[Activity of pollulan synthesis by diploid culture of Pollularia pollulans on media with different sources of carbon]. / Aktivnost' sinteza pullulana diploidnoi kul'turoi Pullularia pullulans na sredakh s razlichnymi istochnikami ugleroda.

The diploid culture of Pullularia pullulans 1125(4)(13) synthesizes pullulan from different carbon sources. The activity is highest on a mineral medium with melassa (23.18 g/L). Pullulan is accumulated in great effectiveness on a medium with corn flour hydrolysate (48.23% of the carbon source amount).

[Carbohydrates of the medium and the nature of ontogeny in Aspergillus awamori in relation to glucoamylase biosynthesis]. / Uglevody sredy i kharakter ontogeneza u Aspergillus awamori v sviazi s biosintezom gliukoamilazy.

The biological rhythm of the transition from the processes of blocking and nuclear division to active RNA biosynthesis and growth in submerged culture of Aspergillus awamori producing glucoamylase was studied by the technique of quantitative cytochemistry, and was characterized by distinct daily oscillation in a medium with starch. The rhythm was disturbed when other carbon sources, viz. maltose, sucrose and particularly glucose, were used: the rhythm became longer and uneven, the rate of nuclear blocking decreased, and the de novo formation of the mycelium of the adaptive stage capable of producing glucoamylase was inhibited. As we have shown earlier, such rhythms in fungi are induced at the level of nuclear processes; in this study, they were most affected by the disturbances which led to the inhibition of the enzyme production. It is quite possible therefore that the processes of biochemical adaptation in fungal ontogenesis, particulaly the production of hydrolytic enzymes, are regulated at the molecular-biological level in the initiation of their synthesis rather than at the level of competition between the existing pathways of general metabolism, and are closely related to biorhythms.

[Study of the conditions maintaining the synthesis of alpha-amylase by Bacillus species]. / Izuchenie uslovii, obespechivaiushchikh obrazovanie alpha-amilazy Bacillus species

The effect of temperature and different carbon sources on the growth of Bacillus species and the synthesis of alpha-amylase was studied. The temperature of 40 degrees proved to be optimal for those processes. Starch was found to be the best carbon source. The maximum accumulation of the enzyme was seen by the 40th hour of growth.