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1.
Physiol Int ; 110(2): 150-159, 2023 Jun 12.
Article in English | MEDLINE | ID: mdl-37130019

ABSTRACT

Physical exercise represents one of the most effective approaches to anti-aging. The goal of this study was to verify the effects of different modes and intensities of exercise on longevity proteins in the skeletal muscle in midlife. Middle-aged mice were trained in aerobic or resistance exercise for 8 weeks, and the changes in sirtuin 1 (SIRT1), adenosine monophosphate-activated kinase (AMPK), and mammalian target of rapamycin (mTOR) pathways in the skeletal muscle were evaluated by western blotting. Long-term exercise had no effects on skeletal muscle SIRT1 abundance, whereas high-intensity aerobic exercise increased AMPK phosphorylation and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). Low-intensity resistance exercise facilitated Akt/mTOR/p70 ribosomal protein kinase S6 (p70S6K) signaling but did not induce muscle hypertrophy. Conversely, high-intensity resistance exercise stimulated muscle hypertrophy without phosphorylation of mTOR signaling-related proteins. These results suggest the importance of setting exercise modes and intensities for anti-aging in midlife.


Subject(s)
Longevity , Transcription Factors , Mice , Animals , Sirtuin 1/metabolism , AMP-Activated Protein Kinases/metabolism , TOR Serine-Threonine Kinases/metabolism , Muscle, Skeletal/physiology , Hypertrophy/metabolism , Mammals/metabolism
2.
In Vitro Cell Dev Biol Anim ; 42(1-2): 1-3, 2006.
Article in English | MEDLINE | ID: mdl-16618204

ABSTRACT

A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35 degrees C, and the maximum growth rate was at 25 degrees C. The chromosome number ranged from 70-239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.


Subject(s)
Cell Line , Fat Body/cytology , Moths , Animals , Cell Proliferation , Cell Shape , Culture Media , Ecdysterone/metabolism
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