ABSTRACT
Parasites from raw fish can lead to a wide range of clinical manifestations and can be challenging to treat in pregnancy as result of medication exposure of the foetus. We surveyed obstetrician-gynecologists (ob-gyns) in the U.S. to determine their knowledge about the consumption of raw fish during pregnancy. In March 2007, a questionnaire was mailed to members of the American College of Obstetricians and Gynecologists (ACOG) randomly selected to represent all members. Non-responding physicians were sent two additional mailings. Of the 606 ACOG members surveyed, 305 (50%) responded. Most (82%) respondents indicated that eating raw fish is not safe during pregnancy. However, few (19%) knew that thorough freezing kills parasites in fish. Nearly all (94%) respondents thought that parasitic infections can be more challenging to treat in pregnancy. U.S. ob-gyns believe that eating raw fish during pregnancy is not safe; most would benefit from information about how to prevent infection and about treatment.
Subject(s)
Consumer Product Safety , Food Parasitology , Health Knowledge, Attitudes, Practice , Physicians/psychology , Seafood/parasitology , Animals , Female , Food Handling/methods , Health Surveys , Humans , Male , Middle Aged , Obstetrics , Pregnancy , Pregnancy Complications, Parasitic/prevention & control , Risk Factors , Surveys and Questionnaires , United StatesABSTRACT
This study, undertaken as a component of the global Dracunculiasis Eradication Program (DEP), was designed to provide molecular tools to distinguish Dracunculus medinensis, the nematode causing human dracunculiasis, from other tissue-dwelling nematodes, including other Dracunculus species that infect humans and other animals. DNA was extracted from D. medinensis and from a closely related species that infects North American carnivores, D. insignis, so that the genes coding for the small-subunit ribosomal RNA (18S rRNA) of the parasites could be amplified, sequenced and compared. Sequences were obtained for 20 specimens of D. medinensis (from humans in Pakistan, Yemen and six African countries endemic for dracunculiasis) and three of D. insignis (from raccoons trapped in the state of Georgia in the southern U.S.A.). All of the D. medinensis 18S-rRNA sequences were found to be 1819 bases long and identical. The three D. insignis 18S-rRNA sequences were also found to be identical to each other but were 1821 bases long and differed from the D. medinensis 18S- rRNA sequence at eight positions (representing a difference of 0.44%). The 18S-rRNA coding region of a Guinea worm extracted from a dog in Ghana was indistinguishable from that of the D. medinensis isolates from human cases. These results provide the basis for the molecular differentiation of D. medinensis that will permit the DEP to determine, rapidly and accurately, whether a worm recovered from an area considered dracunculiasis-free is a specimen of D. medinensis or not.
Subject(s)
Dracunculiasis/parasitology , Dracunculus Nematode/genetics , Genes, Helminth/genetics , RNA, Ribosomal, 18S/genetics , Animals , Base Sequence , DNA Primers/genetics , Humans , Phylogeny , Polymerase Chain Reaction/methods , RNA, Helminth/genetics , Species SpecificityABSTRACT
Human infection with the sheep nasal botfly Oestrus ovis occurs sporadically. In most cases, there is a history of a strike in the eye by the adult fly. Human O. ovis has been reported rarely from the Americas. We report the first case of O. ovis infection in the Caribbean region, which occurred in an urban area of Barbados. The patient responded to removal of the larvae from the conjunctiva and symptomatic treatment.
Subject(s)
Diptera/growth & development , Eye Infections, Parasitic/diagnosis , Myiasis/diagnosis , Animals , Barbados , Eye Infections, Parasitic/therapy , Female , Goats/parasitology , Humans , Larva , Middle Aged , Sheep/parasitology , Zoonoses/parasitologyABSTRACT
Human infection with the sheep nasal botfly Oestrus ovis occurs sporadically. In most cases, there is a history of a strike in the eye by the adult fly. Human O. ovis has been reported rarely from the Americas. We report the first case of O. ovis infection in the Caribbean region, which occurred in an urban area of Barbados. The patient responded to removal of the larvae from the conjunctiva and symptomatic treatment
Subject(s)
Humans , Animals , Female , Middle Aged , Diptera/growth & development , Eye Infections, Parasitic/diagnosis , Myiasis/diagnosis , Barbados , Goats/parasitology , Eye Infections, Parasitic/therapy , Larva , Sheep/parasitology , Zoonoses/parasitologyABSTRACT
A fatal case of infection with Angiostrongylus cantonensis is reported in a 14-month-old Jamaican boy. Although infection with Angiostrongylus was not considered initially, sections of multiple worms were observed in the brain and lungs at autopsy and confirmed the infection. This is the first reported fatality due to this infection in the Western Hemisphere, and follows shortly after an outbreak of eosinophilic meningitis among a group of travelers to Jamaica. The source of infection in this case could not be determined.
