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1.
J Virol ; 75(11): 5363-9, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11333918

ABSTRACT

The fusion (F) protein of the paramxyovirus simian parainfluenza virus 5 (SV5) promotes virus-cell and cell-cell membrane fusion. Previous work had indicated that removal of the SV5 F protein cytoplasmic tail (F Tail- or FDelta19) caused a block in fusion promotion at the hemifusion stage. Further examination has shown that although the F Tail- mutant is severely debilitated in promotion of fusion as measured by using two reporter gene assays and is debilitated in the formation of syncytia relative to the wild-type F protein, the F Tail- mutant is capable of promoting the transfer of small aqueous dyes. These data indicate that F Tail- is fully capable of promoting formation of small fusion pores. However, enlargement of fusion pores is debilitated, suggesting that either the cytoplasmic tail of the F protein plays a direct role in pore expansion or that it interacts with other components which control pore growth.


Subject(s)
Membrane Fusion , Rubulavirus/genetics , Viral Fusion Proteins/genetics , Animals , Biological Transport , Cell Line , Coloring Agents , Erythrocyte Membrane/metabolism , Erythrocyte Membrane/pathology , Erythrocytes/metabolism , Giant Cells , Humans , Lipid Metabolism , Mutation , Transfection , Viral Fusion Proteins/analysis , Viral Fusion Proteins/metabolism
2.
Virology ; 281(1): 138-50, 2001 Mar 01.
Article in English | MEDLINE | ID: mdl-11222104

ABSTRACT

The fusion (F) protein of the paramyxovirus SV5 promotes both virus-cell and cell-cell fusion. Recently, the atomic structure at 1.4 A of an extremely thermostable six-helix bundle core complex consisting of two heptad repeat regions of the F protein has been described (K. A. Baker, R. E. Dutch, R. A. Lamb, and T. S Jardetsky, Mol. Cell 3, 309-319, 1999). To analyze the conformations of the F protein at various stages of the membrane fusion process and to understand further the role of formation of the six-helix bundle core complex in promotion of membrane fusion, antibodies to peptides corresponding to regions of the F protein were obtained. Major changes in F protein antibody recognition were found after cleavage of the precursor protein F(0) to the fusogenically active disulfide-linked heterodimer, F(1) + F(2), and antibodies directed against the heptad repeat regions recognized only the uncleaved form. A monoclonal antibody directed against the F protein showed increased recognition at the cell surface of the cleaved form of the F protein as compared to uncleaved F protein, again indicating changes in conformation between the uncleaved and cleaved forms of the F protein. Anti-peptide antibodies specific for the heptad repeat regions were unable to precipitate a synthetic protein that consisted of the heptad repeat regions separated only by a small spacer, suggesting that the antibodies are unable to recognize their target regions when the heptad repeats are present in the six-helix bundle core complex. Taken together, these data indicate that the six-helix bundle core complex is not present in the precursor molecule F(0) and that significant conformational changes occur subsequent to cleavage of the F protein.


Subject(s)
Respirovirus , Viral Fusion Proteins/chemistry , Viral Fusion Proteins/metabolism , Animals , Antibodies, Monoclonal/immunology , Antibodies, Viral/immunology , Antibody Specificity , Cell Line , Cross-Linking Reagents , Dimerization , Disulfides/metabolism , Flow Cytometry , HeLa Cells , Humans , Immune Sera/immunology , Membrane Fusion , Molecular Weight , Mutation , Papain/metabolism , Peptide Fragments/immunology , Precipitin Tests , Protein Conformation , Recombinant Fusion Proteins , Repetitive Sequences, Amino Acid/immunology , Succinimides , Trypsin/metabolism , Viral Fusion Proteins/genetics , Viral Fusion Proteins/immunology
3.
Biosci Rep ; 20(6): 597-612, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11426696

ABSTRACT

Fusion proteins from a group of widely disparate viruses, including the paramyxovirus F protein, the HIV and SIV gp160 proteins, the retroviral Env protein, the Ebola virus Gp, and the influenza virus haemagglutinin, share a number of common features. All contain multiple glycosylation sites, and must be trimeric and undergo proteolytic cleavage to be fusogenically active. Subsequent to proteolytic cleavage, the subunit containing the transmembrane domain in each case has an extremely hydrophobic region, termed the fusion peptide, or at near its newly generated N-terminus. In addition, all of these viral fusion proteins have 4-3 heptad repeat sequences near both the fusion peptide and the transmembrane domain. These regions have been demonstrated from a tight complex, in which the N-terminal heptad repeat forms a trimeric-coiled coil, with the C-terminal heptad repeat forming helical regions that buttress the coiled-coil in an anti-parallel manner. The significance of each of these structural elements in the processing and function of these viral fusion proteins is discussed.


