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1.
Avian Dis ; 46(1): 48-52, 2002.
Article in English | MEDLINE | ID: mdl-11922349

ABSTRACT

This study was designed to compare virulence factors of cellulitis-derived Escherichia coli to colisepticemic E. coli in order to clarify whether E. coli associated with cellulitis comprise a unique subset of pathogenic E. coli. Isolates were tested for serotype, capsule, aerobactin production, colicin production, the presence of the iss gene, and serum resistance. Untypable isolates made up the greatest percentage of each group. Serotypes O2 and O78 were the most commonly identified among both groups of isolates. No statistical differences in the distribution of aerobactin or colicin production, capsule, or iss gene were observed between groups. Cluster analysis showed that 90% of the E. coli isolates had greater than 42% livability in serum-resistance tests. No separation of colisepticemic vs. cellulitis E. coli isolates was observed on the basis of SR. Colicin production by E. coli was highly correlated with serum resistance (P = 0.0029). These data suggest that cellulitis E. coli have virulence traits similar to those of colisepticemic E. coli.


Subject(s)
Bacteremia/veterinary , Cellulitis/veterinary , Chickens , Escherichia coli Infections/veterinary , Escherichia coli/pathogenicity , Poultry Diseases/microbiology , Animals , Bacteremia/microbiology , Bacterial Typing Techniques/veterinary , Cellulitis/microbiology , Cluster Analysis , Colicins/biosynthesis , Drug Resistance, Bacterial , Escherichia coli/classification , Escherichia coli/isolation & purification , Escherichia coli Infections/microbiology , Hydroxamic Acids , Virulence
2.
Avian Dis ; 44(1): 23-33, 2000.
Article in English | MEDLINE | ID: mdl-10737641

ABSTRACT

In this study, 294 Escherichia coli isolates from birds with colibacillosis were collected from disease outbreaks throughout the United States and were compared with 75 fecal E. coli isolates of apparently healthy chickens by their possession of several purported virulence genes, resistance to rough-lipopolysaccharide-specific bacteriophages (rLPSr), and elaboration of capsule. Traits were selected for study on the basis of their association with complement resistance. The genes targeted in this study included those encoding colicin V (cvaC) and the outer membrane proteins TraT (traT), OmpA (ompA), and Iss (iss). No significant differences were found between the two groups of isolates in the occurrence of cvaC-, traT-, or ompA-homologous sequences or in rLPSr. Only a few isolates were encapsulated, and the isolates of healthy birds were significantly more likely to be encapsulated than were the isolates of sick birds. However, iss, whether detected through hybridization or amplification, was found in more of the disease-associated isolates than in those of healthy birds. This difference was highly significant. Further, iss sequences were widely distributed among isolates of different serotypes from various avian host species and sites within these hosts. Such results suggest that possession of the iss sequence by an avian E. coli isolate may be a good indicator of that isolate's potential to cause disease. This association warrants further study because iss and the protein it encodes may be useful targets of future colibacillosis control efforts.


Subject(s)
Complement System Proteins/immunology , Escherichia coli Infections/microbiology , Escherichia coli/pathogenicity , Poultry Diseases/microbiology , Animals , Chickens , Disease Susceptibility , Escherichia coli/classification , Escherichia coli/genetics , Escherichia coli Infections/immunology , Gene Amplification , Nucleic Acid Hybridization , Polymerase Chain Reaction/veterinary , Poultry Diseases/immunology , Serotyping , Turkeys
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