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1.
Integr Org Biol ; 4(1): obac029, 2022.
Article in English | MEDLINE | ID: mdl-36034057

ABSTRACT

The adaptive significance of colorful or exaggerated traits (i.e., ornaments) expressed in females is often unclear. Competing hypotheses suggest that expression of female ornaments arises from maladaptive (or neutral) genetic inheritance from males along with incomplete epigenetic regulation, or from positive selection for ornaments in females under social competition. Whether costly or advantageous, the visibility of such traits can sometimes be behaviorally modulated in order to maximize fitness. Female eastern fence lizards express blue badges that are variable in size and color saturation. These are rudimentary compared to those seen in males and carry important costs such as reduced mating opportunities. Body temperature is a well-established enhancer of badge color, and thus thermoregulation may be one way these animals modulate badge visibility. We quantified realized body temperatures of female lizards paired in laboratory trials and observed that females with larger badges attained higher body temperatures when freely allowed to thermoregulate, sometimes beyond physiological optima. In this association between phenotype and behavior, females with larger badges exhibited thermoregulatory patterns that increase their badges' visibility. This signal-enhancing behavior is difficult to reconcile with the widely held view that female ornaments are maladaptive, suggesting they may carry context-dependent social benefits.


É muitas vezes incerta a significância adaptativa de caracteres vívidos e coloridos em fêmeas. Hipóteses para esse fenômeno sugerem uma herança maladaptativa (ou neutra) de caracteres selecionados em machos aliada à regulação epigenética incompleta em fêmeas, ou ainda seleção positiva em fêmeas imposta por competição social. Vantajosos ou não, a visibilidade de ornamentos muitas vezes é modulada por vias comportamentais do portador de modo a balancear seus custos e benefícios. Fêmeas Sceloporus undulatus possuem um par de marcas verde-azuis na parte ventral do pescoço que são variáveis em área e saturação entre indivíduos. Esses ornamentos são rudimentares em relação aos vistos em machos, mas ainda assim estão associados a custos reprodutivos importantes. Nessa espécie, temperatura corporal aumenta a visibilidade desses ornamentos significativamente. Portanto, a termorregulação é um comportamento que fêmeas poderiam empregar para modular a visibilidade de ornamentos. Nós quantificamos temperaturas corporais obtidas por pares de lagartos fêmeas em testes comportamentais e observamos que fêmeas com os maiores ornamentos também obtiveram temperaturas corporais mais altas, às vezes além do ótimo fisiológico. Nesta associação entre fenótipo e comportamento, fêmeas com os maiores ornamentos exibiram padrões de termoregulação que aumentaram sua visibilidade. Este padrão é difícil de conciliar com a perspectiva de que ornamentos são maladaptativos em fêmeas, sugerindo benefícios que são dependentes do contexto social.

2.
Gen Comp Endocrinol ; 270: 113-122, 2019 01 01.
Article in English | MEDLINE | ID: mdl-30339807

ABSTRACT

The vertebrate stress response enables individuals to react to and cope with environmental challenges. A crucial aspect of the stress response is the elevation of circulating glucocorticoids. However, continued activation of the stress response under repeated exposure to stressors can be damaging to fitness. Under certain circumstances it may therefore be adaptive to habituate to repeated exposures to a particular stressor by reducing the magnitude of any associated release of glucocorticoids. Here, we investigate whether Trinidadian guppies (Poecilia reticulata) habituate to repeated exposure to a mild stressor, using a waterborne hormone sampling approach that has previously been shown to elicit a stress response in small fish. We also test for individual variation in the extent of habituation to this stressor. Concentrating on freely circulating cortisol, we found that the first exposure to the assay induced high cortisol release rates but that guppies tended to habituate quickly to subsequent exposures. There were consistent differences among individuals in their average cortisol release rate (after accounting for effects of variables such as body size) over repeated exposures. Our analyses did not find evidence of individual differences in habituation rate, although limitations in statistical power could account for this finding. We repeated the analysis for free 11-ketotestosterone, which can also respond to stressors, but found no obvious habituation pattern and no among-individual variation. We also present data on conjugated forms of both hormones, which were repeatable but did not show the expected time-lagged habituation effect. We discuss consistent individual differences around the general pattern of habituation in the flexible stress response, and highlight the potential for individual variation in habituation to facilitate selection against the deleterious effects of chronic stress.


