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1.
Zh Mikrobiol Epidemiol Immunobiol ; (10): 45-8, 1990 Oct.
Article in Russian | MEDLINE | ID: mdl-2075765

ABSTRACT

57 strains of different microbial species, isolated from patients having complicated forms of acute cholecystitis, were studied with the use of the reaction of bacteriosorption on immobilized proteins: fibrinogen, fibronectin, gamma globulin, ovalbumin, etc. The reaction of bacteriosorption was positive with all 4 Staphylococcus aureus strains: they were adsorbed on fibrinogen, fibronectin and gamma globulin. Besides, 4 out of 18 Staphylococcus epidermidis strains were selectively adsorbed on fibronectin and 1 of these strains was adsorbed on ovalbumin, while 2 out of 15 Escherichia coli strains were adsorbed on ovalbumin. S. epidermidis strains studied in this work differed from 15 strains of the same species, isolated from the urine of patients and studied earlier, by their adsorption properties.


Subject(s)
Bacteria/metabolism , Cholecystitis/microbiology , Proteins/metabolism , Abdomen/microbiology , Acute Disease , Adsorption , Bacteria/isolation & purification , Bile/microbiology , Cholecystitis/complications , Exudates and Transudates/microbiology , Gallbladder/microbiology , Humans , Protein Binding , Solubility
2.
Antibiot Khimioter ; 35(2): 46-8, 1990 Feb.
Article in Russian | MEDLINE | ID: mdl-2186711

ABSTRACT

Investigation of microflora in patients with complicated acute cholecystitis treated with low frequency ultrasound in combination with sulfacrylate glue showed that Staphylococcus and Escherichia played the leading role in the complication etiology. Among the nonsporulating organisms bacteroides predominated. The combined use of low frequency ultrasound and sulfacrylate glue in treatment of complicated acute cholecystitis proved to be an efficient procedure providing more rapid sanation of the purulent inflammation foci.


Subject(s)
Acrylic Resins/therapeutic use , Cholecystectomy/methods , Cholecystitis/microbiology , Escherichia coli Infections/microbiology , Gallbladder/microbiology , Staphylococcal Infections/microbiology , Tissue Adhesives/therapeutic use , Ultrasonic Therapy , Acute Disease , Adult , Cholecystitis/surgery , Colony Count, Microbial , Escherichia coli/isolation & purification , Escherichia coli Infections/surgery , Female , Gallbladder/surgery , Humans , Intraoperative Care , Male , Middle Aged , Staphylococcal Infections/surgery , Staphylococcus/isolation & purification
3.
Article in Russian | MEDLINE | ID: mdl-3929502

ABSTRACT

The protein composition of Mycobacterium tuberculosis and the molecular weight of proteins contained in these organisms have been determined by the method of electrophoresis in the porosity gradient of polyacrylamide gel. Close similarity between the electrophoregrams of M. tuberculosis clinical and laboratory strains has been revealed. The study of 18 strains of different groups of mycobacteria has shown that M. tuberculosis are essentially different from opportunistic mycobacteria and acid-resistant saprophytes. These data may be important for the identification and taxonomy of mycobacteria.


Subject(s)
Bacterial Proteins/analysis , Mycobacterium/analysis , Electrophoresis, Polyacrylamide Gel/methods , Molecular Weight , Mycobacterium/classification , Mycobacterium phlei/analysis , Mycobacterium phlei/classification , Mycobacterium tuberculosis/analysis , Mycobacterium tuberculosis/classification , Nontuberculous Mycobacteria/analysis , Nontuberculous Mycobacteria/classification
11.
Antibiotiki ; 21(11): 988-92, 1976 Nov.
Article in Russian | MEDLINE | ID: mdl-798545

ABSTRACT

Antibiotic sensitivity of the pharynx mucosa microflora in patients with chronic pharyngitis subjected to cryotherapy was studied. Examination of 36 patients showed that cryotherapy at a temperature of -1950 markedly decreased the number of the grown colonies, their qualitative characteristics being changed. In 10 days both the quantitative and the qualitative characteristics of the flora completely reduced. The cryotherapy did not change the virulence of the flora and its sensitivity to antibiotics.


Subject(s)
Anti-Bacterial Agents/pharmacology , Cryosurgery , Pharyngitis/microbiology , Staphylococcus/drug effects , Streptococcus/drug effects , Chronic Disease , Humans , Microbial Sensitivity Tests , Pharyngitis/therapy , Streptococcus agalactiae/drug effects , Time Factors
15.
Zh Mikrobiol Epidemiol Immunobiol ; (1): 108-13, 1975 Jan.
Article in Russian | MEDLINE | ID: mdl-804775

ABSTRACT

A study of proteinograms of some strains of mycobacteria with the use of the method of electrophoresis in polyacrylamide gel showed that individual strains had characteristic protein spectra which could serve as one of the possible taxonomic characteristics. Analysis of proteinograms of three groups of mycobacteria (tuberculosis, atypical and saprophytes) by electrophoretic mobility of the fractions, with consideration to their homologous character and by the intensity of protein fractions in the mobility zones pointed to heterogeneity of the group of atypical strains and their greater similarity to tuberculosis mycobacteria than to acid-fast saprophytes. Proteinograms of strains H37 Rv and Bov-8 proved to be identical; a marked similarity between the protein spectra of M. avium and M. battey was revealed.


Subject(s)
Bacterial Proteins/analysis , Mycobacterium/analysis , Electrophoresis, Polyacrylamide Gel , Mycobacterium bovis/analysis , Mycobacterium tuberculosis/analysis
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