Subject(s)
Angiostrongylus cantonensis/growth & development , Eosinophilia/parasitology , Meningitis/parasitology , Strongylida Infections/parasitology , Animals , Brain/parasitology , Brain/pathology , Eosinophilia/pathology , Fatal Outcome , Female , Humans , Infant , Jamaica , Lung/parasitology , Lung/pathology , Male , Meningitis/pathology , Strongylida Infections/pathologyABSTRACT
Toxoplasma gondii isolates can be grouped into 3 genetic lineages. Type I isolates are considered more virulent in outbred mice and have been isolated predominantly from clinical cases of human toxoplasmosis, whereas types II and III isolates are considered less virulent for mice and are found in humans and food animals. Little is known of genotypes of T. gondii isolates from wild animals. In the present report, genotypes of isolates of T. gondii from wildlife in the United States are described. Sera from wildlife were tested for antibodies to T. gondii with the modified agglutination test, and tissues from animals with titers of 1:25 (seropositive) were bioassayed in mice. Toxoplasma gondii was isolated from the hearts of 21 of 34 seropositive white-tailed deer (Odocoileus virginianus) from Mississippi and from 7 of 29 raccoons (Procyon lotor); 5 of 6 bobcats (Lynx rufus); and the gray fox (Urocyon cinereoargenteus), red fox (Vulpes vulpes), and coyote (Canis latrans) from Georgia. Toxoplasma gondii was also isolated from 7 of 10 seropositive black bears (Ursus americanus) from Pennsylvania by bioassay in cats. All 3 genotypes of T. gondii based on the SAG2 locus were circulating among wildlife.
Subject(s)
Animals, Wild/parasitology , Carnivora/parasitology , Deer/parasitology , Toxoplasma/genetics , Toxoplasmosis, Animal/parasitology , Animals , Antibodies, Protozoan/blood , Biological Assay , Cats , Female , Genotype , Heart/parasitology , Mice , Seroepidemiologic Studies , Toxoplasma/immunology , Toxoplasma/isolation & purification , Toxoplasma/pathogenicity , Toxoplasmosis, Animal/epidemiology , United States/epidemiology , Ursidae/parasitology , VirulenceABSTRACT
Recently, sporadic cases of subconjunctival Onchocerca infection have been reported in dogs in Greece and Hungary. Herein we report further cases from Greece and the results of the molecular analysis of Onchocerca sp. removed from Greek dogs and its Wolbachia endosymbionts. Twenty dogs of various breeds, 1-11 years of age with subconjunctival onchocercosis (4 cases each in right or left eye, 12 cases in both eyes) were presented having similar manifestations. Periorbital swelling, exophthalmos, lacrimation, discharge, photophobia, conjunctival congestion, corneal edema, protrusion of the nictitating membrane, and subconjunctival granuloma or cyst formation were the most important clinical signs. After surgical excision of the periocular masses containing the worms, all animals recovered fully from onchocercosis. Based on the similarities of the clinical picture of the Greek and Hungarian cases, the similar morphology of the Greek and Hungarian isolates, and the identical sequences of the cytochrome oxidase gene of the filarial parasites and that of the 16S ribosomal RNA gene from their Wolbachia endosymbionts, the Onchocerca sp. isolated from dogs in Greece and Hungary appears to belong to the same species.
