Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Angew Chem Int Ed Engl ; 61(1): e202113163, 2022 01 03.
Article in English | MEDLINE | ID: mdl-34734671

ABSTRACT

Mechanosensitive flipper probes are attracting interest as fluorescent reporters of membrane order and tension in biological systems. We introduce PhotoFlippers, which contain a photocleavable linker and an ultralong tether between mechanophore and various targeting motifs. Upon irradiation, the original probe is released and labels the most ordered membrane that is accessible by intermembrane transfer. Spatiotemporal control from photocleavable flippers is essential to access open, dynamic or elusive membrane motifs without chemical or physical interference. For instance, fast release with light is shown to place the original small-molecule probes into the innermost leaflet of the nuclear envelope to image changes in membrane tension, at specific points in time of membrane trafficking along the secretory pathway, or in the inner leaflet of the plasma membrane to explore membrane asymmetry. These results identify PhotoFlippers as useful chemistry tools to enable research in biology.


Subject(s)
Cell Membrane/metabolism , Fluorescent Dyes/metabolism , Nuclear Envelope/metabolism , Cell Membrane/chemistry , Fluorescent Dyes/chemistry , HeLa Cells , Humans , Molecular Structure , Nuclear Envelope/chemistry , Optical Imaging , Photochemical Processes
SELECTION OF CITATIONS
SEARCH DETAIL
...