Is a chlorhexidine reaction test better than dipsticks to detect asymptomatic bacteriuria in pregnancy?

Detection of asymptomatic bacteriuria (ASB) in pregnancy is important to avert the attendant morbidities. Therefore, we assessed the use of chlorhexidine reaction to detect ASB in pregnancy. This was a prospective study, which compared chlorhexidine reaction with dipstick tests and urine culture in 150 asymptomatic pregnant women. Urine cultures detected bacteriuria in seven women (4.7%). Chlorhexidine detected ASB in 72 women (48%) and had sensitivity, specificity and accuracy of 100%, 54% and 56%, respectively. Leucocyte esterase (LE) and nitrite detected bacteriuria in 31 (20.7%) women and 12 (8.0%) women, respectively. Singly, LE had a sensitivity and specificity of 14.3% and 79%, respectively, while nitrite's sensitivity and specificity was 42.9% and 93.7%, respectively. Combined, LE and nitrite had better sensitivity (97.9%) and accuracy (94%). Since the accuracy of chlorhexidine is low, other than urine culture, combined dipstick urinalysis of leucocyte esterase and nitrite tests is good to detect asymptomatic bacteriuria in pregnancy.

Seroprevalence and clinico-epidemiological correlates of hepatitis C viral antibodies at an antenatal booking clinic of a tertiary hospital in Nigeria.

PURPOSE: This was to determine the sero-prevalence of hepatitis C viral (HCV) antibodies in pregnant women attending the first antenatal clinic and assess the epidemiologic correlates of women anti-HCV positive. METHODS: This was a prospective observational study which used in vitro diagnostic test kits to detect anti-HCV antibodies. Women attending their first antenatal clinic were recruited at the antenatal clinic of Irrua Specialist Teaching Hospital, Edo State, Nigeria. Seropositive women had liver enzymes assessed, and screening for hepatitis B surface antigen and Human Immuno-deficiency Virus (HIV) was done. RESULTS: Eight out of 205 women were anti-HCV positive. The prevalence of hepatitis C infection was 3.9 %. The mean age of the women was 28.9 ± 2.1 years. Most (50 %) anti-HCV positive women had tertiary level education. Though health workers made up 3.5 % of the participants, they constituted 25 % women with anti-HCV antibody. Awareness of HCV infection had no impact on the rate of infection. Multiple sexual partners (P = 0.71), blood transfusion (0.64) and female circumcision (P = 1.00) were not significant risks of infection. 2 (1 %) women had hepatitis B co-infection and 1 (12.5 %) woman had both HCV antibody and HIV co-infection. CONCLUSION: Despite the 3.9 % prevalence, routine screening for hepatitis C virus infection in pregnancy is unjustified. Risk-based screening using locally prevailing risk factors with antenatal monitoring and postpartum treatment of women with hepatitis C antibodies is recommended.

Enhanced urinalysis in the detection of asymptomatic bacteriuria in pregnancy.

BACKGROUND: Detection and treatment of asymptomatic bacteriuria (ASB) in pregnancy is important to avert the attendant maternal and fetal morbidity. Other than urine culture, no other screening test is unequivocal. OBJECTIVE: The use of enhanced urinalysis test to detect ASB in pregnancy was investigated. METHODS: This was a prospective observational study which compared enhanced urinalysis with dipstick tests and urine culture. Clean catch midstream urine specimen was collected from 150 consecutive asymptomatic pregnant women. Tests of validity were used for comparison. RESULTS: Enhanced urinalysis detected bacteriuria as much as urine culture (4% vs. 4.7%). Itwas 57.1% sensitive and 98.6% specific. It had a false negative rate of 42.9% and was 96.7% accurate when compared to urine culture. Enhanced urinalysis took 1-2 hours to be done and required skills to use the microscope and was more expensive than dipstick urinalysis. CONCLUSION: The accuracy of enhanced urinalysis and its ability to detect ASB as much as urine culture connotes that it can be used to detect asymptomatic bacteriuria in pregnancy albeit only in secondary and tertiary health centres because of the cost and technicality involved.

Determination of antioxidant status of pre-eclamptic and normotensive sub-rural Nigerian pregnant women at the Irrua Specialist Teaching Hospital, Irrua, Edo State.

