ABSTRACT
Genetic variability and genetic differentiation in two Ovis species--domesticated (Askanian sheep, Sokilska and Kulunda sheep) and wild (bighorn sheep) were analyzed using different types of molecular-genetic markers--genetic-biochemical (30 loci) and DNA (ISSR-PCR) ones. High level of genetic variability was revealed. The average heterozygosity of biochemical markers loci was in range of 0.073-0.188. Markers involved into process of genofond divergence of two closely related species were revealed.
Subject(s)
DNA/genetics , Sheep/genetics , Animals , Genetic Markers , Genetic Variation , Polymerase Chain Reaction , Species SpecificityABSTRACT
The comparative analysis of genetic differentiations between three species of Bovinae--Bos taurus, Bison bonasus, Bison bison with the use of different types of molecular-genetic markers--genetical-biochemical (35 loci) and DNA markers (RAPD-PCR, ISSR-PCR) was carried out. It was shown, that the evaluation of interspecies genetic interrelations was connected more with the determined molecular-genetic markers (loci), included in analysis, than with the marker's belonging to certain type (protein polymorphism, variability of DNA repeat distributions).
Subject(s)
Bison/genetics , Blood Proteins/genetics , Cattle/genetics , Polymorphism, Genetic/genetics , Alleles , Animals , Bison/blood , Cattle/blood , Electrophoresis, Polyacrylamide Gel , Electrophoresis, Starch Gel , Genetic Markers/genetics , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/statistics & numerical data , Random Amplified Polymorphic DNA Technique , Repetitive Sequences, Nucleic Acid , Species SpecificityABSTRACT
The literature data on milk protein (particularly kappa-casein) polymorphism study in different cattle breeds are represented. Allele variants of kappa-kasein are associated with lactation and have an influence on the composition of milk and its properties, including cheese-making properties. The importance of ways of kappa-casein genetic variants use in concrete selection programs are discussed.