Assessment of variations in productive performance of two different plumage color varieties of Japanese quail and their reciprocal crosses.

This study aimed to detect the phenotypic differences between the brown (BB) and white (WW) feathered quails and their reciprocal crosses (BW and WB) over two successive generations. The WW and cross quails, especially the BW, had the heaviest body weights, throughout the studied period, with significant variations between the two studied generations (P<0.05). Moreover, the WW and BW possessed the largest egg production during the F1, while in the F2, the BB had superiority among the studied quails with a prominent superiority of the F2 over the F1 (P<0.05). However, the F1 had higher egg weights than F2 with superiority of WW quails compared to the others (P<0.05). Also, the WW quails had the lowest lipid contents of the eggs. These phenotypic variations among the studied quails might be preliminarily explained by the results of the analyzed microsatellite markers despite the few markers used. The high variability among the BW and WB quails might be due to the larger number of alleles (NA and Ne) and the lower values of FIS with low heterozygosity levels (HO and He). Moreover, the BW and BB were the closest, while WB and WW were the farthest because of the high and low genetic identities and the high and low genetic distance between them, respectively. So the obtained results might introduce an initial scientific basis for evaluating and employing the genetic properties of BB, WW, BW, and WB quails in further genetic improvement program, and more microsatellite markers are recommended.

Mitigation of Imidacloprid Toxicity in Poultry Chicken by Selenium Nanoparticles: Growth Performance, Lipid Peroxidation, and Blood Traits.

Imidacloprid is an insecticide that protects against insects in the agriculture, animal, and poultry production sectors. Since the accumulation of imidacloprid induces adverse impacts on general health status and quality of the food chain, this study tested the impacts on broilers. Besides, selenium nanoparticles were fed to birds to relieve the negative impacts on growth performance and health status. Birds (1-day age, initial weight 46.05 ± 1.0 g) divided into four groups (triplicates) where 15 chicks of each replicate (45 for each group). The first group (control) was fed the basal diet without either selenium or imidacloprid toxicity. The second group was fed selenium nano form at 3 mg/kg. The third group was fed selenium and exposed to imidacloprid at 1/10 LT50 (3 mg/kg body weight). The fourth group was fed selenium nano form (3 mg/kg) and exposed to imidacloprid at 1/10 LT50 (3 mg/kg body weight). All groups were kept under the same conditions for 35 days. The final weight and weight gain of birds fed selenium nano form showed marked improvement compared to the imidacloprid-exposed group, while the feed intake and feed conversion ratio markedly reduced. The red blood cells showed higher values in birds fed selenium nano than the control and those exposed to imidacloprid. Interestingly, the hemoglobulin and hematocrit increased in birds fed selenium nano form with or without imidacloprid exposure. Furthermore, the white blood cells increased in birds fed selenium nano form with or without imidacloprid exposure. The total protein, albumin, and globulin were higher in birds fed selenium nanoparticles than those exposed to imidacloprid with or without selenium feeding. Birds in the control and imidacloprid groups had higher aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde levels than the remaining groups. Accordingly, dietary selenium nanoparticles are suggested in broiler feed to cope with the adverse effects of imidacloprid toxicity.

Comparing the Influences of Selenium Nanospheres, Sodium Selenite, and Biological Selenium on the Growth Performance, Blood Biochemistry, and Antioxidative Capacity of Growing Turkey Pullets.