Subject(s)
Hydrocortisone/metabolism , Substance-Related Disorders/metabolism , Animals , Hydrocortisone/analysis , Individuality , Male , Poecilia
3.
R Soc Open Sci ; 5(3): 172268, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29657818

ABSTRACT

To survive, animals must respond appropriately to stress. Stress responses are costly, so early-life experiences with potential stressors could adaptively tailor adult stress responses to local conditions. However, how multiple stressors influence the development of the stress response remains unclear, as is the role of sex. Trinidadian guppies (Poecilia reticulata) are small fish with extensive life-history differences between the sexes and population variation in predation pressure and social density. We investigated how sex and early-life experience influence hormonal stress responses by manipulating conspecific density and perceived predation risk during development. In adults, we sampled cortisol twice to measure initial release and change over time in response to a recurring stressor. The sexes differed considerably in their physiological stress response. Males released more cortisol for their body mass than females and did not reduce cortisol release over time. By contrast, all females, except those reared at high density together with predation cues, reduced cortisol release over time. Cortisol responses of males were thus less dynamic in response to current circumstances and early-life experiences than females, consistent with life-history differences between the sexes. Our study underscores the importance of early-life experiences, interacting ecological factors and sex differences in the organization of the stress response.

4.
J Exp Biol ; 221(Pt 8)2018 04 13.
Article in English | MEDLINE | ID: mdl-29511069

ABSTRACT

Fishes acclimated to hypoxic environments often increase gill surface area to improve O2 uptake. In some species, surface area is increased via reduction of an interlamellar cell mass (ILCM) that fills water channels between gill lamellae. Amphibious fishes, however, may not increase gill surface area in hypoxic water because these species can, instead, leave water and breathe air. To differentiate between these possibilities, we compared wild amphibious mangrove rivulus Kryptolebias marmoratus from two habitats that varied in O2 availability - a hypoxic freshwater pool versus nearly anoxic crab burrows. Fish captured from crab burrows had less gill surface area (as ILCMs were enlarged by ∼32%), increased rates of normoxic O2 consumption and increased critical O2 tension compared with fish from the freshwater pool. Thus, wild mangrove rivulus do not respond to near-anoxic water by decreasing metabolism or increasing O2 extraction. Instead, fish from the crab burrow habitat spent three times longer out of water, which probably caused the observed changes in gill morphology and respiratory phenotype. We also tested whether critical O2 tension is influenced by genetic heterozygosity, as K. marmoratus is one of only two hermaphroditic vertebrate species that can produce both self-fertilized (inbred) or out-crossed (more heterozygous) offspring. We found no evidence for inbreeding depression, suggesting that self-fertilization does not impair respiratory function. Overall, our results demonstrate that amphibious fishes that inhabit hypoxic aquatic habitats can use a fundamentally different strategy from that used by fully aquatic water-breathing fishes, relying on escape behaviour rather than metabolic depression or increased O2 extraction ability.


