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1.
Orthopedics ; 44(6): e753-e756, 2021.
Article in English | MEDLINE | ID: mdl-34618630

ABSTRACT

Surgical helmet systems (SHSs) have been used to decrease iatrogenic contamination to prevent periprosthetic joint infections. However, the use of SHSs has been controversial. Therefore, the purpose of this study was to investigate iatrogenic contamination of traditional surgical attire (TSA), SHSs, and SHSs with delayed ventilation (SHS-DV) (helmet fan not turned on until surgeon gowned and gloved). A total of 180 orthopedic surgical procedures were prospectively enrolled and randomized into one of three cohorts. The TSA cohort included any orthopedic procedures, while the SHS and SHS-DV cohorts included arthroplasty procedures. Cultures were obtained from bilateral forearms, axillae, the sternum, and face shields for SHS groups. There were 60 surgeries in each group. The rate of positive cultures was calculated for each cohort and stratified by location and type of microorganism. The positive culture rates were 15% in the TSA, 25% in the SHS, 18% in the SHS-DV cohorts. The positive swab culture rates were 6% in the TSA, 7% in the SHS, and 4% in the SHS-DV cohorts. The positive culture rate was highest from the forearms in the TSA cohort (10%), the face shield in the SHS cohort (20%), and the chest in the SHS-DV cohort (7%). Coagulase-negative Staphylococcus was the most common bacteria cultured. The overall bacterial contamination rates were similar between the TSA and the SHS cohorts, with a lower rate in the SHS-DV cohort. Waiting to initiate airflow in SHSs and treating the shields as contaminated may reduce iatrogenic contamination. [Orthopedics. 2021;44(6):e753-e756.].


Subject(s)
Orthopedic Procedures , Orthopedics , Surgical Attire , Head Protective Devices , Humans , Iatrogenic Disease/prevention & control , Orthopedic Procedures/adverse effects
2.
J Hand Surg Asian Pac Vol ; 22(3): 350-354, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28774254

ABSTRACT

BACKGROUND: The purpose of this study is to determine whether a Stener lesion can be created while testing stability of the ulnar collateral ligament (UCL) of the thumb. Testing was performed in a manner that reproduced clinical examination. METHODS: Six fresh frozen hand and forearm specimens underwent sequential sectioning of the accessory UCL, the proper UCL, and the ulnar sagittal band. Measurements of radial deviation of the metacarpophalangeal (MCP) joint were taken with the thumb in neutral rotation, pronation and supination, both with 0 degrees and with 30 degrees of flexion of the MCP joint. Visual examination was performed to assess the presence of a Stener lesion. RESULTS: No Stener lesion was created in any position as long as the fascial origin of the ulnar sagittal band at the adductor pollicis longus remained intact. After creating a defect in the ulnar sagittal band, a Stener lesion was created in two specimens, but only when the thumb was flexed and supinated. Pronation provided more stability, and supination provided less stability, with one or both components cut, especially when testing at 30° of flexion. Compared to both components cut without flexion or rotation, there was a statistically significant difference in angulation with the 30 degrees of MCP joint flexion in both neutral rotation in supination. CONCLUSIONS: Performing a physical examination to assess the amount of instability of an ulnar collateral ligament injury did not create a Stener lesion if the exam was performed in a controlled, gentle manner with the thumb held without rotation. If the thumb is held in neutral rotation during the exam, an iatrogenic Stener lesion should not be created.


Subject(s)
Collateral Ligaments/injuries , Metacarpophalangeal Joint/injuries , Physical Examination/adverse effects , Range of Motion, Articular/physiology , Thumb/injuries , Aged, 80 and over , Cadaver , Female , Forearm , Humans , Male , Pronation/physiology , Supination/physiology
3.
Clin Cancer Res ; 17(19): 6140-50, 2011 Oct 01.
Article in English | MEDLINE | ID: mdl-21825040