Supplementation of selenium in poultry feed is required in an optimum dose and form for optimizing the growth performance and health status. Selenium nanospheres are suggested as an efficient and alternative to the conventional organic or inorganic forms. The study evaluated the effects of selenium (Se) nanospheres (SeNPs) as an alternative to organic Se (Sel-Plex®) or inorganic Se (sodium selenite, Se(IV Se(IV)) on the growth performance, carcass traits, blood biochemistry, and antioxidative capacity in turkey pullets. A total of 160 1-day-old Bronze turkey poults chicks were divided into four groups with 40 pullets each. The birds were fed on four types of diets as fellow: control (basal diet, 0.01 Se mg/kg), SeNPs (0.43 Se mg/kg), organic Se Sel-Plex® (0.41 Se mg/kg), and inorganic Se(IV) (0.42 Se mg/kg) for 8 weeks. No changes were seen in the body weight gain in growing turkey pullet, but chicks fed with Sel-Plex® form recorded the lowest feed intake (p < 0.05) compared to other treatments. Dietary SeNPs and Se(IV) selenium sources improved the feed conversion ratio compared to other treatments. All Se forms fed on turkey pullets showed higher carcass percentage weight and liver Se content than the control group. However, the gizzard percentage weight in the SeNPs group was lower than in the other treatments (p < 0.05). Birds fed SeNPs, and Sel-Plex® forms supplemental diets had a lower cholesterol concentration (p < 0.05) than the control and Se(IV). While high-density lipoprotein (HDL) concentration was increased in SeNPs and Se(IV) groups, and total protein concentration was higher in the Se(IV) group. Furthermore, dietary SeNPs reduced (p < 0.05) the low-density lipoprotein (LDL), total lipids, triglycerides, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine, uric acid, urea, and malondialdehyde plasma concentrations and increased the glutathione peroxidase activity (GPx) and total antioxidative capacity (TAC). In conclusion, the results confirmed that feeding turkey pullets on SeNPs form with the 0.4 Se mg/kg of feed enhanced feed efficiency, growth performance, carcass traits, plasma lipids concentration, and antioxidative capacity.

Planning for disposal of COVID-19 pandemic wastes in developing countries: a review of current challenges.

The health sector is critical to the well-being of any country, but developing countries have several obstacles that prevent them from providing adequate health care. This became an even larger concern after the COVID-19 outbreak left millions of people dead worldwide and generated huge amounts of infected or potentially infected wastes. The management and disposal of medical wastes during and post-COVID-19 represent a major challenge in all countries, but this challenge is particularly great for developing countries that do not have robust waste disposal infrastructure. The main problems in developing countries include inefficient treatment procedures, limited capacity of healthcare facilities, and improper waste disposal procedures. The management of medical wastes in most developing countries was primitive prior to the pandemic. The improper treatment and disposal of these wastes in our current situation may further speed COVID-19 spread, creating a serious risk for workers in the medical and sanitation fields, patients, and all of society. Therefore, there is a critical need to discuss emerging challenges in handling, treating, and disposing of medical wastes in developing countries during and after the COVID-19 outbreak. There is a need to determine best disposal techniques given the conditions and limitations under which developing countries operate. Several open questions need to be investigated concerning this global issue, such as to what extent developing countries can control the expected environmental impacts of COVID-19, particularly those related to medical wastes? What are the projected management scenarios for medical wastes under the COVID-19 outbreak? And what are the major environmental risks posed by contaminated wastes related to COVID-19 treatment? Studies directed at the questions above, careful planning, the use of large capacity mobile recycling facilities, and following established guidelines for disposal of medical wastes should reduce risk of COVID-19 spread in developing countries.

Nano-biofortification of different crops to immune against COVID-19: A review.

Human health and its improvement are the main target of several studies related to medical, agricultural and industrial sciences. The human health is the primary conclusion of many studies. The improving of human health may include supplying the people with enough and safe nutrients against malnutrition to fight against multiple diseases like COVID-19. Biofortification is a process by which the edible plants can be enriched with essential nutrients for human health against malnutrition. After the great success of biofortification approach in the human struggle against malnutrition, a new biotechnological tool in enriching the crops with essential nutrients in the form of nanoparticles to supplement human diet with balanced diet is called nano-biofortification. Nano biofortification can be achieved by applying the nano particles of essential nutrients (e.g., Cu, Fe, Se and Zn) foliar or their nano-fertilizers in soils or waters. Not all essential nutrients for human nutrition can be biofortified in the nano-form using all edible plants but there are several obstacles prevent this approach. These stumbling blocks are increased due to COVID-19 and its problems including the global trade, global breakdown between countries, and global crisis of food production. The main target of this review was to evaluate the nano-biofortification process and its using against malnutrition as a new approach in the era of COVID-19. This review also opens many questions, which are needed to be answered like is nano-biofortification a promising solution against malnutrition? Is COVID-19 will increase the global crisis of malnutrition? What is the best method of applied nano-nutrients to achieve nano-biofortification? What are the challenges of nano-biofortification during and post of the COVID-19?

