cys-Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized (129)Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the (129)Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.


Subject(s)
Cysteine/analysis , Fluoresceins/chemistry , Fluorescent Dyes/chemistry , Organometallic Compounds/chemistry , Peptides/analysis , Polycyclic Compounds/chemistry , Amino Acid Sequence , Biosensing Techniques , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Recombinant Proteins/analysis , Spectrometry, Fluorescence , Xenon Isotopes/chemistry
2.
Magn Reson Chem ; 51(1): 19-31, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23132654

ABSTRACT

Cryptophane-C is composed of two nonequivalent cyclotribenzylene caps, one of which contains methoxy group substituents on the phenyl rings. The two caps are connected by three OCH(2)CH(2)O linkers in an anti arrangement. Host-guest complexes of cryptophane-C with dichloromethane and chloroform in solution were investigated in detail by nuclear magnetic resonance techniques and density functional theory (DFT) calculations. Variable temperature proton and carbon-13 spectra show a variety of dynamic processes, such as guest exchange and host conformational transitions. The guest exchange was studied quantitatively by exchange spectroscopy measurements or by line-shape analysis. The conformational preferences of the guest-containing host were interpreted through cross-relaxation measurements, providing evidence of the gauche+2 and gauche-2 conformations of the linkers. In addition, the mobility of the chloroform guest inside the cavity was studied by carbon-13 relaxation experiments. Combining different types of evidence led to a detailed picture of molecular recognition, interpreted in terms of conformational selection.

3.
Magn Reson Chem ; 48(8): 623-9, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20589729

ABSTRACT

Guest-host complex between cryptophane C, possessing two non-equivalent caps, and chloroform is investigated by NMR spectroscopy. The kinetics of the chloroform exchange between the bound and free sites is determined by (1)H exchange spectroscopy. Moreover, the preferential orientation of chloroform molecule with respect to the cryptophane C frame is examined by the NOESY and ROESY experiments. The experimental findings are compared to the results of quantum chemical calculations.


Subject(s)
Bridged-Ring Compounds/chemistry , Chloroform/chemistry , Phenyl Ethers/chemistry , Kinetics , Magnetic Resonance Spectroscopy/standards , Models, Molecular , Molecular Structure , Quantum Theory , Reference Standards
4.
J Mass Spectrom ; 36(10): 1092-7, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11747102

ABSTRACT

The formation of cryptophane-A (C1) and the deuterated cryptophanes C2-C6 from their respective precursors P1-P6 in a mass spectrometer ion-source was evidenced by liquid secondary ion mass spectrometry (LSIMS). Mass-analyzed ion kinetic energy experiments performed on the precursor molecular ions suggested that cryptophane formation occurred mainly in the liquid-matrix before desorption rather than in the gas phase. In addition, we observed that the presence of cations, such as lithium or sodium ions, inhibited the formation of the cryptophane molecular ions. In the light of these results we used the LSIMS technique to investigate the formation of the new cryptophanes C7-C13. All the data collected support the idea that a direct comparison can be made between these experimental findings and chemistry in solution.

5.
Biochemistry ; 37(9): 3149-55, 1998 Mar 03.
Article in English | MEDLINE | ID: mdl-9485468

ABSTRACT

We have investigated in the present study the interaction between G-actin and various types of liposomes, zwitterionic, positively charged, and negatively charged. To investigate at the molecular level the conformation of actin in the presence of lipids, we have selectively attached a fluorinated probe, 3-bromo-1,1,1-trifluoropropanone, to the actin cysteine residues 10, 285, and 374 and used high-resolution 19F nuclear magnetic resonance spectroscopy to investigate the probe resonances. The results indicate a change in the mobility of the 19F labels when G-actin is in the presence of positively charged liposomes made of DMPC and stearylamine and in the presence of DMPG, a negatively charged lipid. No conformational change was observed in the actin molecule in the presence of neutral liposomes. Electron micrographs of these systems reveal the formation of paracrystalline arrays of actin filaments at the surface of the positively charged liposomes, while no evidence of actin polymerization or paracrystallization was observed in the presence of DMPG. The interaction between actin and the lipid polar headgroup has also been investigated using solid-state phosphorus and deuterium NMR. The results indicate no evidence of interaction between actin and zwitterionic liposomes but show an interaction between the positively charged liposomes and a negative charge on the actin molecules. Interestingly, the negatively charged liposomes interact with a positive charge, which is most likely associated with the three residues (His-Arg-Lys) preceding the cysteine 374 residue in the protein.


Subject(s)
Actins/metabolism , Deuterium/metabolism , Fluorine Radioisotopes/metabolism , Liposomes/metabolism , Phosphorus/metabolism , Acetone/analogs & derivatives , Acetone/metabolism , Indicators and Reagents/metabolism , Magnetic Resonance Spectroscopy , Microscopy, Electron , Protein Conformation
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