Subject(s)
Membrane Fusion/physiology , Viral Fusion Proteins/chemistry , Viral Fusion Proteins/physiology , Protein Structure, Tertiary
4.
Mol Membr Biol ; 16(1): 11-9, 1999.
Article in English | MEDLINE | ID: mdl-10332733

ABSTRACT

The paramyxovirus fusion (F) protein mediates membrane fusion. The biologically active F protein consists of a membrane distal subunit F2 and a membrane anchored subunit F1. A highly stable structure has been identified comprised of peptides derived from the simian virus 5 (SV5) F1 heptad repeat A, which abuts the hydrophobic fusion peptide (peptide N-1), and the SV5 F1 heptad repeat B, located 270 residues downstream and adjacent to the transmembrane domain (peptides C-1 and C-2). In isolation, peptide N-1 is 47% alpha-helical and peptide C-1 and C-2 are unfolded. When mixed together, peptides N1 + C1 form a thermostable (Tm > 90 degrees C), 82% alpha-helical, discrete trimer of heterodimers (mass 31,300 M(r)) that is resistant to denaturation by 2% SDS at 40 degrees C. The authors suggest that this alpha-helical trimeric complex represents the core most stable form of the F protein that is either fusion competent or forms after fusion has occurred. Peptide C-1 is a potent inhibitor of both the lipid mixing and aqueous content mixing fusion activity of the SV5 F protein. In contrast, peptide N-1 inhibits cytoplasmic content mixing but not lipid mixing, leading to a stable hemifusion state. Thus, these peptides define functionally different steps in the fusion process. The parallels among both the fusion processes and the protein structures of paramyxovirus F proteins, HIV gp41 and influenza virus haemagglutinin are discussed, as the analogies are indicative of a conserved paradigm for fusion promotion among fusion proteins from widely disparate viruses.


Subject(s)
HIV Envelope Protein gp41/chemistry , Hemagglutinins, Viral/chemistry , Orthomyxoviridae/chemistry , Protein Conformation , Recombinant Fusion Proteins/chemistry , Respirovirus/chemistry , Dose-Response Relationship, Drug , Kinetics , Models, Biological , Temperature
5.
Mol Cell ; 3(3): 309-19, 1999 Mar.
Article in English | MEDLINE | ID: mdl-10198633

ABSTRACT

Paramyxoviruses are responsible for significant human mortality and disease worldwide, but the molecular mechanisms underlying their entry into host cells remain poorly understood. We have solved the crystal structure of a fragment of the simian parainfluenza virus 5 fusion protein (SV5 F), revealing a 96 A long coiled coil surrounded by three antiparallel helices. This structure places the fusion and transmembrane anchor of SV5 F in close proximity with a large intervening domain at the opposite end of the coiled coil. Six amino acids, potentially part of the fusion peptide, form a segment of the central coiled coil, suggesting that this structure extends into the membrane. Deletion mutants of SV5 F indicate that putative flexible tethers between the coiled coil and the viral membrane are dispensable for fusion. The lack of flexible tethers may couple a final conformational change in the F protein directly to the fusion of two bilayers.


Subject(s)
Membrane Fusion , Respirovirus/metabolism , Viral Fusion Proteins/chemistry , Amino Acid Sequence , Conserved Sequence , Crystallization , Dimerization , Electrons , Hydrogen Bonding , Ions , Models, Biological , Models, Molecular , Molecular Sequence Data , Peptide Fragments/chemistry , Peptide Fragments/genetics , Peptide Fragments/metabolism , Protein Binding , Protein Conformation , Protein Structure, Secondary , Respirovirus/chemistry , Respirovirus/genetics , Sequence Deletion , Viral Fusion Proteins/genetics , Viral Fusion Proteins/metabolism
6.
Virology ; 254(1): 147-59, 1999 Feb 01.
Article in English | MEDLINE | ID: mdl-9927582

