ABSTRACT
Abdominal fat tissue aspiration was used in 22 long-term hemodialysis patients (5-17 years). Fourteen of these patients had carpal tunnel syndrome and amyloid deposits of beta 2-microglobulin in the synovium. One patient had a spontaneous rupture of the spleen with amyloid deposits in spleen vessels. Seven other patients presented carpal tunnel syndrome and/or articular pains, and radiological lytic lesions in bone, strongly suggesting an amyloid origin. As a control group, in 22 patients with biopsy-proven amyloidosis, abdominal fat tissue aspirates were performed and were studied under the same conditions: by light microscopy these tissues were stained with Congo red and examined with a polarizing microscope; these specimens were also studied by electron microscopy. In all hemodialyzed patients, no amyloid deposit was present in fat tissue with Congo red staining and by electron microscopy. On the contrary, amyloid was observed in 17 of 22 cases in other types of amyloidosis. It seems that this method which has been proved to be simple and sensitive for the diagnosis of systemic amyloidosis is not a good marker for the presence of amyloid in long-term hemodialysis patients.
Subject(s)
Adipose Tissue/pathology , Amyloidosis/diagnosis , Renal Dialysis/adverse effects , Abdominal Muscles/pathology , Adult , Aged , Amyloidosis/etiology , Biopsy, Needle , Female , Humans , Male , Microscopy, Electron , Middle Aged , Staining and Labeling , Time FactorsABSTRACT
Separate kallikrein excretions [RIA of generated Bradykinins (BK)] and renal functions were measured in 32 two-kidney, one-clip Goldblatt hypertensive (GH) and in 16 sham-operated (SO) rats either one, two, three or four weeks after operation. Mean blood pressure and plasma renin activity were higher in GH rats than in the respective controls. In GH rats, kallikrein excretion was lower from the clipped kidney than from the controlateral one (114 +/- 63 vs 220 +/- 87 ng BK min-1 per 30 min urine collection at week 1, P less than 0.05; 151 +/- 94 vs 425 +/- 125 ng BK min-1 per 30 min urine volume at week 4, P less than 0.01). While the excretion was normal from the controlateral kidney, GH rats had lower total kallikrein excretions than SO rats (335 +/- 138 vs 476 +/- 81 ng BK min-1 per 30 min urine volume at week 1, P less than 0.05; 556 +/- 179 vs 1078 +/- 191 ng BK min-1 per 30 min urine volume at week 4, P less than 0.01). Urinary kallikrein excretion is decreased in 2K-1C Goldblatt hypertension from one week to four weeks after clipping on account of an exclusive reduction in that from the stenotic kidney.