Subject(s)
DNA, Bacterial/analysis , Dog Diseases/parasitology , Onchocerca/microbiology , Onchocerciasis, Ocular/veterinary , Wolbachia/isolation & purification , Animals , Dog Diseases/microbiology , Dog Diseases/surgery , Dogs , Greece , Onchocerca/isolation & purification , Onchocerciasis, Ocular/microbiology , Onchocerciasis, Ocular/parasitology , Onchocerciasis, Ocular/surgery , Phylogeny , RNA, Ribosomal, 16S/analysis , Symbiosis , Wolbachia/classificationABSTRACT
The results of analysis, by high-performance liquid chromatography coupled with electrochemical detection and by nano-electrospray-ionization, double quadrupole/orthogonal-acceleration, time-of-flight mass spectrometry, indicate that adult Dracunculus medinensis and Schistosoma mansoni both contain the opiate alkaloid morphine and that D. medinesis also contains the active metabolite of morphine, morphine 6-glucuronide. From these and previous observations, it would appear that many helminths are probably using opiate alkaloids as potent immunosuppressive and antinociceptive signal molecules, to down-regulate immunosurveillance responsiveness and pain signalling in their hosts.
Subject(s)
Dracunculus Nematode/chemistry , Morphine/analysis , Schistosoma mansoni/chemistry , Animals , Chromatography, High Pressure Liquid/methods , Morphine Derivatives/analysis , Spectrometry, Mass, Electrospray Ionization/methodsABSTRACT
OBJECTIVE: To study the status of infection with Angiostrongylus cantonensis in wild rats and snails in Jamaica following an outbreak of eosinophilic meningitis amongst tourists. METHODS: One hundred and thirty-one Rattus rattus and 94 R norvegicus were collected, mainly from the eastern portion of Jamaica. The animals were dissected and the heart and pulmonary arteries explored to reveal A cantonensis. Similarly, several species of land snails were collected. A portion of the head/foot was excised, fragmented and placed in separate dishes of digestion fluid (0.01 percent pepsin in 0.7 percent v/v aqeous HCI). Dishes were examined for nematode larvae 4-5 hours and 24 hours post-digestion. A microscopical comparison of larvae with museum specimens confirmed identification. RESULTS: Overall, significantly more R rattus (42.7 percent) than R morvegicus (21.3 percent) were infected (x2=11.28;p<0.0001). Considering only those parishes where 15 or more rats were collected (Hanover, Manchester, St.James, St. Elizabeth, Kingston and St.Andrew, and Portland) the prevalence of rodent infections ranged from 6 to 59 percent. Of three snails and one slug species collected, only Thelidomus asper harboured A cantonensis larvae. This snail was collected from Manchester where the highest prevalence of rodent infections also occurred. CONCLUSIONS: The findings indicate that A cantonensis is present in rats and snails in Jamaica. In the context of reports (1996, 2000) of eosinophilic meningitis on the island, we recommend that public health officials, clinical parasitologists and travel medicine practitioners consider A cantonensis as a causative agent of eosinophilic meningitis in residents and travellers to Jamaica. (AU)
Subject(s)
21003 , Humans , Rats , Angiostrongylus cantonensis/parasitology , Meningitis/epidemiology , Rats/parasitology , JamaicaABSTRACT
OESOPHAGOSTOMUM BIFURCUM: larvae, cultured from human stools collected in northern Ghana, were used to establish experimental infections in monkeys. A patent infection was established in a rhesus monkey (Macaca mulatta) and this infection was used to generate larvae to inoculate additional monkeys. In all, 17 animals were inoculated. Thirteen of 15 animals developed antibodies to the infection between 19 and 62 days post inoculation (PI); two animals had a positive response before inoculation. Four of ten animals developed patent infections between 88 and 134 days and passed eggs in the faeces. Egg shedding was consistent in only one animal, but at low levels of one or two eggs per 2 mg direct smear, and extended over a 400 day period. In the other three animals, egg shedding was sporadic and of only 2-4 weeks duration. In seven animals necropsied between 19 and 22 days PI, one to 17 early fourth-stage larvae were recovered from nodules in the bowel wall; in an eighth animal examined at 314 days, six immature adult worms (early fifth stage) were recovered from nodules in the bowel wall. The morphological features and growth of these recovered larvae are described. Three animals were inoculated with larvae that had been dried for one week at 28 degrees C; two animals began shedding eggs at 128 and 134 days PI, respectively. The present results suggest that the parasite obtained from humans is poorly adapted to lower primate hosts, and supports the concept that Oesophagostomum bifurcum found in humans and monkeys in the same geographical region of northern Ghana and Togo are distinct and that the infections in humans are not likely to represent zoonotic infections acquired from monkeys.
