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1.
Article in Russian | MEDLINE | ID: mdl-6778034

ABSTRACT

The data are presented on comparative evaluation of the growth inhibition test (GIT), the diffuse precipitation test in agar gel (DPTAG) and the complement fixation test (CFT) in the identification of Mycoplasma isolated from monkeys. The results obtained in the study of 4 Mycoplasma strains from the internal organs of monkeys in the CFT and the DPTAG with hyperimmune rabbit sera provided by Microbiological Associates, Bethesda, Maryland, USA, allowed these Mycoplasma to be identified as M. laidlawii (l 6916, p 6240 and M 6802) and M. hominis (C 7034), which was confirmed by the study of the biological properties of these strains and in the GIT. The CFT and the DP-TAG, equally to the GIt and other serological tests, can be used for identification of newly isolated Mycoplasma strains.


Subject(s)
Acholeplasma laidlawii/isolation & purification , Haplorhini/microbiology , Mycoplasma/isolation & purification , Animals , Complement Fixation Tests , Immunodiffusion , Kidney/microbiology , Lung/microbiology , Mycoplasma pneumoniae/isolation & purification , Species Specificity , Spleen/microbiology , Urine/microbiology
2.
Article in Russian | MEDLINE | ID: mdl-452781

ABSTRACT

A complex of immunological cell tests with M. pneumoniae antigen (the lymphocyte blast-cell transformation test, the allergic neutrophil alteration test) was carried out in order to establish the correlation between the results of positive seroconversion and the sepcific immunological reactivity of lymphoid cells in pneumonia patients. Mycoplasmic cutireactive allergen, when used for the accelerated diagnosis of mycoplasmic pneumonia in humans, was shown to be specific and safe. Cuti-allergic tests with mycoplasmic allergen allowed to diagnose mycoplasmic pneumonia at early stages (beginning from days 5--7), which ensures the possibility of indicating etiotropic treatment to patients in due time.


Subject(s)
Mycoplasma Infections/diagnosis , Pneumonia/diagnosis , Allergens , Cell Migration Inhibition , Complement Fixation Tests , Humans , Hypersensitivity, Delayed/diagnosis , In Vitro Techniques , Lymphocyte Activation , Mycoplasma/immunology , Neutrophils/immunology , Serologic Tests/methods , Skin Tests , Time Factors
5.
Biull Eksp Biol Med ; 87(1): 30-2, 1979 Jan.
Article in Russian | MEDLINE | ID: mdl-760841

ABSTRACT

The light beating spectroscopy technique was used for recording the results of serological reactions without visual phenomena. The investigated systems consisted of the antigens of influenza virus or Mycoplasma pneumoniae and the corresponding sera. A principal possibility of detecting the antigen-antibody complexes in serological reactions without visual phenomena by the light beating spectroscopy technique was demonstrated.


Subject(s)
Antigen-Antibody Complex/analysis , Antigens, Bacterial , Antigens, Viral , Coronaviridae/immunology , Immune Sera , Influenza A virus/immunology , Mycoplasma/immunology
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