Subject(s)
Cyprinodontiformes/anatomy & histology , Cyprinodontiformes/physiology , Gills/anatomy & histology , Gills/physiology , Oxygen/analysis , Animals , Cyprinodontiformes/genetics , Ecosystem , Fresh Water/chemistry , Hermaphroditic Organisms , Inbreeding Depression , Oxygen Consumption
5.
J Fish Biol ; 88(3): 1125-42, 2016 Mar.
Article in English | MEDLINE | ID: mdl-26806153

ABSTRACT

This study validated a technique for non-invasive hormone measurements in California killifish Fundulus parvipinnis, and looked for associations between cortisol (a stress hormone) and 11-ketotestosterone (KT, an androgen) release rates and the density or intensity of the trematode parasites Euhaplorchis californiensis (EUHA) and Renicola buchanani (RENB) in wild-caught, naturally infected F. parvipinnis. In experiment 1, F. parvipinnis were exposed to an acute stressor by lowering water levels to dorsal-fin height and repeatedly handling the fish over the course of an hour. Neither parasite was found to influence cortisol release rates in response to this acute stressor. In experiment 2, different F. parvipinnis were exposed on four consecutive days to the procedure for collecting water-borne hormone levels and release rates of 11-KT and cortisol were quantified. This design examined whether F. parvipinnis perceived the water-borne collection procedure to be a stressor, while also exploring how parasites influenced hormone release rates under conditions less stressful than those in experiment 1. No association was found between RENB and hormone release rates, or between EUHA and 11-KT release rates. The interaction between EUHA density and handling time, however, was an important predictor of cortisol release rates. The relationship between handling time and cortisol release rates was negative for F. parvipinnis harbouring low or intermediate density infections, and became positive for fish harbouring high densities of EUHA.


Subject(s)
Brain/parasitology , Fish Diseases/physiopathology , Handling, Psychological , Hydrocortisone/metabolism , Killifishes , Testosterone/analogs & derivatives , Trematode Infections/veterinary , Animals , Blood Chemical Analysis/standards , California , Killifishes/parasitology , Killifishes/physiology , Parasite Load , Reproducibility of Results , Testosterone/metabolism , Time Factors , Trematoda/physiology , Trematode Infections/physiopathology
6.
J Fish Biol ; 88(2): 774-86, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26563824

ABSTRACT

Kryptolebias marmoratus exposed to 4 ng l(-1) of ethinyl oestradiol (EE2) for 30 days experienced significant changes in endogenous 17ß-oestradiol (E2) and 11-ketotestosterone (KT) and qualitative changes in gonad morphology. Both hermaphrodites and males showed a significant decrease in E2, whereas only males exhibited a significant decrease in KT. Exposure to EE2 resulted in a decrease in spermatid and spermatocyte density in males and an increase in the number of early stage oocytes in hermaphrodites.


Subject(s)
Endocrine Disruptors/toxicity , Ethinyl Estradiol/toxicity , Gonads/drug effects , Killifishes/physiology , Reproduction/drug effects , Animals , Environment , Estradiol/blood , Female , Hermaphroditic Organisms/physiology , Male , Oocytes/drug effects , Spermatids/drug effects , Spermatocytes/drug effects , Testosterone/analogs & derivatives , Testosterone/blood
7.
Heredity (Edinb) ; 115(4): 335-48, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26243135

ABSTRACT

Phenotypic plasticity can influence evolutionary change in a lineage, ranging from facilitation of population persistence in a novel environment to directing the patterns of evolutionary change. As the specific nature of plasticity can impact evolutionary consequences, it is essential to consider how plasticity is manifested if we are to understand the contribution of plasticity to phenotypic evolution. Most morphological traits are developmentally plastic, irreversible, and generally considered to be costly, at least when the resultant phenotype is mis-matched to the environment. At the other extreme, behavioral phenotypes are typically activational (modifiable on very short time scales), and not immediately costly as they are produced by constitutive neural networks. Although patterns of morphological and behavioral plasticity are often compared, patterns of plasticity of life history phenotypes are rarely considered. Here we review patterns of plasticity in these trait categories within and among populations, comprising the adaptive radiation of the threespine stickleback fish Gasterosteus aculeatus. We immediately found it necessary to consider the possibility of iterated development, the concept that behavioral and life history trajectories can be repeatedly reset on activational (usually behavior) or developmental (usually life history) time frames, offering fine tuning of the response to environmental context. Morphology in stickleback is primarily reset only in that developmental trajectories can be altered as environments change over the course of development. As anticipated, the boundaries between the trait categories are not clear and are likely to be linked by shared, underlying physiological and genetic systems.


