ABSTRACT
A panel of 27 pig x rodent somatic cell hybrids was produced and characterized cytogenetically. The first step of this study consisted of hybridizing a SINE probe to GTG-banded metaphases of each hybrid clone in order to count and identify the normal pig chromosomes and to detect rearranged ones. The second step consisted of using the DNA of each clone as a probe after pIRS-PCR (porcine interspersed repetitive sequence-polymerase chain reaction) amplification to highly enrich it in pig sequences. These probes, hybridized to normal pig metaphase chromosomes, enabled the identification of the complete porcine complement in the hybrid lines. Whole chromosomes and fragments were characterized quickly and precisely, and results were compared. In addition to this cytogenetic characterization, molecular verification was also carried out by using primers specific to six microsatellites and to one gene previously mapped to pig chromosomes. The results obtained allow us to conclude that we have produced a panel that is informative for all porcine chromosomes. This panel constitutes a highly efficient tool to establish not only assignments of genes and markers but also regional localizations on pig chromosomes.
Subject(s)
Chromosome Mapping , Hybrid Cells , Swine/genetics , Animals , Base Sequence , Cell Line , Cricetinae , DNA Probes , DNA, Complementary , Female , Genetic Markers , In Situ Hybridization, Fluorescence , Male , Metaphase , Molecular Sequence Data , Polymerase Chain ReactionABSTRACT
The status of the sheep map to the end of June 1993 is presented. Mapping information is available for a total of 107 loci comprising 16 anonymous DNA segments. This is an increase of 66 loci since 1990. No loci have been mapped on 10 of the 26 autosomes. Comparison of the cattle (350 loci) and sheep maps confirms their close evolutionary and genetic relationship and will reduce the effort required for their gene mapping.
Subject(s)
Chromosome Mapping , Sheep/genetics , Animals , Biological Evolution , Cattle/genetics , Genotype , Terminology as TopicABSTRACT
A review of the present status of the porcine gene map is given with references. A total of 84 loci have now been studied, and genes have been assigned to 17 chromosomes. Among them, six chromosomes are defined by only one marker. No loci have been attributed yet to three chromosomes.
Subject(s)
Chromosome Mapping , Swine/genetics , Animals , Genetic Linkage , Genetic MarkersABSTRACT
A high-resolution GTG-banded karyotype of the pig was obtained after methotrexate-induced cell synchronization. A diagrammatic representation of the banding pattern at the 539-band level is presented.
Subject(s)
Chromosome Banding , Swine/genetics , Animals , Cells, Cultured , Karyotyping , MaleSubject(s)
Chromosome Mapping , Animals , Genetic Linkage , Genetic Markers , Humans , Species SpecificityABSTRACT
A cell synchronization technique allowed the high-resolution GTG-banding pattern of rabbit chromosomes to be determined. A karyotype of the late-prophase 622-band stage is presented.
Subject(s)
Chromosome Banding/methods , Animals , Fibroblasts/ultrastructure , Karyotyping , RabbitsABSTRACT
Using as a probe pig genomic DNA, including the complete interferon alpha gene, we have mapped the leukocyte interferon gene on pig chromosome 1 by in situ hybridization. A total of 196 silver grains were noted on the 106 metaphases scored: 31% of the grains were observed on chromosome 1, and 67% of these were localized in the region 1q2.2----q2.7.
Subject(s)
Chromosome Mapping , Interferon Type I/genetics , Swine/genetics , Animals , Karyotyping , Nucleic Acid HybridizationABSTRACT
The pig major histocompatibility complex (SLA) was mapped by in situ hybridization. The probe used was a human cDNA containing most of the coding sequence for HLA-B7. The SLA complex was localized to the region p1.4----q1.2 on chromosome 7.
Subject(s)
Major Histocompatibility Complex , Swine/genetics , Animals , Chromosome Mapping , DNA , Genetic Markers , HLA Antigens/genetics , HLA-B7 Antigen , Humans , Nucleic Acid Hybridization , Species SpecificityABSTRACT
The syntenic alpha and beta casein genes were localized in the rabbit by chromosomal in situ hybridization, using a mixture of two radioactive cDNA probes corresponding to these two genes. Highly significant labeling was observed on chromosome 12. A total of 175 silver grains was found on chromosomes in the 193 mitoses studied; 18% of the grains were on chromosome 12, and 42% of the grains on this chromosome were localized to the 12q24 region. Statistical analysis revealed that this labeling was highly significant.