ABSTRACT

The fusion (F) protein of the paramyxovirus SV5 contains two heptad repeat regions, HRA adjacent to the fusion peptide and HRB proximal to the transmembrane domain. Peptides, N-1 and C-1, respectively, corresponding to these heptad repeat regions form a thermostable, alpha-helical trimer of heterodimers (S. B. Joshi, R. E. Dutch, and R. A. Lamb (1998). Virology 248, 20-34). Further characterization of the N-1/C-1 complex indicated that the C-1 peptides, which are predicted to residue on the outside of the complex, are resistant to digestion by several proteases when present in the complex. Only proteinase K digested most of the C-1 peptide, though the small remaining protease protected fragment of C-1 confers extreme thermostability on the proteinase-K-resistant N-1 trimeric coiled-coil. Carboxypeptidase Y digestion of the N-1/C-1 complex indicates that the C-1 peptides associate in an antiparallel orientation relative to the N-1 peptides. Electron microscopy of the N-1/C-1 complex showed a rod-shaped complex with an average length of 9.7 nm, consistent with all of N-1 existing as an alpha helix. Mutations at heptad repeat a and d residues of N-1, positions that are predicted to point inward to the center of the N-1 trimeric coiled-coil, were found to have varying effects as analyzed by circular dichroism measurements. The mutation I137M did not affect the helical structure of the isolated N-1 peptide but did affect the thermostability of the N-1/C-1 complex. Mutations L140M and L161M perturbed the helical structure formed by N-1 in isolation but did not affect formation of a thermostable N-1/C-1 complex. Finally, a peptide, SV5 F 255-293, corresponding to a proposed leucine zipper region, was analyzed for effects on N-1, C-1, or the N-1/C-1 complex. Circular dichroism analysis demonstrated that while the presence of peptide 255-293 increased the helical signal from either N-1 or the N-1/C-1 complex, no change in thermostability was observed, indicating that this region is not a component of the final, most stable core of the F protein.


Subject(s)
Respirovirus/metabolism , Viral Fusion Proteins/metabolism , Amino Acid Sequence , Animals , Chymotrypsin , Leucine Zippers , Microscopy, Electron , Molecular Sequence Data , Mutation , Papain , Peptides , Respirovirus/ultrastructure , Temperature
7.
J Virol ; 72(10): 7745-53, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9733810

ABSTRACT

The membrane fusion reaction promoted by the paramyxovirus simian virus 5 (SV5) and human parainfluenza virus type 3 (HPIV-3) fusion (F) proteins and hemagglutinin-neuraminidase (HN) proteins was characterized when the surface densities of F and HN were varied. Using a quantitative content mixing assay, it was found that the extent of SV5 F-mediated fusion was dependent on the surface density of the SV5 F protein but independent of the density of SV5 HN protein, indicating that HN serves only a binding function in the reaction. However, the extent of HPIV-3 F protein promoted fusion reaction was found to be dependent on surface density of HPIV-3 HN protein, suggesting that the HPIV-3 HN protein is a direct participant in the fusion reaction. Analysis of the kinetics of lipid mixing demonstrated that both initial rates and final extents of fusion increased with rising SV5 F protein surface densities, suggesting that multiple fusion pores can be active during SV5 F protein-promoted membrane fusion. Initial rates and extent of lipid mixing were also found to increase with increasing influenza virus hemagglutinin protein surface density, suggesting parallels between the mechanism of fusion promoted by these two viral fusion proteins.


Subject(s)
HN Protein/metabolism , Hemagglutinin Glycoproteins, Influenza Virus/metabolism , Membrane Fusion , Respirovirus/metabolism , Viral Fusion Proteins/metabolism , Animals , Cell Line , Humans , Kinetics , Microscopy, Confocal
8.
Virology ; 248(1): 20-34, 1998 Aug 15.
Article in English | MEDLINE | ID: mdl-9705252

ABSTRACT

The paramyxovirus fusion (F) protein mediates membrane fusion. The biologically active F protein consists of a membrane distal subunit, F2, and a membrane-anchored subunit, F1. We have identified a highly stable structure composed of peptides derived from the F1 heptad repeat A, which abuts the hydrophobic fusion peptide (peptide N-1), and the F1 heptad repeat B, located 270 residues downstream and adjacent to the transmembrane domain (peptides C-1 and C-2). In isolation, peptide N-1 is 47% alpha-helical and peptide C-1 and C-2 are unfolded. When mixed together, peptides N1 + C1 form a thermostable (Tm >90 degreesC), 82% alpha-helical, discrete trimer of heterodimers (mass 31,300 Mr) that is resistant to denaturation by 2% SDS at 40 degreesC. We suggest that this alpha-helical trimeric complex represents the core most stable form of the F protein that either is fusion competent or forms after fusion has occurred. Peptide C-1 is a potent inhibitor of both the lipid mixing and the aqueous content mixing fusion activity of the SV5 F protein. In contrast, peptides N-1 and N-2 inhibit cytoplasmic content mixing but not lipid mixing, leading to a stable hemifusion state. Thus, these peptides define functionally different steps in the fusion process. The parallels among both the fusion processes and the protein structures of paramyxovirus F proteins, HIV gp41, and influenza virus hemagglutinin are discussed, as the analogies are indicative of a conserved paradigm for fusion promotion among fusion proteins from widely disparate viruses.