Subject(s)
Adaptation, Biological/genetics , Biological Evolution , Phenotype , Smegmamorpha/genetics , Animals , Behavior, Animal , Environment , Female , Reproduction , Smegmamorpha/anatomy & histology , Smegmamorpha/physiology
8.
Gen Comp Endocrinol ; 155(2): 438-46, 2008 Jan 15.
Article in English | MEDLINE | ID: mdl-17869251

ABSTRACT

The primary goals of this study were to evaluate diurnal patterns of and sex differences in the levels of cortisol, 11-ketotestosterone, testosterone, and 17beta-estradiol in the sex-changing bluebanded goby Lythrypnus dalli. Steroid hormones were collected from water samples and analyzed by enzyme immunoassay. During the breeding season, hormones were sampled from both males and females at seven time points between 0600 and 2000 h. When comparing each time point separately, there were significant overall time effects for cortisol and 17beta-estradiol. Cortisol concentrations were lowest at the 0800-1000 h sampling point and showed a qualitative peak in late morning (1000-1200 h). Concentrations of 17beta-estradiol were elevated at the last sampling point (1800-2000 h). Broader temporal trends were revealed for testosterone and 11-ketotestosterone concentrations, both of which were elevated in the morning. There were no sex differences in overall hormone concentrations or temporal profiles for cortisol, 11-ketotestosterone, or testosterone. Males and females showed similar diurnal patterns of 17beta-estradiol but females had significantly higher water-borne 17beta-estradiol levels than males. The results show the presence of diurnal changes in steroid hormone levels in male and female bluebanded gobies. The lack of sex differences in androgens suggests that males of this species, and perhaps other bi-directional sex-changing species in which males do not exhibit prominent secondary sexual characteristics, do not require persistent elevations in 11-ketotestosterone or testosterone to maintain the male phenotype. Although the role of 17beta-estradiol in maintaining sex differences in sexually plastic species is unclear, our results suggest that, of the hormones measured, 17beta-estradiol has the greatest potential for future studies interested in this question.


Subject(s)
Circadian Rhythm/physiology , Estradiol/metabolism , Hydrocortisone/metabolism , Perciformes/metabolism , Testosterone/analogs & derivatives , Testosterone/metabolism , Analysis of Variance , Animals , Female , Male , Sex Factors
9.
Behav Processes ; 73(3): 290-8, 2006 Nov 01.
Article in English | MEDLINE | ID: mdl-16919400

ABSTRACT

When the same set of individuals are placed in different social contexts, some groups members often experience a change in dominance status. We examined the context-dependence of social status using a group fusion protocol in male green swordtail fish (Xiphophorus helleri). Six individuals were matched for size and separated into two groups of three fish. Each triad established a stable hierarchy after which time the two subgroups were merged into one larger assemblage. The maintenance of within- and between-group rank relationships was examined. Relative rank was preserved within each subgroup across social contexts but we found no evidence that familiarity with dominant animals assists individuals of one subgroup in achieving higher rank (coat-tail effects). Dominant individuals from the pre-fusion groups were significantly likely to obtain high status in the merged group and vice versa for subordinate pre-fusion animals. These results demonstrate that social rank in swordtails is relatively impervious to changes in social context, but we address some deviations from this trend. Small differences in standard length were a significant predictor of the most dominant rank in the post-fusion hierarchy, with the largest animals tending to occupy the alpha position. We discuss our results in terms of the potential factors involved in within- and between-group rank maintenance, including individual recognition, winner and loser effects, or asymmetries in dominance-related characteristics.


