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J Immunother ; 24(6): 493-501, 2001.
Article in English | MEDLINE | ID: mdl-11759072

ABSTRACT

Tumor lysate-pulsed dendritic cells were used to generate nodal effector T cells in the murine MCA 205 tumor model. Dendritic cells were derived from bone marrow and cultured in granulocyte-macrophage colony-stimulating factor/interleukin 4 before pulsation with tumor lysate. Multiple subcutaneous administrations of tumor lysate-pulsed dendritic cells (TP-DCs) resulted in an approximately eightfold hypertrophy of the vaccine draining nodes, with an increased influx of dendritic (CD11c+/CD80+) cells and B (B220+) cells. The vaccine-primed lymph node (VPLN) cells were secondarily activated with anti-CD3/interleukin 2 and exhibited specific interferon-gamma release to tumor antigen. The adoptive transfer of TP-DC VPLN cells resulted in regression of established 3-day pulmonary metastases. The antitumor reactivity of TP-DC VPLN cells was comparable to anti-CD3/interleukin 2 activated tumor-draining lymph node cells. However, the admixture of keyhole limpet hemocyanin (KLH) with tumor lysate during pulsation of dendritic cells significantly enhanced the induction of tumor-reactive VPLN cells. Tumor lysate-pulsed dendritic cells can be used as a strategy to generate effector T cells for adoptive immunotherapy.


Subject(s)
Cancer Vaccines/immunology , Dendritic Cells/immunology , Fibrosarcoma/therapy , Adjuvants, Immunologic , Adoptive Transfer , Animals , Antigens, Neoplasm , Disease Models, Animal , Female , Fibrosarcoma/immunology , Haptens/pharmacology , Hemocyanins/pharmacology , Immunophenotyping , Immunotherapy , Interferon-gamma/metabolism , Lymph Nodes/cytology , Mice , Mice, Inbred C57BL , Tumor Cells, Cultured
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