1.
J Virol Methods
; 31(2-3): 211-7, 1991.
Article
in English
| MEDLINE
| ID: mdl-1864906
ABSTRACT
A method was developed for concentrating infectious pancreatic necrosis virus from hatchery water using positively charged 1-MDS filters. The method consists of passing large volumes (Ca. 1001) of hatchery water through 1-MDS microporous filter followed by the elution of the adsorbed virus using a high pH buffer. The virus adsorbed efficiently to 1-MDS filters when the pH of the water was 5.5 and was eluted optimally with 3% beef extract solution (pH 10). This procedure permitted the processing of 100 1 of hatchery water which resulted in a 300-fold reduction in the volume of water and greater than 90% recovery of the seeded virus.
Subject(s)
Aluminum Compounds , Reoviridae/isolation & purification , Trout/microbiology , Water Microbiology , Adsorption , Aluminum , Aluminum Chloride , Animals , Cells, Cultured , Chlorides , Culture Media , Hydrogen-Ion Concentration
2.
Proc Soc Exp Biol Med
; 167(4): 480-4, 1981 Sep.
Article
in English
| MEDLINE
| ID: mdl-7279926