[The detection and identification of alpha-pyrrolidino-valerophenone (α-PVP) and its metabolites in the objects of the forensic chemical examination]. / Obnaruzhenie α-pirrolidinovalerofenona (α-PVP) i ego metabolitov v ob''ektakh sudebno-khimicheskogo issledovaniia.

The combined application of the IK 200609 chemical toxicological analyzer and the diagnostic biosensor (reagent) for the determination of synthetic cations (with due regard for compliance with the instruction for analysis) made it possible to detect during 15 minutes the presence of cations in the urine samples at the concentration of 376.64 ng/ml. This result was further confirmed by HPLC-Ms/MS and GH-MS. The use of the analyzer allowed the screening of the urine samples to be performed within several minutes at the preliminary stage of the study. A simplified method of tissue sample preparation with the use of the kits for extraction and solid-phase purification is proposed together with a variant of blood sample preparation with the use of filtration. The proposed approach can be employed for the rapid detection and identification of alpha-pyrrolidino-valerophenone (α-PVP) and its metabolites in urine, blood and tissues of various organs for the purpose of practical toxicological investigations in the framework of forensic chemical expertise.

[Current approaches to analysis of narcotic drugs in biological substances].

A method of analysis of basic narcotic drugs in urine is described. It consists in isolation by solid phase extraction, derivation and identification by gas chromatography with mass-selective detector. The approaches applied in the above method can be used for analysis of narcotic drugs undetectable by preliminary investigations as well as for urine examination for pharmacological or other biologically active substances.