ABSTRACT
A comparative study was made of the effect of X-radiation on the membrane-bound glucoso-6-phosphatase of the nuclear membrane and microsomal fraction of calf thymus cells. Dose- and concentration-dependencies of inactivation of glucoso-6-phosphatase are indicative of a higher radiosensitivity of glucoso-6-phosphatase of nuclear membranes than that of microsomes. This difference in radiosensitivity is associated with the peculiarities of the composition and structural organization of these two membrane systems of a cell.
Subject(s)
Glucose-6-Phosphatase/radiation effects , Intracellular Membranes/metabolism , Animals , Cattle , Cell Nucleus/metabolism , Glucose-6-Phosphatase/metabolism , In Vitro Techniques , Microsomes/metabolism , Radiation Tolerance , Thymus Gland/ultrastructureABSTRACT
A study of dioxydine effect on the membrane apparatus of Staphylococcus aureus 209 P and Micrococcus lysodeikticus revealed a decrease in the dehydrogenase activity of membrane preparations and in the specific concentration of cytochromes, if cells had been cultivated in a medium with subbacteriostatic concentrations of dioxydine. The total activity of the respiratory chain was slightly decreased. Conservatism of the membrane apparatus of the bacteria studied was demonstrated.
Subject(s)
Anti-Bacterial Agents/pharmacology , Micrococcus/drug effects , Quinoxalines/pharmacology , Staphylococcus aureus/drug effects , Cell Membrane/drug effects , Cell Membrane/enzymology , Cytochromes/metabolism , Micrococcus/enzymology , Oxidoreductases/metabolism , Protoplasts/drug effects , Protoplasts/enzymology , Staphylococcus aureus/enzymologyABSTRACT
The antimicrobial properties of two cationic detergents, i.e. rockal, an antiseptic made in England and catamine AB, its analog made in the USSR were compared. It was found that catamine AB and rockal did not practically differ by their bacteriostatic and mycostatic activities with respect to collection and clinical strains of bacteria with multiple resistance to antibiotics. Both detergents were shown to be identical by their membranotropic activity and thus catamine AB may be used instead of rockal as an antiseptic.
Subject(s)
Anti-Infective Agents, Local/pharmacology , Bacteria/drug effects , Quaternary Ammonium Compounds/pharmacology , Surface-Active Agents/pharmacology , Bacteria/ultrastructure , Cell Membrane/drug effects , Fungi/drug effects , Fungi/ultrastructure , Kinetics , Microbial Sensitivity Tests , Time FactorsABSTRACT
Three methods of decomposition of Staphylococcus aureus 209-P cells were compared for the production of membranes-ultrasound, a press, and enzymatic lysis. All the tested methods permitted the isolation of enzymatically active membranes, differing somewhat in protein composition.
Subject(s)
Bacterial Proteins/analysis , Membrane Proteins/analysis , Staphylococcus aureus/analysis , Cell Membrane/analysis , Cell Membrane/enzymology , Cell Membrane/ultrastructure , Cytochromes/analysis , Oxidoreductases/analysis , Staphylococcus aureus/ultrastructureABSTRACT
Certain new nonionogenic detergents which are hydroxy-polyethoxy-derivatives of dodecane show in vitro bacteriostatic action as related to gram-positive microorganisms. In this respect they are not inferior to chloride cetylpyridinium or sodium dodecyl sulphate. They are similar to chloride cetylpyridinium in their ability to disrupt the osmotic barrier of protoplasts and to inhibit NAD-H-oxidase of membrane fractions of Micrococcus lysodeikticus. Unlike chloride cetylpyridinium and sodium dodecyl sulphate, nonionogenic detergents do not inhibit membrane-bound ATPase of these bacteria.