Subject(s)
Monkey Diseases/parasitology , Oesophagostomiasis/parasitology , Oesophagostomum/pathogenicity , Animals , Antibodies, Helminth/biosynthesis , Feces/parasitology , Female , Humans , Intestines/parasitology , Larva/anatomy & histology , Macaca fascicularis , Macaca mulatta , Male , Monkey Diseases/immunology , Oesophagostomiasis/immunology , Oesophagostomum/anatomy & histology , Oesophagostomum/immunology , Species SpecificityABSTRACT
We report a case of Gongylonema infection of the mouth, which caused a migrating, serpiginous tract in a resident of Massachusetts. This foodborne infection, which is acquired through accidental ingestion of an infected insect, such as a beetle or a roach, represents the 11th such case reported in the United States.
Subject(s)
Mouth Diseases/parasitology , Spirurida Infections/parasitology , Spiruroidea , Adult , Albendazole/therapeutic use , Animals , Anthelmintics/therapeutic use , Female , Humans , Massachusetts , Mouth Diseases/drug therapy , Mouth Diseases/surgery , Spirurida Infections/drug therapy , Spirurida Infections/surgery , Spiruroidea/anatomy & histologyABSTRACT
During the summer of 1999, an outbreak of cyclosporiasis occurred among attendees of 2 events held on 24 July in different counties in Missouri. We conducted retrospective cohort studies of the 2 clusters of cases, which comprised 62 case patients. The chicken pasta salad served at one event (relative risk [RR], 4.25; 95% confidence interval [CI], 1.80-10.01) and the tomato basil salad served at the other event (RR, 2.95; 95% CI, 1.72-5.07) were most strongly associated with illness. The most likely vehicle of infection was fresh basil, which was included in both salads and could have been grown either in Mexico or the United States. Leftover chicken pasta salad was found to be positive for Cyclospora DNA by means of polymerase chain reaction analysis, and 1 sporulated Cyclospora oocyst was found by use of microscopy. This is the second documented outbreak of cyclosporiasis in the United States linked to fresh basil and the first US outbreak for which Cyclospora has been detected in an epidemiologically implicated food item.
Subject(s)
Cyclosporiasis/epidemiology , Disease Outbreaks , Ocimum basilicum/parasitology , Adolescent , Adult , Aged , Aged, 80 and over , Animals , Child , Cohort Studies , Cyclospora/genetics , Cyclospora/isolation & purification , Cyclosporiasis/microbiology , Female , Food Parasitology , Humans , Male , Middle Aged , Missouri/epidemiology , Retrospective StudiesABSTRACT
From March 1999 through August 2000, 511 stool samples collected from 11 different primate species in 10 geographically distinct locations in Kenya, East Africa, were screened for the presence of Cyclospora spp. oocysts. Positive samples (43/102, 42%) were identified in vervet monkeys (Cercopithecus aethiops) in 4 of 4 locations; 19/206 (9%) in yellow and olive baboons (Papio cynocephalus, P. anubis, respectively) in 5 of 5 locations; and 19/76 (25%) in black and white colobus monkeys (Colobus angolensis, C. guereza, respectively) from 2 of 3 locations. DNA sequences obtained from 18 S rRNA coding regions from respective subsets of these positive samples were typed as Cyclospora cercopitheci (samples from Cercopithecus aethiops). Cyclospora papionis (samples from Papio cynocephalus and P. anubis), and Cyclospora colobi (samples from Colobus angolensis and C. guereza). Cyclospora oocysts were not detected in samples collected from patas, highland sykes, lowland sykes, blue sykes, DeBrazza, or red-tailed monkeys. A coded map showing the geographic location of the collected samples is given. Stool samples from 1 troop of vervet monkeys were collected over a 12-mo period. Positive samples ranged between 21 and 63%. These results suggest that there is no strongly marked seasonality evident in Cyclospora infection in monkeys as has been noted in human infection. This is further confirmed by the recovery of positive samples collected from vervet monkeys, baboons, and colobus monkeys at all times of the year during this survey. This absence of seasonality in infection is especially notable because of the extreme weather patterns typical of Kenya, where marked rainy and dry seasons occur. A second noteworthy observation is that the striking host specificity of the Cyclospora species initially described was confirmed in this survey. Baboons were only infected with C. papionis, vervet monkeys with C. cercopitheci, and colobus monkeys with C. colobi, despite geographic overlaps of both the monkey and parasite species and wide geographic distribution of each parasite and monkey host.
