ABSTRACT
Unusual behavior of chromium-51 in liquid scintillation cocktail is described. Rapidly declining count rate is attributed to first-order binding of chromate to glass vials.
Subject(s)
Chromium Radioisotopes/analysis , Binding Sites , Glass , Half-Life , Indicators and Reagents , Scintillation Counting/methodsSubject(s)
Anti-Inflammatory Agents/isolation & purification , Plants, Medicinal/analysis , Adrenocorticotropic Hormone/therapeutic use , Animals , Anti-Inflammatory Agents/analysis , Anti-Inflammatory Agents/therapeutic use , Anti-Inflammatory Agents/toxicity , Aspirin/therapeutic use , Chromatography , Colorimetry , Dexamethasone/therapeutic use , Edema/drug therapy , Electrophoresis, Disc , Female , Hydrocortisone/therapeutic use , Indomethacin/therapeutic use , Methods , Mice , Phenylbutazone/therapeutic useABSTRACT
The combustion method has been modified to increase the recovery of tritiated water after combustion of a tritium-labeled tissue sample. This was accomplished by cooling the bottom of the combustion flask in a dry ice-acetone bath while irradiating the top with an infrared lamp. The procedure resulted in at least 92% to 102% recovery of the tritiated water. The NCS solubilizer was found to be superior to hyamine for solubilizing(14)C labeled tissue samples. The samples yielded light yellow-colored solutions when incubated for 15 hr at 50-55C. The counting efficiency of this solution was 75% or higher.