Dietary Pomegranate By-Product Alleviated the Oxidative Stress Induced by Dexamethasone in Laying Hens in the Pre-Peak Period.

This experiment was conducted to assess the inclusion of the by-products of pomegranate peels to ameliorate the harmful impacts of oxidative stress in the pre-peak period of laying hens. For this, 120 local Egyptian strain hens (Inshas hens) aged 24 weeks old were used in four treatments. Pomegranate peel powder was included at 2% and 4% in the diets of laying hens subjected to oxidative injuries induced by dexamethasone compared with negative and positive control groups for 12 weeks. The addition of pomegranate peel powder (PPP) reduced the adverse effects of oxidative stress induced by dexamethasone on body weight (p = 0.006) and egg production (p = 0.010) comparing to the positive control. Additionally, pomegranate peel powder had a significant positive lowering effect on plasma cholesterol (p < 0.001) and triglyceride contents (p = 0.005) compared to control groups. The lipid peroxidation indicators (MDA) were reduced, but the antioxidative enzymes (SOD, CAT, and GPx) and total antioxidant blood capacity were improved with PPP. Based on the obtained data, the present research recommends using dietary PPP up to 4% to mitigate adverse oxidative stress effects in the pre-peak laying period and as a sustainable and economical approach for agricultural development.

Effect of feeding wheat middlings and calcium lignosulfonate as pellet binders on pellet quality growth performance and lipid peroxidation in broiler chickens.

Basal diets supplemented with 4 kg Ca-LS/ton of diet. Pellet quality characteristics (per cent fines, the present study was conducted to evaluate the influence of wheat middlings (WM) and calcium lignosulfonate (Ca-LS) as pellet binders on the pellet quality characteristics, growth performance, blood parameters, nutrients digestibilities, lipid peroxidation and muscle fatty acids profile in Egyptian broiler strain. A total of 3,120 broiler chicks at 1-day of age were divided randomly into three experimental treatments with eight replicates (130 each). The first treatment was fed the basal pelleted diets without any additives, the second treatment was fed diets including 50 kg WM/ton of diet and the third treatment was fed per cent pellets, and pellet durability index) were significantly improved in WM and Ca-LS treatments compared with the control. Body weight gain was significantly increased, while feed intake was significantly decreased resulting in improving of feed conversion ratio significantly in WM group in comparison with control and Ca-LS groups (p < .05). Nutrients apparent digestibility (dry matter, crude protein and crude fibre) were significantly improved by inclusion of WM compared with control and Ca-LS. Plasma total cholesterol, and uric acid concentrations were significantly decreased by dietary WM in comparison with control and Ca-LS experimental groups. Furthermore, linoleic, alpha-linolenic and arachidonic acids contents in breast muscle were significantly increased by WM and Ca-LS, while, muscle malondialdehyde concentration was significantly decreased. It could be concluded that inclusion of WM and Ca-LS can improve pellet quality characteristics, and WM (at a level of 50 kg/ton) had positive effects on growth performance, nutrients digestibilities, lipid peroxidation and fatty acids profile in Egyptian broiler strain.

The Influence of Dietary Chicken Egg Lysozyme on the Growth Performance, Blood Health, and Resistance Against Escherichia coli in the Growing Rabbits' Cecum.