OBJECTIVES: To determine the antioxidant levels of subrural Nigerian population where pre-eclampsia and eclampsia is the leading cause of maternal mortality. METHODS: Prospective case control study done at Department of Obstetrics and Gynaecology, Irrua Specialist Teaching Hospital Irrua, Edo State, Nigeria. Plasma level of vitamin C and E were evaluated in 80 pre-eclamptic patientswhich were compared with normotensive 80 pregnant women matched as controls. RESULTS: Pre-eclampsia was associated with significant reduction in levels of vitamin C and E (p < 0.05). However, the correlation between the blood pressure (severity) and reduction in antioxidants level was not statistically significant. CONCLUSION: Pre-eclampsia at Irrua in Nigeria is associated with significant reduction in plasma antioxidants level similar to some reports from the other parts of the world.

Vasectomy: a survey of attitudes, counseling patterns and acceptance among Nigerian resident gynaecologists.

OBJECTIVES: Previous Nigerian studies show widespread ignorance and low acceptance of vasectomy among the male population. The objectives of this study were to determine the level of knowledge of, attitudes to, counselling pattern and acceptance of vasectomy among Nigerian Resident Gynaecologists. DESIGN: A cross-sectional questionnaire based survey. METHOD: Resident Doctors attending a national update course in obstetrics and gynaecology. RESULTS: Most of the doctors had good knowledge of Vasectomy. More than four-fifth of the doctors were convinced that the average Nigerian male will not accept vasectomy when indicated while more than three-fifth consider BTL a more appropriate option for permanent contraception in our setting. Forty one point three percent of the doctors will opt for vasectomy or urge their husbands to. Reasons for opposition to vasectomy were socio-cultural (21.3%), religious (13.1%) and psychological (41.0%), 24.6% had no specific reasons. While 89.4% of the doctors counselled often for BTL only 5.8% did for vasectomy. CONCLUSION: The Doctors showed good knowledge of vasectomy but most were poorly disposed towards use of vasectomy. The findings suggest a need for effective national training programmes targeted at resident doctors to enhance their knowledge of vasectomy as well as break barriers to personal use of, and counselling for vasectomy.

Retained Placenta: Still a Cause of Maternal Morbidity and Mortality in a Nigerian Semi- Urban Population

Background: Retained placenta is associated with morbidity and mortality when left untreated. This study was done to determine the occurrence of retained placenta in our setting as well as to ascertain the possible risk factors; morbidities and mortality. Method of study: This was a retrospective review of all cases of retained placenta over a three year period (March 2005 to March 2007). There were 3542 deliveries; and of which 64 cases were of retained placenta. Results: The incidence of retained placenta was 1.8of all deliveries with a higher incidence in unbooked patients and a case fatality of 3.12.The commonest complication was postpartum haemorrhage in 51(79.68) of cases with blood transfusion rate of 47. onclusion: Complications associated with retained placenta could be reduced by adequate utilisation of health care facilities manned by skilled attendants; availability of blood transfusion services as well as effective and safe anaesthesia

Eclampsia in Irrua Specialist Teaching Hospital: a five-year review.

OBJECTIVE: The contribution of Eclampsia to maternal mortality in Nigeria is well documented. In Irrua it accounts for over 25% of maternal deaths. Addressing Eclampsia is therefore a priority programme of the Irrua Safe motherhood Initiative. AIM: The aim of this study is to determine the incidence of eclampsia, its clinical correlates and outcome. Finally strategies will formulate programme implementation. MATERIALS AND METHODS: This is a retrospective study of cases of eclampsia in Irrua Specialist Teaching Hospital, Edo state; over a five-year period. Information was retrieved using a structured proforma. Statistical analysis was done using the excel statistical package and the Epi info 2002 statistical software. RESULTS: Seventy eight (78) were admitted in the 5 year study. This accounted for 2.52% of total labour ward admission. However only 74 case notes were available for analysis. 70/74 of the patients were unbooked emergencies. Among the unbooked patients. 38/70 (54.29%) of the unbooked had not received any antenatal care whatsoever. The incidence was disproportionately higher in younger women and teenagers as well as in primigravida. 55/74 (74.32%) had preceding headache while 21.62% had a prior history of blurred vision, 14.86% had epigastric pain.16.22% had restlessness while 10.81% had nausea and vomiting each. The perinatal mortality rate was 28.38% while the maternal mortality ratio was 22.97%. Maternal mortality was associated with poor urinary output (0.0003), history of native medication (0.0199), number of fits (0.00209). Platelet count below 50,000/ml, highest systolic blood pressure above 200 mmhg (0.000018) and pulmonary oedema (0.000558). CONCLUSION: Addressing Eclampsia in Irrua will include community campaigns, capacity building, retraining of staffs within the hospital and improved facilities for the management of cases.