Subject(s)
Cyclospora/classification , Cyclosporiasis/epidemiology , Primates/parasitology , Animals , Chlorocebus aethiops/parasitology , Colobus/parasitology , Data Collection , Feces/parasitology , Geography , Kenya/epidemiology , Papio/parasitologyABSTRACT
Over the past 10 years, the status of human infection with guinea worm (Dracunculus medinensis) in the Central African Republic (CAR) has been difficult to ascertain. It is unclear if indigenous cases are occurring and whether cases are migrating into the CAR from surrounding countries. A team of investigators visited the CAR in July-August 2000, to attempt to ascertain the presence of indigenous transmission. No cases of true guinea-worm infection (i.e. dracunculiasis) were detected, but three cases of human infection with Onchocerca volvulus, each of which had been misidentified as dracunculiasis, were detected. The unusual presentation of skin blisters and extraction of an intact female O. volvulus are described. As a result of this investigation, and the confusion of onchocerciasis being misidentified as dracunculiasis, the presence of endemic transmission of guinea worm in the CAR remains in question.
Subject(s)
Dracunculiasis/diagnosis , Ectoparasitic Infestations/diagnosis , Onchocerca volvulus , Onchocerciasis/diagnosis , Adult , Animals , Diagnosis, Differential , Humans , MaleABSTRACT
Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.
Subject(s)
Coccidiosis/immunology , Disease Models, Animal , Eucoccidiida/pathogenicity , Animals , Animals, Newborn , Chickens , Disease Susceptibility , Dogs , Ducks , Feces/parasitology , Female , Ferrets , Haplorhini , Humans , Male , Rabbits , Rodentia , SwineABSTRACT
Two dogs, one from California and one from Arizona, were found to have aberrant infections caused by filarial nematodes of the genus Onchocerca. In both cases, the parasites are localized in or near the eye. In one case the worm was located in the cornea and was surgically removed. In the second case, a very marked granulomatous reaction was induced in the retrobulbar space, mimicking an abscess. This eye was enucleated. The worms in both instances were female, and were gravid, i.e. contained microfilariae in utero, indicating that a male worm(s) had been present and mating had occurred. The exact identity of the species of Onchocerca responsible cannot be determined, although the features observed are most like Onchocerca lienalis of cattle. These cases represent the fourth and fifth such cases reported from the US, and are especially interesting because of the unusual location of the worms, the small number of recognized cases, and the similarity to a recent zoonotic human infection.
Subject(s)
Dog Diseases/parasitology , Onchocerca/growth & development , Onchocerciasis, Ocular/veterinary , Animals , Arizona , California , Cornea/parasitology , Dog Diseases/surgery , Dogs , Eye Enucleation/veterinary , Female , Granuloma, Foreign-Body/parasitology , Granuloma, Foreign-Body/veterinary , Male , Onchocerciasis, Ocular/surgeryABSTRACT
Human infections with the intestinal nematode Oesophagostomum bifurcum are commonly found in the Sudan savannah of northern Togo and Ghana. Apparently, the long and hot dry season in this region does not prevent transmission, which is believed to take place through ingestion of the infective, third-stage larvae (L3). Oesophagostomum L3 cultured from human stools, unlike the larvae of Necator americanus, were shown to survive desiccation. In addition, 93% of the O. bifurcum L3 frozen for 24 h at -15 degrees C regained motility when brought back into ambient temperatures. The L3 also survived the acidity of an artificial mixture made to resemble the gastric juices of humans. Desiccated larvae could even be rehydrated in this mixture, indicating the possibility of dust-borne infections. The sturdiness of the L3 is likely to contribute to the high transmission intensity in northern Togo and Ghana.
