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1.
Vopr Virusol ; 54(2): 44-7, 2009.
Article in Russian | MEDLINE | ID: mdl-19459412

ABSTRACT

The new highly sensitive test system "DS-EIA-HBsAg-0.01" (Priority Certificate No. 2006129019 of August 10, 2006) in detecting hepatitis B surface antigen (HBsAg) was assessed. The sensitivity of the test was estimated using the federal standards sample HBsAg 42-28-311-06, panels' samples Boston Biomedica Inc. (West Bridgewater, Mass, USA) and ZeptoMetrix Corp. (Buffalo, NY, USA). The findings have indicated that "DS-EIA-HBsAg-0.01" is equally effective in detecting different subtypes of HBsAg during a seroconversion period earlier than alternative assays. Along with its high analytical and diagnostic sensitivity, the system shows a high diagnostic specificity.


Subject(s)
Hepatitis B Surface Antigens/analysis , Hepatitis B/diagnosis , Reagent Kits, Diagnostic , Hepatitis B/immunology , Humans , Sensitivity and Specificity
2.
Phys Rev Lett ; 100(13): 133007, 2008 Apr 04.
Article in English | MEDLINE | ID: mdl-18517948

ABSTRACT

Exact and general analytic expressions are reported for the integrated intensity and the width of collision-induced absorption (CIA) and collision-induced scattering (CIS) bands by gases of centrosymmetric linear molecules. These expressions provide significant insight and allow assignment of partial second moments to the degrees of freedom of the colliding molecules. The expressions are applied to ambient CO(2), whose collisional spectra are reputed to be useful probes for terrestrial and planetary atmospheres. Compelling evidence of the substantial role of hitherto missing polarization and polarizability mechanisms is provided and is in remarkable agreement with experimental observation. Our findings allow the long-overdue simple interpretation of CIA and CIS by CO(2)-CO(2) without the need to resort to short-range interactions to offset the discrepancies between theory and experiment.

3.
Burns ; 30(6): 552-6, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15302420

ABSTRACT

The level of the total and dimeric (oligomeric) forms of soluble CD38 antigen (sCD38) has been determined by an ELISA sandwich method in serum from burned patients (n=18) and healthy volunteers (n = 25). The serum level of total sCD38 was insignificantly increased in patients at the stage of burn shock (135 +/- 10.8 U/ml, mean +/- S.E.M.) and significantly decreased between 4 and 14 postburn days in comparison with volunteers (69.5 +/- 10.8 U/ml versus 121 +/- 7.8 U/ml, P < 0.05). The serum level of soluble dimeric CD38 in burned patients was statistically lower than normal during all periods of observation (45.3 +/- 8.8 and 130 +/- 6.2 U/ml, respectively, P < 0.01). The relative number of CD38(+) lymphocytes was increased during the period of shock in comparison with healthy volunteers (21 +/- 1.6% versus 13 +/- 1.1%, P < 0.05). There were no correlations between number CD38(+) lymphocytes and total sCD38 or dimeric sCD38 serum levels. These data suggest that the mCD38 expression and serum level of total sCD38 are a markers the early postburn lymphocytes activation. The decrease of dimeric sCD38 level can reflect its dissociation to monomeric form in burned patients.


Subject(s)
ADP-ribosyl Cyclase/blood , Antigens, CD/blood , Burns/blood , ADP-ribosyl Cyclase 1 , Adolescent , Adult , Burns/immunology , Enzyme-Linked Immunosorbent Assay/methods , Humans , Lymphocyte Activation/immunology , Lymphocytes/immunology , Membrane Glycoproteins , Solubility , Time Factors
5.
Vopr Pitan ; (4): 40-2, 1994.
Article in Russian | MEDLINE | ID: mdl-7817530

ABSTRACT

Dietary effects of polyunsaturated fatty acids (PUFA) omega-3 on lipid peroxidation (LPO) and antioxidant system were studied in 73 patients with ischemic heart disease, hyperlipoproteinemia (HLPE) type IIa, IIb, IV and essential hypertension. Eiconol-enriched antiatherosclerotic diet has more potent hypolipidemic, hypotensive and thrombolytic action in association with inhibition of LPO, enhances SOD activity, keeps red cell catalase within normal. Vitamin E concentrations were not changed. It is suggested that eiconol addition to antiatherosclerotic diet causes no LPO induction and is pathognomonic for HLPE, hypertension and IHD patients.


Subject(s)
Antioxidants , Coronary Disease/diet therapy , Dietary Fats, Unsaturated/administration & dosage , Fatty Acids, Omega-3/administration & dosage , Hyperlipoproteinemias/diet therapy , Hypertension/diet therapy , Lipid Peroxidation , Adult , Aged , Catalase/blood , Erythrocytes/enzymology , Evaluation Studies as Topic , Female , Humans , Male , Middle Aged , Vitamin E/blood
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