ABSTRACT
A 'one tube' reverse transcription-polymerase chain reaction ('one tube' RT-PCR) using rTth DNA polymerase was compared with an existing RT-PCR using Taq DNA polymerase (Taq RT-PCR) to detect ruminant pestiviruses in infected cell cultures. The technically simpler and more convenient 'one tube' method was relatively insensitive detecting only 11 of the 34 samples tested, all of which were positive by Taq RT-PCR.
Subject(s)
Pestivirus/isolation & purification , Polymerase Chain Reaction/methods , Animals , Cells, Cultured , Pestivirus/genetics , RuminantsABSTRACT
We report two cases of villous adenoma of the duodenum, one arising from the main papilla and the other from the accessory papilla. Both were managed by local resection. In one case endoscopic biopsies and intraoperative frozen sections were negative for carcinoma but histology of the locally resected specimen revealed a focus of invasive adenocarcinoma. Villous adenomas of the duodenum have a high risk of malignant change and foci of carcinoma can be missed on endoscopic biopsy. The literature is reviewed and the clinical, diagnostic, pathological and therapeutic aspects of villous adenomas of the duodenum are discussed.
Subject(s)
Adenocarcinoma/pathology , Adenoma/pathology , Ampulla of Vater , Duodenal Diseases/pathology , Pancreatic Ducts , Adenocarcinoma/complications , Adenocarcinoma/surgery , Adenoma/complications , Adenoma/surgery , Aged , Biopsy , Cholangiopancreatography, Endoscopic Retrograde , Diagnosis, Differential , Duodenal Diseases/complications , Duodenal Diseases/surgery , Endoscopy , Female , Humans , MaleABSTRACT
A patient with ulcerative colitis, sclerosing cholangitis, and mouth ulceration, developed an unusual lesion involving the nasal mucosa. This was thought to represent another extra-intestinal manifestation of inflammatory bowel disease.