Subject(s)
Oesophagostomum/growth & development , Animals , Cold Temperature , Desiccation , Feces/parasitology , Gastric Acid , Ghana , Humans , Larva , Macaca fascicularis , Oesophagostomiasis/transmission , TogoABSTRACT
The immunochromatographic (ICT) filariasis test is a rapid screening tool that will be useful for defining the prevalence and distribution of Wuchereria bancrofti as part of the global program to eliminate lymphatic filariasis. To address questions about its usefulness for monitoring control programs, we used the ICT filariasis test to assess residual antigen levels following antifilarial treatment. Our results demonstrate that antigen levels persist in microfilaria-negative persons for up to three years after treatment. Different strategies for monitoring control programs may have to be considered.
Subject(s)
Antigens, Helminth/blood , Filariasis/drug therapy , Filaricides/therapeutic use , Wuchereria bancrofti/immunology , Animals , Chromatography/methods , Diethylcarbamazine/therapeutic use , Filariasis/diagnosis , Humans , Ivermectin/therapeutic use , Wuchereria bancrofti/isolation & purificationABSTRACT
In July 1998, the mother of an 18-month-old boy in rural Tennessee found a triatomine bug in his crib, which she saved because it resembled a bug shown on a television program about insects that prey on mammals. The gut contents of the Triatoma sanguisuga were found, by light microscopy and polymerase chain reaction (PCR), to be infected with Trypanosoma cruzi; PCR products hybridized with T. cruzi-specific oligonucleotide probes. Whole-blood specimens obtained from the child in July and August were negative by buffy-coat examination and hemoculture but positive by PCR and DNA hybridization, suggesting that he had low-level parasitemia. Specimens obtained after treatment with benznidazole were negative. He did not develop anti-T. cruzi antibody; 19 relatives and neighbors also were seronegative. Two of 3 raccoons trapped in the vicinity had positive hemocultures for T. cruzi. The child's case of T. cruzi infection-the fifth reported US autochthonous case-would have been missed without his mother's attentiveness and the availability of sensitive molecular techniques.
Subject(s)
Chagas Disease/diagnosis , Polymerase Chain Reaction , Triatoma/parasitology , Animals , Chagas Disease/transmission , DNA, Protozoan/blood , Female , Humans , Infant, Newborn , Insect Vectors/parasitology , Intestines/parasitology , Male , Parasitemia/diagnosis , TennesseeABSTRACT
We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites included Cyclospora cayetanensis, Giardia intestinalis, Entamoeba coli, Iodamoeba butschlii, Endolimax nana, Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis, and Necator americanus. Preserved stools were examined at various predetermined times (1 week, 1 month, and 3 months) to establish the quality of the initial preservation as well as the suitability of the fixative for long-term storage. At each time point, stool samples in fixatives were examined microscopically as follows: (i) in wet mounts (with bright-field and epifluorescence microscopy), (ii) in modified acid-fast-, trichrome-, and safranin-stained smears, and (iii) with two commercial test kits. At the time points examined, morphologic features remained comparable for samples fixed with 10% formalin and STF. For comparisons of STF- and 10% formalin-fixed samples, specific findings showed that Cyclospora oocysts retained full fluorescence, modified acid-fast- and safranin-stained smears of Cryptosporidium and Cyclospora oocysts were equal in staining quality, and results were comparable in the immunofluorescence assay and enzyme immunoassay commercial kits. Stool fixed in STF and stained with trichrome showed less-than-acceptable staining quality compared with stool fixed in PVA. STF provides an excellent substitute for formalin as a fixative in routine examination of stool samples for parasites. However, modifications to the trichrome staining procedures will be necessary to improve the staining quality for protozoal cysts fixed in STF to a level comparable to that with PVA.