The dietary chicken egg lysozyme (LZM) at different concentrations was tested on the growth performance, blood health, and resistance against Escherichia coli of growing rabbits. A total number of 48 rabbits averaged 611.25 g (5 weeks of age) of APRI line-rabbits (Egyptian developed line) were allocated into four treatments (three replicates and each contained four rabbits) of 5-week weaning APRI rabbits. The first group was fed a basal diet without LZM supplementation and served as a control group, whereas the remaining groups of rabbits were fed a basal diet supplemented with LZM at 50, 100, and 200 mg/kg diet, respectively, for 8 weeks. The obtained results revealed that rabbits fed the basal diet supplemented with different concentrations of LZM linearly (P < 0.05) displayed improved growth performance and reduced feed intake and FCR. The best result was for rabbits fed a 200 mg per kg diet supplemented with LZM, followed by a 100 mg per kg diet. The total count of Escherichia coli and Clostridium count was linearly (P < 0.05) decreased by adding LZM at 100 and 200 mg/kg in the diets compared to the control groups. In contrast, total bacterial count and the total count of Lactobacilli had increased considerably by increasing LZM at different levels relative to the control groups. The LZM supplementation linearly (P < 0.05) increased hematological parameters (RBCs, PCV, Hb, and WBCs) together with an increase in lymphocyte count compared to the control group. The total protein and globulin concentrations were significantly (P < 0.05) increased by feeding with LZM. On the other hand, ALT, AST, urea, and creatinine were significantly (P < 0.05) decreased by increasing LZM supplementation. It could be concluded that supplementation of the rabbit's diet with chicken egg LZM was able to improve the growth performance and hematological and serum biochemical parameters compared with the control group. Therefore, LZM is required at the rate of the hobx100-200 mg/kg diet as a potential feed additive and a friendly alternative for antibiotics in rabbit feed.

Knowledge and attitudes toward vaccination among Saudi medical students.

BACKGROUND: Studies have identified healthcare providers as an important determinant of vaccination acceptance. However, knowledge and attitudes toward vaccination have not been sufficiently studied in Saudi Arabia, especially among medical students. Therefore, we conducted this study to explore vaccination knowledge and attitudes among medical students at a large Saudi university. METHODS: A cross-sectional survey was conducted on 182 Saudi medical students between February 2019 and May 2019. Participants were invited to fill out a self-administered questionnaire assessing knowledge and attitudes toward vaccination. The statistical analysis included descriptive analysis, Chi-square test, independent samples t-test, and analysis of variance (ANOVA). The relationship between knowledge and attitudes was assessed using Pearson's correlation test. RESULTS: A total of 182 respondents completed the questionnaires, giving a response rate of 91%. The study included male (52.7%) and female (47.3%) students from study years 2, 3, 4, 5, and 6. The overall mean knowledge score was under average [3.05/9, standard deviation (SD) = 1.86] and the respondents showed generally moderate attitudes toward vaccination (mean = 30.60/45, SD = 6.07). While there was no sex difference in both the scores on knowledge and attitudes domains, the year of study was significantly associated with the mean knowledge score (F = 6.48, P < 0.01) and attitudes score (F = 7.12, P < 0.01). As predicted, there was a significant linear relationship between vaccination knowledge and attitudes (r = 0.71, P < 0.01). CONCLUSION: The study revealed generally moderate attitudes of Saudi medical students toward vaccination. However, several knowledge gaps were detected. The implications of the current findings are discussed.

Effect of dietary Moringa oleifera leaves on the performance, ileal microbiota and antioxidative status of broiler chickens.

