ABSTRACT
We studied osteogenesis induction after implantation of porous cryogenically structured 3D scaffolds based on chitosan, alginate, and serum albumin and containing a bioregulator from bovine serum into in vivo modeled experimental bone defect in rats. It was shown that such 3D-matrices are effective as osteoconductors due to their effect on osteoblast precursors. All types of bioregulator-loaded 3D materials promote active bone repair, which manifested in restoration of the dense bone tissue, formation of the bone marrow, and the recovery of osteons on day 14 after surgery. In animals receiving implantation of control 3D scaffolds without the bioregulator, the formation of dense fibrous tissue and spongy bone was observed by this term.
Subject(s)
Osteogenesis/physiology , Tissue Scaffolds/chemistry , Animals , Bone Regeneration/physiology , Male , Mesenchymal Stem Cells/cytology , Osteoblasts/cytology , Porosity , Rats , Rats, Wistar , Tissue EngineeringABSTRACT
15 patients with CIDP (8 men, 7 women) at the age of 39-65 have been observed. The duration of the disease varied from 1 to 9 years. The authors have used in diagnostics the WHO's criteria as well as standards of needle and stimulative electro-neuromyography (ENMG). CIDP was shown in the study to occur more frequently after 50 with male prevalence. ENMG revealed 8 patients with compromised myelin and 7 patients with combined myelin and axon abnormalities, 3 patients with nerve impulse block conduction. The following variants of CIDP were distinguished: motor-sensor--7; motor-autonomic--3; pure motor--2; multifocal mononeuropathy with conduction blocks.