ABSTRACT

PURPOSE: To evaluate the nature of cyclin-dependent kinase 5 (CDK5) hyperactivity in pancreatic cancer progression. EXPERIMENTAL DESIGN: We used genetic, biochemical, and molecular biology methods to investigate the nature and function of overexpression of CDK5 and its activators p35 and p39 during the progression of pancreatic cancer. RESULTS: Amplification of the CDK5 gene or either of its main activators, p35 and p39, was observed in 67% of human pancreatic ductal adenocarcinoma (PDAC). CDK5, p35, and p39 were rarely expressed in pancreatic ducts whereas more than 90% of PDACs had increased levels of CDK5 and p35. Increased levels of CDK5, p35, and p39 protein were observed in several pancreatic cancer cell lines. Inhibition of CDK5 kinase activity using a CDK5 dominant-negative mutant or the drug roscovitine significantly decreased the migration and invasion of pancreatic cancer cells in vitro. Increased CDK5 kinase activity was also observed in immortalized human pancreatic nestin-expressing (HPNE) cells expressing a mutant form of K-Ras (G12D) compared with HPNE cells expressing native K-Ras. G12D K-Ras increased cleavage of p35 to p25, a stable and greater activator of CDK5, thus implicating a role for CDK5 in early progression of PDAC. Inhibition of the signaling cascade downstream of mutant K-Ras (G12D) that involves mitogen-activated protein/extracellular signal-regulated kinase, phosphoinositide 3-kinase, or CDK5 decreased p25 protein levels. CONCLUSION: These results suggest that mutant K-Ras acts in concert with CDK5 and its activators to increase malignant progression, migration, and invasion of pancreatic cancer cells.


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Carcinoma, Pancreatic Ductal/enzymology , Cell Cycle Proteins/metabolism , Cyclin-Dependent Kinase 5/metabolism , Genes, ras , Pancreatic Neoplasms/metabolism , Adenocarcinoma , Carcinoma, Pancreatic Ductal/pathology , Cell Line, Tumor , Cyclin-Dependent Kinase 5/antagonists & inhibitors , Cyclin-Dependent Kinase 5/genetics , Disease Progression , Enzyme Activation/genetics , Gene Amplification , Humans , Mutation , Neoplasm Invasiveness , Nerve Tissue Proteins/metabolism , Pancreatic Neoplasms/genetics , Purines/pharmacology , Roscovitine
4.
Clin Cancer Res ; 14(19): 5995-6004, 2008 Oct 01.
Article in English | MEDLINE | ID: mdl-18829478

ABSTRACT

PURPOSE: We investigated the contribution of Sonic hedgehog (SHH) to pancreatic cancer progression. EXPERIMENTAL DESIGN: We expressed SHH in a transformed primary ductal-derived epithelial cell line from the human pancreas, transformed hTert-HPNE (T-HPNE), and evaluated the effects on tumor growth. We also directly inhibited the activity of SHH in vivo by administering a blocking antibody to mice challenged orthotopically with the Capan-2 pancreatic cancer cell line, which is known to express SHH and form moderately differentiated tumors in nude mice. RESULTS: Our data provide evidence that expression of SHH influences tumor growth by contributing to the formation of desmoplasia in pancreatic cancer. We further show that SHH affects the differentiation and motility of human pancreatic stellate cells and fibroblasts. CONCLUSIONS: These data suggest that SHH contributes to the formation of desmoplasia in pancreatic cancer, an important component of the tumor microenvironment.


Subject(s)
Fibrosis/pathology , Gene Expression Regulation, Neoplastic , Hedgehog Proteins/physiology , Pancreatic Neoplasms/metabolism , Pancreatic Neoplasms/therapy , Animals , Cell Line, Transformed , Cell Line, Tumor , Cell Transformation, Neoplastic , Fibroblasts/metabolism , Fibrosis/metabolism , Hedgehog Proteins/metabolism , Humans , Mice , Mice, Nude , Neoplasm Invasiveness , Neoplasm Transplantation , Pancreas/metabolism
5.
J Biol Chem ; 283(40): 26985-95, 2008 Oct 03.
Article in English | MEDLINE | ID: mdl-18625714