Subject(s)
HIV Envelope Protein gp41/chemistry , Hemagglutinin Glycoproteins, Influenza Virus/chemistry , Protein Structure, Secondary , Respirovirus/metabolism , Viral Fusion Proteins/biosynthesis , Viral Fusion Proteins/chemistry , Amino Acid Sequence , Animals , Cell Fusion , Cell Line , Circular Dichroism , Erythrocytes/physiology , HIV-1/metabolism , Humans , Macromolecular Substances , Models, Molecular , Molecular Sequence Data , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Sequence Alignment , Sequence Homology, Amino Acid , Thermodynamics
9.
Virology ; 239(2): 327-39, 1997 Dec 22.
Article in English | MEDLINE | ID: mdl-9434724

ABSTRACT

The paramyxovirus, simian virus 5, fusion (F) protein contains seven amino acids between heptad repeat B (a domain required for a biologically active fusion protein) and the presumptive boundary of the transmembrane (TM) domain. The role of the seven membrane proximal residues in stability and fusion promotion was examined by construction of a series of insertion, substitution, and deletion mutants, as manipulation of this region to enable proteolytic cleavage would facilitate production of a soluble F protein. The majority of the mutant F proteins both oligomerized and had kinetics of intracellular transport similar to those of wild-type (wt) F protein. All mutant F proteins were expressed at the cell surface at or near the same level as the wt F protein. However, by using both a qualitative lipid mixing assay and a quantitative content mixing assay for membrane fusion, it was found that mutant F proteins containing insertions in the region between heptad repeat B and the TM domain were unable to induce fusion, whereas the mutant F proteins containing substitutions in this region, together with three of the four mutants with deletions in this region, could induce fusion. Four of the F protein mutants contained a Factor Xa cleavage site, IEGR; however, Factor Xa treatment of cell surfaces released either none or only very small amounts (< 1% of total protein) of the soluble heterodimer F1 + F2. As an alternative method of generating soluble F protein, a glycosyl phosphatidylinositol (GPI) anchor was added to the F protein at three membrane-proximal positions. The highest level of surface expression was observed when the final molecule did not contain a significant insertion of amino acids into the membrane proximal region. Two F-GPI mutants reached the surface at approximately 20% of the levels seen with the wt F protein, and approximately 25% of the cell surface population of these mutants could be cleaved with phosphatidylinositol phospholipase C (PI-PLC) to yield soluble F protein. However, all the F-GPI mutants oligomerized aberrantly and failed to promote fusion. Taken together, these data indicate that the spacing of the region immediately adjacent to the presumptive boundary of the TM domain is extremely important for the fusogenic activity of the SV5 F protein.


Subject(s)
Membrane Fusion/physiology , Repetitive Sequences, Nucleic Acid , Respirovirus/physiology , Viral Fusion Proteins/physiology , Animals , Biological Transport , Cell Line , Chlorocebus aethiops , Cytopathogenic Effect, Viral , Factor Xa/metabolism , Glycosylphosphatidylinositols/metabolism , Membrane Lipids/metabolism , Mutagenesis, Site-Directed , Phosphatidylinositol Diacylglycerol-Lyase , Phosphoinositide Phospholipase C , Protein Conformation , Recombinant Fusion Proteins/metabolism , Respirovirus/chemistry , Respirovirus/genetics , Sequence Deletion , Solubility , Structure-Activity Relationship , Type C Phospholipases/metabolism , Viral Fusion Proteins/chemistry , Viral Fusion Proteins/genetics
10.
J Virol ; 69(5): 3084-9, 1995 May.
Article in English | MEDLINE | ID: mdl-7707536

