ABSTRACT
The clinical value of current and future nanomedicines can be improved by introducing patient selection strategies based on noninvasive sensitive whole-body imaging techniques such as positron emission tomography (PET). Thus, a broad method to radiolabel and track preformed nanomedicines such as liposomal drugs with PET radionuclides will have a wide impact in nanomedicine. Here, we introduce a simple and efficient PET radiolabeling method that exploits the metal-chelating properties of certain drugs (e.g., bisphosphonates such as alendronate and anthracyclines such as doxorubicin) and widely used ionophores to achieve excellent radiolabeling yields, purities, and stabilities with 89Zr, 52Mn, and 64Cu, and without the requirement of modification of the nanomedicine components. In a model of metastatic breast cancer, we demonstrate that this technique allows quantification of the biodistribution of a radiolabeled stealth liposomal nanomedicine containing alendronate that shows high uptake in primary tumors and metastatic organs. The versatility, efficiency, simplicity, and GMP compatibility of this method may enable submicrodosing imaging studies of liposomal nanomedicines containing chelating drugs in humans and may have clinical impact by facilitating the introduction of image-guided therapeutic strategies in current and future nanomedicine clinical studies.
Subject(s)
Copper Radioisotopes , Liposomes , Nanomedicine , Positron-Emission Tomography , Breast Neoplasms/diagnostic imaging , Female , Humans , Manganese , Radioisotopes , Tissue Distribution , ZirconiumABSTRACT
PURPOSE OF REVIEW: Vision rehabilitation has been considered as an adjunct support option or just the dispensing of magnifiers in the management of macular degeneration. RECENT FINDINGS: New research suggests that vision rehabilitation can cause physical changes in the brain that help patients adjust to permanent vision loss. SUMMARY: Vision rehabilitation is related to physical changes that occur in the brain. Its use in conjunction with clinical low-vision programs may pave the way for developing better rehabilitation techniques for patients with macular blindness.