Comparative study of intradermal smear microscopy in the diagnosis of malaria in symptomatic pregnant women.

OBJECTIVES: A comparative study of intradermal smear in the diagnosis of malaria in symptomatic pregnant women. Venous blood served as the control. PATIENTS AND METHODS: Fifty consecutive symptomatic pregnant women were recruited. Thick films of both venous and intradermal blood were examined. Questionnaires were used to determine patients' preference for the two techniques. Tests of statistical significance were done with Fisher exact and Yates correlation coefficient at 95% confidence interval. Sensitivity specificity and accuracy rates were used to assess the validity of intradermal smear. RESULTS: Intradermal smear more frequently diagnosed malaria parasitaemia than peripheral venous blood (66% vs 56%). This was statistically significant (P value: 0.0065). The sensitivity of intradermal smear was 85.7% while the positive predictive value was 77.4%. The accuracy rate was 76.7%. The technique of intradermal blood collection was preferred by 28% of women. CONCLUSION: Intradermal smear is useful in malaria diagnosis in pregnancy and may be an additional evaluation tool for persistent fever in pregnancy.

Myomectomy during second trimester pregnancy: a case report.

The prevalence of leiomyoma during pregnancy is reported as approximately 2%. Although during pregnancy leiomyomas usually remain asymptomatic, they may complicate its course. The management of leiomyoma during pregnancy is medical, but in certain circumstances surgical intervention and myomectomy may be required. This 28-year-old primigravida presented with progressively worsening lower abdominal pain at 14 weeks gestational age. Ultrasonography demonstrated a large fundally sited multi lobulated fibroid extending up to the right hypochondrium. There was an intrauterine gestation compatible with her menstrual dates. Laparotomy confirmed a large leiomyoma showing benign degenerative changes. The operative procedure was successful, and the pregnancy progressed normally. She was admitted at 34 weeks gestation with a diagnosis of antepartum haemorrhage. She was managed conservatively in the ward until 37 completed weeks gestation when she had an elective caesarean section and delivered of a live male neonate with a birth weight of 2.7 kg. She had type III placenta praevia.

Comparative Study of Intradermal Smear Microscopy in the Diagnosis of Malaria in Symptomatic Pregnant Women

Objectives: A comparative study of intradermal smear in the diagnosis of malaria in symptomatic pregnant women. Venous blood served as the control. Patients and methods : Fifty consecutive symptomatic pregnant women were recruited. Thick films of both venous and intradermal blood were examined. Questionnaires were used to determine patients' preference for the two techniques. Tests of statistical significance were done with Fisher exact and Yates correlation coefficient at 95confidence interval. Sensitivity specificity and accuracy rates were used to assess the validity of intradermal smear. Results: Intradermal smear more frequently diagnosed malaria parasitaemia than peripheral venous blood (66vs 56). This was statistically significant (P value: 0.0065). The sensitivity of intradermal smear was 85.7while the positive predictive value was 77.4. The accuracy rate was 76.7. The technique of intradermal blood collection was preferred by 28of women. Conclusion: Intradermal smear is useful in malaria diagnosis in pregnancy and may be an additional evaluation tool for persistent fever in pregnancy

Utility of intradermal blood smear in the detection of asymptomatic malaria parasitaemia in pregnancy.

OBJECTIVES: We evaluated the usefulness of intradermal smear microscopy (IDS) in the detection of asymptomatic malaria parasitaemia in pregnancy. Peripheral venous blood (PVB) served as control. The preference for the collection technique of dermal blood was also assessed. PATIENTS AND METHODS: One hundred and fifty (150) asymptomatic women were recruited. They had both intradermal smear and peripheral venous blood smear were made for all patients. Measures of test validity included sensitivity, specificity and accuracy rate. Test of statistical significance was with Yates correlation at 95% confidence limit. RESULTS: The prevalence of asymptomatic malaria parasitaemia was higher using intradermal smear (35.3% vs 33.3%) though this was not statistically significant. Intradermal smear had a sensitivity of 40% and specificity of 67%. The positive predictive value was 37.8% with accuracy rate of 58%. 41% of participants preferred the technique of collection of intradermal blood. CONCLUSION: Intradermal smear appears to have no usefulness in the detection of asymptomatic malaria parasitaemia in pregnancy. However, we recommend more studies on its value in pregnancy, especially amongst symptomatic pregnant women.

Chronic uterine inversion secondary to submucous fibroid: a case report.