ABSTRACT

Using an assay for recombination that measures deletion of a beta-galactosidase gene positioned between two directly repeated 350-bp sequences in plasmids transiently maintained in COS cells, we have found that replication from a simian virus 40 origin produces a high frequency of nonhomologous recombination. In contrast, plasmids replicating from a herpesvirus origin (oris) in COS cells superinfected with herpes simplex virus type 1 (HSV-1) show high levels of homologous recombination between the repeats and an enhanced recombinogenicity of the HSV-1 a sequence that is not seen during simian virus 40 replication. When the same assay was used to study recombination between 120- to 150-bp repeats in uninfected Vero cells, the level of recombination was extremely low or undetectable (< 0.03%), consistent with the fact that these repeats are smaller than the minimal efficient processing sequence for homologous recombination in mammalian cells. Recombination between these short repeats was easily measurable (0.5 to 0.8%) following HSV-1 infection, suggesting that there is an alteration of the recombination machinery. The frequency of recombination between repeats of the Uc-DR1 region, previously identified as the only segment of the HSV-1 a sequence indispensable for enhanced a-sequence recombination, was not significantly higher than that measured for other short sequences.


Subject(s)
DNA Replication/genetics , DNA, Viral/biosynthesis , DNA, Viral/genetics , Herpesvirus 1, Human/genetics , Herpesvirus 1, Human/physiology , Recombination, Genetic , Animals , Cell Transformation, Viral/genetics , Chlorocebus aethiops , Plasmids/genetics , Repetitive Sequences, Nucleic Acid , Replication Origin , Simian virus 40/genetics , Transfection , Vero Cells , Virus Replication/genetics , Virus Replication/physiology
11.
J Virol ; 68(6): 3733-41, 1994 Jun.
Article in English | MEDLINE | ID: mdl-8189511

ABSTRACT

The a sequences of herpes simplex virus type 1 are believed to be the cis sites for inversion events that generate four isomeric forms of the viral genome. Using an assay that measures deletion of a beta-galactosidase gene positioned between two directly repeated sequences in plasmids transiently maintained in Vero cells, we had found that the a sequence is more recombinogenic than another sequence of similar size. To investigate the basis for the enhanced recombination mediated by the a sequence, we examined plasmids containing direct repeats of approximately 350 bp from a variety of sources and with a wide range of G+C content. We observed that all of these plasmids show similar recombination frequencies (3 to 4%) in herpes simplex virus type 1-infected cells. However, recombination between directly repeated a sequences occurs at twice this frequency (6 to 10%). In addition, we find that insertion of a cleavage site for an a-sequence-specific endonuclease into the repeated sequences does not appreciably increase the frequency of recombination, indicating that the presence of endonuclease cleavage sites within the a sequence does not account for its recombinogenicity. Finally, by replacing segments of the a sequence with DNA fragments of similar length, we have determined that only the 95-bp Uc-DR1 segment is indispensable for high-level a-sequence-mediated recombination.


Subject(s)
Herpesvirus 1, Human/genetics , Recombination, Genetic , Animals , Base Sequence , DNA Replication/genetics , DNA, Viral/genetics , Endonucleases/genetics , Genome, Viral , Herpesvirus 1, Human/metabolism , Molecular Sequence Data , Repetitive Sequences, Nucleic Acid , Vero Cells
12.
J Virol ; 67(12): 6945-9, 1993 Dec.
Article in English | MEDLINE | ID: mdl-8230416

ABSTRACT

ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complementary single strands of DNA. This reaction is ATP independent but requires Mg2+. The activity is maximal at pH 7.6 and 80 mM NaCl. The major product of the reaction is double-stranded DNA, and no evidence of large DNA networks is seen. The reaction occurs at subsaturating concentrations of ICP8 but reaches maximal levels with saturating concentrations of ICP8. Finally, the renaturation reaction is second order with respect to DNA concentration. The ability of ICP8 to promote the renaturation of complementary single strands suggests a role for ICP8 in the high level of recombination seen in cells infected with herpes simplex virus type 1.


Subject(s)
DNA, Single-Stranded/metabolism , DNA, Viral/metabolism , Herpesvirus 1, Human/metabolism , Viral Proteins/pharmacology , Animals , Cells, Cultured , DNA-Binding Proteins , Dose-Response Relationship, Drug , Hot Temperature , Hydrogen-Ion Concentration , Magnesium/pharmacology , Moths/cytology , Nucleic Acid Renaturation/drug effects , Recombination, Genetic , Sodium Chloride/pharmacology
13.
Proc Natl Acad Sci U S A ; 89(22): 10950-4, 1992 Nov 15.
Article in English | MEDLINE | ID: mdl-1332062