ABSTRACT

MUC1, a transmembrane mucin, is a key modulator of several signaling pathways that affect oncogenesis, motility, and cell morphology. The interaction of MUC1 cytoplasmic tail (MUC1CT) with signal transducers and its nuclear translocation and subsequent biological responses are believed to be regulated by phosphorylation status, but the precise mechanisms by which this occurs remain poorly defined. We detected a novel association between the Met receptor tyrosine kinase and the MUC1CT. Met catalyzed phosphorylation of tyrosine at YHPM in the MUC1CT. Stimulation of S2-013.MUC1F pancreatic cancer cells with hepatocyte growth factor facilitated nuclear localization of MUC1CT, as determined by real time confocal imaging analysis. MUC1 overexpression also facilitated faster turnover of Met. Phosphorylation of MUC1CT by Met enhanced its interaction with p53, which led to suppression of AP1 transcription factor activity through interactions at the MMP1 promoter, ultimately leading to reduced transcription of MMP1. This correlated with a decrease in hepatocyte growth factor-induced invasiveness when MUC1 was overexpressed. The results demonstrate that MUC1 modulates Met-mediated oncogenic signaling in cancer.


Subject(s)
Cell Nucleus/metabolism , Gene Expression Regulation, Enzymologic/drug effects , Hepatocyte Growth Factor/pharmacology , Matrix Metalloproteinase 1/biosynthesis , Mucin-1/metabolism , Proto-Oncogene Proteins/metabolism , Receptors, Growth Factor/metabolism , Tumor Suppressor Protein p53/metabolism , Active Transport, Cell Nucleus/drug effects , Active Transport, Cell Nucleus/physiology , Cell Line, Tumor , Gene Expression Regulation, Enzymologic/physiology , Hepatocyte Growth Factor/metabolism , Humans , Promoter Regions, Genetic/physiology , Protein Structure, Tertiary/physiology , Proto-Oncogene Proteins c-met , Signal Transduction/drug effects , Transcription Factor AP-1/metabolism
6.
Cancer Res ; 67(21): 10222-9, 2007 Nov 01.
Article in English | MEDLINE | ID: mdl-17974963

ABSTRACT

Perineural invasion in pancreatic adenocarcinoma, a common pathologic phenomenon whereby cancer cells invade and intimately contact the endoneurium of pancreatic nerves, is thought to contribute to both pain and local disease recurrence. MUC1, a type I transmembrane mucin that can affect the adhesive properties of cells, contains a large extracellular tandem repeat domain, which is heavily glycosylated in normal epithelia, but is overexpressed and differentially glycosylated in pancreatic cancer. This altered glycosylation includes the shortened core I O-glycans for monosialyl and disialyl T antigens. Myelin-associated glycoprotein (MAG), a membrane-bound protein expressed on oligodendrocytes and Schwann cells, binds myelin to neurons. MAG's preferred ligands are derivatives of the monosialyl and disialyl T antigen. We investigated whether MUC1 is a counter-receptor for MAG and if their interaction contributed to pancreatic perineural invasion. Results showed that MAG binds pancreatic cells expressing MUC1, that this binding is sialidase-sensitive, and that MAG physically associates with MUC1. Heterotypic adhesion assays between pancreatic cancer cells and Schwann cells revealed that increased expression of MUC1 or MAG enhanced adhesion. Conversely, specific inhibition of MAG or sialyl-T MUC1 partially blocked adhesion. Immunohistochemical analysis of pancreatic perineural invasion showed the expression of both MUC1 and MAG. These results support the hypothesis that the adhesive interactions between MUC1 and MAG are of biological significance in pancreatic cancer perineural invasion.


Subject(s)
Cell Adhesion , Lectins/physiology , Mucin-1/physiology , Myelin-Associated Glycoprotein/physiology , Pancreatic Neoplasms/pathology , Perineum/pathology , Animals , CHO Cells , Cell Line, Tumor , Cricetinae , Cricetulus , Humans , Immunoprecipitation , Neoplasm Invasiveness , Schwann Cells/pathology , Sialic Acid Binding Immunoglobulin-like Lectins , Signal Transduction
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