A rare case of non-puerperal chronic uterine inversion secondary to sub mucous fibroid in a 38-year-old woman is presented. There was complete uterine inversion with the incarcerated inverted uterus protruding through the vvgina beyond the vulva. The sub mucous fibroid was attached to the fundus. At laparotomy, a dimple with a constriction ring was found in the position of the uterus. The distal ends of the fallopian tubes and part of the ovary were visible through the constriction ring. Histological examination of the uterus and fibroid following hysterectomy confirmed their benign nature.

The diagnostic accuracy of the rapid dipstick test to predict asymptomatic urinary tract infection of pregnancy.

Untreated urinary tract infection can have devastating maternal and neonatal effects. Thus, routine screening for bacteriuria is advocated. This study was designed to evaluate the diagnostic accuracy of the rapid dipstick test to predict urinary tract infection in pregnancy with the gold standard of urine microscopy, culture and sensitivity acting as the control. The urine dipstick test uses the leucocyte esterase, nitrite and test for protein singly and in combination. The result of the dipstick was compared with the gold standard, urine microscopy, culture and sensitivity using confidence interval for proportions. The reliability and validity of the urine dipstick was also evaluated. Overall, the urine dipstick test has a poor correlation with urine culture (p = 0.125, CI 95%). The same holds true for individual components of the dipstick test. The overall sensitivity of the urine dipstick test was poor at 2.3%. Individual sensitivity of the various components varied between 9.1% for leucocyte esterase and the nitrite test to 56.8% for leucocyte esterase alone. The other components of the dipstick test, the test of nitrite, test for protein and combination of the test (leucocyte esterase, nitrite and proteinuria) appear to decrease the sensitivity of the leucocyte esterase test alone. The ability of the urine dipstick test to correctly rule out urinary tract infection (specificity) was high. The positive predictive value for the dipstick test was high, with the leucocyte esterase test having the highest positive predictive value compared with the other components of the dipstick test. The negative predictive value (NPV) was expectedly highest for the leucocyte esterase test alone with values higher than the other components of the urine dipstick test singly and in various combinations. Compared with the other parameters of the urine dipstick test, singly and in combination, leucocyte esterase appears to be the most accurate (90.25%). The dipstick test has a limited use in screening for asymptomatic bacteriuria. The leucocyte esterase test component of the dipstick test appears to have the highest reliability and validity. The other parameters of the dipstick test decreases the reliability and validity of the leucocyte esterase test. A positive test merits empirical antibiotics, while a negative test is an indication for urine culture. The urine dipstick test if positive will also be useful in follow-up of patient after treatment of urinary tract infection. This is useful in poor resource setting especially in the third world where there is a dearth of trained personnel and equipment for urine culture.

Accuracy of urine dipsticks, 2-h and 12-h urine collections for protein measurement as compared with the 24-h collection.

The presence of protein in hypertensive disorders of pregnancy is a sign of a worsening condition and thus requires early intervention to prevent adverse consequences. Accurate assessment of proteinuria in patients with pre-eclampsia will ensure prompt and timely intervention to reduce or prevent the maternal and perinatal morbidity and mortality associated with pre-eclampsia. This study compared the reliability and validity of the more rapid diagnostic tests, such as the dipstick, 2-h and 12-h protein estimations with the 24-h protein. The result of the dipstick, 2-h and 12-h urine were also compared with the 24-h urine results using confidence interval (CI) for proportions with a value of p < 0.05 considered significant (CI 95%). When compared with the gold standard, there was a high degree of correlation between the 2-h (p = 0.244, CI 95%) and 12-h (p < 0.0255, CI 95%) with the 24-h sample in the quantification of proteinuria in women with pre-eclampsia. The most sensitive and specific test was the 12-h protein estimation, (89%) and (93%), respectively. The least sensitive and specific test was the dipstick test; (81%) and (47%), respectively. The 12-h protein estimation test had the highest positive predictive value (84%). The 12-h protein test also had the lowest false positive rate (12%) and false negative rates (11%), respectively. The most accurate test was the 12-h protein estimation (88%). The dipstick tests were however much cheaper and the results were faster. It is recommended that routine rapid quantisation of proteinuria in patients with pre-eclampsia be done using either the 2-h or 12-h urine sample.

Heterotropic pregnancy: a report of two cases.

Heterotropic pregnancy is reported infrequently in Nigeria. Two cases were managed within the span of five years with the successful delivery of a live term baby in one of the patients. A high clinical index of suspicion is required because an intra uterine gestation does not rule out a co-existing ectopic gestation especially in this region with high twinning rate and tubal disease

The demographic characteristics and health seeking behaviour of unbooked patients in Irrua Specialist Teaching Hospital.