ABSTRACT

We have partially purified an activity from extracts of cells infected with herpes simplex virus type 1 that mediates recombination between repeated copies of the 317-base-pair a sequence of herpes simplex virus type 1. Recombination leads to deletion of a lacZ indicator gene situated between two directly repeated copies of the a sequence and is scored by transformation of lacZ- Escherichia coli. The two products of the reaction can be observed directly by restriction enzyme digestion and Southern blot analysis. The recombinase activity is also detectable, but at a lower level, in uninfected cell extracts. The DNA substrate must contain the two a sequences arranged in direct orientation to generate the lacZ deletion. However, when the a sequences are arranged in inverted orientation, an inversion results. A substrate with two homologous sequences of size and G + C content similar to the a sequence undergoes recombination at a much lower frequency. The reaction requires a divalent cation (Mg2+ or Mn2+) but not ATP or any other nucleoside triphosphate. The simple requirements and specificity for the a sequence suggest that the recombination may proceed by a site-specific mechanism.


Subject(s)
DNA Nucleotidyltransferases/metabolism , Integrases , Recombination, Genetic , Simplexvirus/enzymology , Simplexvirus/genetics , Animals , Blotting, Southern , Cell Line , Chromatography, Affinity , Chromatography, Gel , DNA Nucleotidyltransferases/genetics , DNA, Viral/biosynthesis , DNA, Viral/genetics , DNA, Viral/isolation & purification , Escherichia coli/enzymology , Escherichia coli/genetics , Gene Deletion , Genes, Bacterial , Kinetics , Magnesium/pharmacology , Manganese/pharmacology , Osmolar Concentration , Recombinases , Restriction Mapping , beta-Galactosidase/genetics , beta-Galactosidase/metabolism
14.
J Virol ; 66(1): 277-85, 1992 Jan.
Article in English | MEDLINE | ID: mdl-1309247

ABSTRACT

During the course of infection, elements of the herpes simplex virus type 1 (HSV-1) genome undergo inversion, a process that is believed to occur through the viral a sequences. To investigate the mechanism of this recombinational event, we have developed an assay that detects the deletion of DNA segments flanked by directly repeated a sequences in plasmids transiently maintained in Vero cells. With this assay, we have observed a high frequency of recombination (approximately 8%) in plasmids that undergo replication in HSV-1-infected cells. We also found a low level of recombination between a sequences in plasmids introduced into uninfected cells and in unreplicated plasmids in HSV-1-infected cells. In replicating plasmids, recombination between a sequences occurs at twice the frequency seen with directly repeated copies of a different sequence of similar size. Recombination between a sequences appears to occur at approximately the same time as replication, suggesting that the processes of replication and recombination are closely linked.


Subject(s)
DNA Replication , DNA, Viral , Recombination, Genetic , Repetitive Sequences, Nucleic Acid , Simplexvirus/genetics , Animals , Base Sequence , Cloning, Molecular , DNA, Viral/biosynthesis , DNA, Viral/genetics , Kinetics , Plasmids , Restriction Mapping , Vero Cells
15.
J Virol ; 65(3): 1649-52, 1991 Mar.
Article in English | MEDLINE | ID: mdl-1847482

ABSTRACT

The herpes simplex virus type 1 genome contains three origins of replication: OriL and a diploid OriS. The origin-binding protein, the product of the UL9 gene, interacts with two sites within OriS, box I and box II. A third site, box III, which is homologous to boxes I and II, may also be a binding site for the origin-binding protein. Mutations in these three sites significantly reduce OriS-directed plasmid replication measured in transient replication assays. The reduction in replication efficiency of the mutants correlates well with the decrease in the ability to bind to the origin-binding protein, as determined by Elias et al. (P. Elias, C. M. Gustafsson, and O. Hammarsten, J. Biol. Chem. 265: 17167-17173, 1990). The effect of multiple mutations in boxes I, II, and III on plasmid replication suggests that there are multiple binding sites in OriS for the origin-binding protein. These studies indicate that proper interaction of the origin-binding protein with the OriS sequence is essential for OriS-directed DNA replication.


Subject(s)
DNA-Binding Proteins/genetics , Mutagenesis, Site-Directed , Simplexvirus/genetics , Viral Proteins/genetics , Animals , Base Sequence , Cell Line , Cloning, Molecular , DNA-Binding Proteins/metabolism , Molecular Sequence Data , Plasmids , Restriction Mapping , Sequence Homology, Nucleic Acid , Transfection , Vero Cells , Viral Proteins/metabolism
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