CBCT data relevant in treatment planning for immediate maxillary molar implant placement.

Background: This study used CBCT images to evaluate the suitability of maxillary first and second molar sites to receive immediate implants. Buccopalatal and mesiodistal widths of maxillary molar inter-radicular septum were evaluated at three different levels (crestal, middle, and apical), in addition to assessments of the root apex and furcation proximities to the sinus floor and comparisons of these measurements between the first and second upper molar sites before extraction. Methods: A total of 427 dental sites from 223 patients were used to measure the buccopalatal and mesiodistal widths of inter-septal/furcal (IRS) bone of maxillary first and second molars and vertical distances from the furcation and from all the root apices to the sinus floor (SF). Results: Mean coronal-most buccopalatal/mesiodistal IRS widths were 7.33/6.52 mm for the first and 6.86/5.85 mm for the second molars (P=0.008). Corresponding mean FSD (furcation-sinus floor) values were 9.69 mm (range: 2.02-24.68 mm) and 8.84 mm (range: 1.48-25.09 mm). Mean distances from all the root apices to SF were <3 mm. The palatal roots of the first molars had higher sinus intrusion rates (28.85%) than their buccal counterparts, while for the second molars, the mesiobuccal roots showed the highest sinus intrusion (37.65%). Conclusion: In the current patient sample, 61.7% of the first and 34% of the second molars had a sufficiently broad IRS to encase a 5-mm-diameter IMI (immediate molar implant) completely. The mean FSD of 9 mm for both molars indicated that some sinus floor elevation would likely be needed unless short implants were used.

Comparison of two digestion strategies on characteristics and differentiation potential of human dental pulp stem cells.

OBJECTIVE: This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DESIGN: DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic features were confirmed by flow cytometry for cell markers STRO-1, cluster of differentiation (CD) 146, CD45, and collagen type-I. The proliferation potential of cells was evaluated by 5-bromo-2'-deoxyuridine (brdU) incorporation assay, and finally they were assessed for multi-lineage differentiation potential. Data were analyzed using one-way analysis of variance and independent t-tests. RESULTS: DPSCs isolated by either method showed similar levels of STRO-1, CD45, and collagen type-I and similar incorporation of brdU (P > 0.05). However, DPSCs obtained by collagenase I/dispase treatment had significantly higher numbers of CD146+ cells and osteogenic and chondrogenic capacities compared to those obtained by treatment with collagenase I alone (P < 0.05). On the other hand, more STRO-1+/CD164-DPSCs were found in the collagenase alone group with higher adipogenic potential. CONCLUSIONS: Different enzyme solutions gave rise to different populations of DPSCs. Dispase enhanced isolation of CD146+ DPSCs probably by disrupting the basement membranes of blood vessels and releasing DPCSs embedded in the perivascular niche. Furthermore, the differentiation potential of DPSCs was influenced by the change in enzyme solution.

Effect of post length and type of luting agent on the dislodging time of metallic prefabricated posts by using ultrasonic vibration.

INTRODUCTION: The purpose of this study was to determine and compare the times needed to dislodge prefabricated titanium posts of different luting lengths with various cements. METHODS: Eighty-one intact extracted, single-canal human teeth were selected and endodontically treated. Specimens were randomly divided into 9 groups, which were arranged according to the post space length (5, 7, and 9 mm) and cement type (zinc phosphate, glass ionomer, and resin cement). Titanium posts were cemented into the post spaces, and after 1 week of storage they were subjected to ultrasonic vibration. The dislodging times were recorded and analyzed by using the Kruskal-Wallis and Mann-Whitney tests (P < .05). RESULTS: A significant difference was observed between the dislodging times on the basis of post length and luting agent (P < .001). There were also significant differences between different cements luted with the same length posts (P < .001). Significant differences were observed between the 5-mm posts and both the 7- and 9-mm posts for each cement, but the differences between the 7-mm and 9-mm posts were not significant (P < .05). CONCLUSIONS: Changes in the cement type and post length alter the time required to dislodge a post with an ultrasonic device.