BACKGROUND: The Obstetric outcome of the patients who receive antenatal care and deliver at the Irrua Specialist Teaching Hospital is reasonably satisfactory. Our major challenges arise from unbooked emergencies. The aim of this study was to determine the frequency of unbooked patients in the Irrua Specialist Teaching Hospital, their clinical presentation, their demographic characteristics as well as their obstetric outcome. METHODS: A structured proforma was used to collect relevant information over a one-year period from patients' case notes, theatre records and labour ward records. RESULTS: During the study period, unbooked patients constituted 14.7% of all deliveries. They had a higher proportion of teenagers (p < 0.0000) and women over 40 years (p < 0.0000) when compared to the booked patients. There were also a higher proportion of primigravidas and grandmultiparous women but these did not reach statistical significance. Unbooked patients are also more likely to be single (p < 0.001) and polygamous (p < 0.0002) when compared to the booked patients. The diagnosis on admission included obstructed labour (18.2%), intrauterine fetal death (14.9%), ante partum haemorrhage (12.4%), post date (12.4%) and eclampsia (8.3%). Eighteen (14.9%) of the unbooked patients had no antenatal care whatsoever, while sixteen 13.2% had been visiting TBAs for some care in pregnancy. Maternal mortality for the unbooked patients was 5/121 (4.1%). There was no maternal death amongst booked patients during the study. CONCLUSION: The unbooked patients are relatively high-risk patients with some social disadvantage. They have a high maternal mortality.

The Demographic Characteristics and Health Seeking Behaviour of Unbooked Patients in Irrua Specialist Teaching Hospital

Background: The Obstetric outcome of the patients who receive antenatal care and deliver at the Irrua Specialist Teaching Hospital is reasonably satisfactory. Our major challenges arise from unbooked emergencies. The aim of this study was to determine the frequency of unbooked patients in the Irrua Specialist Teaching Hospital; their clinical presentation; their demographic characteristics as well as their obstetric outcome. Methods: A structured proforma was used to collect relevant information over a one-year period from patients' case notes; theatre records and labour ward records. Results: During the study period; unbooked patients constituted 14.7of all deliveries. They had a higher proportion of teenagers (p

The Demographic Characteristics and Health Seeking Behaviour of Unbooked Patients in Irrua Specialist Teaching Hospital

Background: The Obstetric outcome of the patients who receive antenatal care and deliver at the Irrua Specialist Teaching Hospital is reasonably satisfactory. Our major challenges arise from unbooked emergencies. The aim of this study was to determine the frequency of unbooked patients in the Irrua Specialist Teaching Hospital; their clinical presentation; their demographic characteristics as well as their obstetric outcome. Methods: A structured proforma was used to collect relevant information over a one-year period from patients' case notes; theatre records and labour ward records. Results: During the study period; unbooked patients constituted 14.7of all deliveries. They had a higher proportion of teenagers (p

How useful is the Helen Keller food frequency chart in the determination of the vitamin A status in pregnancy?

Vitamin A deficiency sub clinical or overt, is associated with adverse maternal, fetal and neonatal outcome. This is also true for an excess of vitamin A. The challenge in pregnancy is to detect sub clinical vitamin A deficiency in patients for whom supplements or dietary manipulation will be of benefit. This was a cross sectional case controlled study at the University of Benin Teaching Hospital to compare the Helen Keller Food Frequency Chart with biochemical methods in the determination of vitamin A status in pregnancy. Data was collected from Antenatal patients (142). Using serum Biochemistry three categories of patient were recognized. Patients with normal vitamin A levels (N=100 women with blood vitamin A within two standard deviation of the mean) Twenty-four women (24) had low vitamin A levels (N=24, patients with blood vitamin A level at less than 2 standard deviation below the mean). Eighteen patients (18) had high vitamin A levels (patients with blood vitamin A levels at greater than two standard deviation above the mean). All recruited patients had a dietary assessment using the Helen Keller Food Frequency Chart. The Helen Keller Food Frequency Chart (HKFFC) was found to have a high degree of sensitivity (74.5%) and a high specificity (75%) in detection of patients with vitamin A deficiency. The positive predictive value was 93.62%. The low negative predictive rate of 37.5% however implies that a positive test is more important than a negative test. The HKFFC was unable to differentiate patients with normal or high vitamin A levels. Dietary assessment with the HKFFC is a cheap effective method to detect sub clinical vitamin A deficiency in pregnancy. It is an easy cost effective screening tool to select patients for whom dietary manipulation and or vitamin A supplementation may be beneficial.