Subject(s)
Hierarchy, Social , Social Dominance , Aggression , Animals , Cyprinodontiformes , Group Processes , Male , Social Environment , Statistics, Nonparametric
10.
Horm Behav ; 49(5): 610-4, 2006 May.
Article in English | MEDLINE | ID: mdl-16524575

ABSTRACT

The relationship between androgens and paternal behavior is not straightforward, potentially because of the diversity of tasks a male must undertake to maximize reproductive success, notably alternating between courtship, aggression, and offspring care. In some species, these events are separated in time, but in others they are coincident. The endocrine profiles of species that simultaneously court, parent, and defend a nest, such as male bluebanded gobies (Lythrypnus dalli), are not well understood. We sampled a potent fish androgen, 11-ketotestosterone (KT), at different life history stages (experienced parenting males, experienced males not actively parenting, inexperienced males with their first clutch, and females), to examine this relationship. We found that experienced parenting L. dalli males have the highest KT levels of any group, while none of the other groups differed significantly. Males showed elevated KT levels when they had eggs compared to when they did not. Our data suggest that KT facilitates at least some aspects of parental care in L. dalli.


Subject(s)
Hermaphroditic Organisms , Nesting Behavior/physiology , Perciformes/physiology , Sex Determination Processes , Sexual Behavior, Animal/physiology , Testosterone/analogs & derivatives , Animals , Female , Larva/growth & development , Male , Testosterone/blood
11.
Talanta ; 45(6): 1255-66, 1998 Apr.
Article in English | MEDLINE | ID: mdl-18967118

ABSTRACT

A mixture of seven penicillins was separated on a C-8 column and detected using pulsed amperometric detection (PAD). Due to the polarity range of the penicillin mixture, a gradient program was necessary to produce a reasonable separation, causing some baseline shifting. Application of a flow program, where the solvent flow rate is varied within the separation, was also examined, and found to cause only a small shift in baseline response for PAD. Further examination of a variety of different flow programs was undertaken to characterize the baseline and analyte response under these conditions. Notable was that the shift in baseline from the flow program could be altered to give a shift toward either increased or decreased current, depending on the waveform and solvent combination in use. By manipulating these two parameters, one can customize the PAD response to a flow program, which may allow flow programs to be designed that only produce a minimal impact on the detector response.

12.
Talanta ; 46(6): 1507-14, 1998 Aug.
Article in English | MEDLINE | ID: mdl-18967281

ABSTRACT

An examination was made of the effect of electrode polishing on the direct pulsed amperometric detection (PAD) and indirect PAD response to penicillin G. The polishing procedure produced a temporary loss of sensitivity for both types of PAD, which over time relaxed back to higher levels. A temporary increase in the electrode kinetics can be observed as well, particularly for a PAD waveform in the crossover region between direct and indirect PAD. In extreme cases, the penicillin G response can undergo a complete polarity reversal. By observing post-polish equilibration times, it was recommended that the direct and indirect PAD user observe 3 and 5 h delay times, respectively, to insure stable electrode response following an electrode polish.

13.
Talanta ; 42(1): 17-25, 1995 Jan.
Article in English | MEDLINE | ID: mdl-18966195

ABSTRACT

Aided by the construction of a custom potentiostat, a series of different PAD waveforms were compared to find the optimum detector for penicillin oxidation. The waveforms included standard 3-step direct and indirect PAD in addition to reverse-PAD and 4-step PAD. Two new waveforms, the indirect reverse-PAD and the 4-step indirect PAD were examined in the study. Under the solvent conditions of the study (0.01M acetate buffer, pH 4.6) the indirect waveforms yielded the best detectability for penicillin G while the reverse-PAD waveforms yielded the worst performance. The 4-step PAD methods did not improve detectability when compared to the 3-step types, but they did provide output peak profiles with better shapes and less tailing. Although indirect waveforms gave better detectability than direct detection in the 0.01M acetate buffer solution, the limits of detection for each were found to be differing functions of ionic strength. At higher acetate concentrations, direct PAD was more favorable than indirect detection.

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