In this study, we investigated the effect of Moringa oleifera leaves (MOL) supplements on the performance, ileal microbiota carcass traits and biochemical and antioxidant parameters of broiler chickens. MOL was added to starter, grower and finisher diets at four levels (0%, 0.5%, 1.0% and 5.0%). A total of 320 one-day-old mixed-sex Cobb-500 broiler chicks were divided equally into four treatment groups, with 5 replicates of 16 chicks each until 42 days of age. Compared to the control group, chicks fed 0.5% and 1% MOL exhibited a higher final body weight (FBW), greater weight gain (BWG), and better average daily gain (ADG). Feed intake (FI) gradually decreased as the inclusion rate of MOL in the broilers' diet increased to 5%. The best feed conversion ratio (FCR), protein efficiency ratio (PER) and performance index (PI) were observed in broilers that were fed the MOL diet. The percentage of abdominal fat in broilers decreased significantly in response to dietary supplementation with MOL. The haematological profile in the MOL group revealed a lower WBC count and lymphocyte percentage but higher heterophil and H/L ratio without affecting haemoglobin and RBC count. The inclusion of MOL increased total serum protein, Ca and P levels but decreased glucose, cholesterol, triglycerides, AST and ALT concentrations. Moreover, dietary MOL increased the activities of GSH, CAT and SOD, GPx and GST while decreasing the TBARS level. The pH value decreased in the ileum of broilers fed the 1% and 5% MOL diet compared to the other treatments. Decreased ileal counts of E. coli, Salmonella and Staphylococcus spp. were observed whereas the total ileal Lactobacillus spp. count increased in broilers fed the MOL diet. In conclusion, the MOL supplement can enhance growth performance and antioxidant status, alter ileal microbiota and protect against enteropathogens with no deleterious effects on the broiler chickens.

Feeding Aspergillus awamori reduces skeletal muscle protein breakdown and stimulates growth in broilers.

This study was conducted to show that dietary supplementation of a fungus, Aspergillus awamori called Koji in Japan, reduces skeletal muscle protein breakdown and stimulates growth in broiler chickens. A total of 30 chicks at 15 days of age was divided into control and two treatment groups (10 birds per treatment). Control group was fed basal diet and treatment groups were fed the basal diets supplemented with A. awamori at levels of 0.05% and 0.2%. The birds were raised for 12 days from 15 to 27 days of age and then the effect on growth, organ weights and plasma 3-methylhistidine concentration and digestibilities of protein and energy was evaluated. The messenger RNAs (mRNAs) of atrogin-1, ubiquitin, proteasome, m-calpain, µ-calpain, ß-actin, myosin and pax-7 in the breast muscle were also measured. Body weight gain and breast muscle weight were increased, although feed intake was decreased by the fungus and thus feed efficiency was increased. Protein and energy digestibilities were increased. Furthermore, plasma 3-methylhistidine concentration was decreased by the fungus. The mRNAs of atrogin-1, ubiquitin, proteasome, m-calpain and µ-calpain were all decreased. The mRNA of ß-actin but not myosin and pax-7 was slightly increased by the fungus. In conclusion, feeding A. awamori improves growth performance because skeletal muscle proteolytic activity is reduced and digestibilities of energy and protein are increased.

The modification of the muscle fatty acid profile by dietary supplementation with Aspergillus awamori in broiler chickens.

The present study was conducted to show that dietary supplementation with a fungus, Aspergillus awamori, modifies muscle fatty acid profiles in broiler chickens. A total of thirty chicks, selected from a group of 100 chicks aged 15 d, were divided into a control group and two treatment groups (ten birds per treatment). The control group was fed a basal diet, and the treatment groups were fed basal diets supplemented with A. awamori at levels of 0·05 and 0·2 %. From the start of the study at 15 d, the birds were raised for an additional 12 d, and growth and the muscle fatty acid profile were evaluated. Although feed intake was decreased by the fungus, body-weight gain and breast muscle weight were increased, and thus, feed efficiency was improved. Abdominal fat and plasma cholesterol and TAG were decreased, while plasma HDL-cholesterol and breast muscle fat content were increased. Interestingly, muscle α-tocopherol content was increased and muscle thiobarbituric acid-reactive substances were decreased by A. awamori. Furthermore, there was an observed decrease in SFA and an increase in unsaturated fatty acids in the muscle fat due to the fungus feeding. The mRNA of fatty acid synthase, acetyl-CoA carboxylase and Δ-6 desaturase in the muscle were all increased, while the mRNA of 3-hydroxyl-3-methylglutaryl-CoA reductase and carnitine palmitoyl transferase 1A were decreased by the fungus. In conclusion, the present study clearly shows that the muscle lipid profile could be modified by the addition of A. awamori to the diet.