Petrographic and spectroscopic characterization of phosphate-stabilized mine tailings from Leadville, Colorado.

The use of soluble PO4(3-) and lime as a heavy metal chemical stabilization agent was evaluated for mine tailings from Leadville, Colorado. The tailings are from piles associated with the Wolftone and Maid of Erin mines; ore material that was originally mined around 1900, reprocessed in the 1940s, and now requires stabilization. The dominant minerals in the tailings are galena (PbS), cerrusite (PbCO3), pyromorphite (Pb5(PO4)3Cl), plumbojarosite (Pb0.5Fe3(SO4)2(OH)6), and chalcophanites ((Pb,Fe,Zn,Mn)Mn2O5 x 2H2O). The tailings were treated with soluble PO4(3-) and lime to convert soluble heavy metals (principally Pb, Zn, Cu, Cd) into insoluble metal phosphate precipitates. The treatment process caused bulk mineralogical transformations as well as the formation of a reaction rind around the particles dominated by Ca and P. Within the mineral grains, Fe-Pb phosphosulfates, Fe-Pb sulfates (plumbojarosite), and galena convert to Fe-Ca-Pb hydroxides. The Mn-Pb hydroxides and Mn-(+/-Fe)-Pb hydroxides (chalcophanites) undergo chemical alteration throughout the grains during treatment. Bulk and surface spectroscopies showed that the insoluble reaction products in the rind are tertiary metal phosphate (e.g. (Cu,Ca2)(PO4)2) and apatite (e.g. Pb5(PO4)3Cl) family minerals. pH-dependent leaching (pH 4,6,8) showed that the treatment was able to reduce equilibrium concentrations by factors of 3 to 150 for many metals; particularly Pb2+, Zn2+, Cd2+, and Cu2+. Geochemical thermodynamic equilibrium modeling showed that apatite family and tertiary metal phosphate phases act as controlling solids for the equilibrium concentrations of Ca2+, PO4(3-) Pb2+, Zn2+, Cd2+, and Cu2+ in the leachates during pH-dependent leaching. Both end members and ideal solid solutions were seen to be controlling solids.

Effect of reactor turbulence on the binding-protein-mediated aspartate transport system in thin wastewater biofilms.

This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport l-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport systems (systems 1 and 2) were subjected to a turbulent flow condition in a hydrodynamically defined closed-loop reactor system. A shear stress treatment of 3.1 N . m for 10 min at a turbulent Reynolds number (Re = 11,297) inactivated the low-affinity, high-capacity binding-protein-mediated transport system (system 2) and resolved the high-affinity, low-capacity membrane-bound proton symport system (system 1). The K(t) and V(max) values for the resolved system were statistically similar to K(t) and V(max) values for system 1 when system 2 was inactivated either by osmotic shock or arsenate, two treatments which are known to inactivate binding-protein-mediated transport systems. We hypothesize that shear stress disrupts system 2 by deforming the outer membranes of the firmly adhered gram-negative bacteria.

Multiplicity of aspartate transport in thin wastewater biofilms.

This research documents the multiplicity of L-aspartate transport in thin wastewater biofilms. A Line-weaver-Burk analysis of incorporation produced a curvilinear plot (concave down) that suggested active transport by two distinct systems (1 and 2). The inactivation of system 2 with AsO4 or osmotic shock resolved system 1, which was a high-affinity, low-capacity system with an apparent Kt (Michaelis-Menten constant) of 4.3 microM (AsO4) or 4.6 microM (osmotic shock). The inactivation of system 1 with dinitrophenol resolved system 2, which was a low-affinity, high-capacity system with an apparent Kt of 116.7 microM. System 1 was more specific for aspartate than system 2 in the presence of aspartate analogs. Sodium had no discernible effect on the incorporation velocities by either system. These results indicate that system 1 is a membrane-bound proton symport coupled to the proton gradient component of the proton motive force and that system 2 is a binding protein-mediated system coupled to phosphate bond energy. Analyses of diffusional limitations on the derived transport constants indicated that internal resistances were present but that the apparent constants were close to the intrinsic values, especially for system 1. Metabolic inactivation of the biofilm with dinitrophenol and AsO4 did not completely inactivate aspartate incorporation, which indicated that some simple adsorption of the aspartate anion by the biofilm had occurred. These results show that aspartate is transported by wastewater biofilm bacteria via systems with different affinities, specificities, and mechanisms of energy coupling.

Electron microscopic examination of wastewater biofilm formation and structural components.

This research documents in situ wastewater biofilm formation, structure, and physiochemical properties as revealed by scanning and transmission electron microscopy. Cationized ferritin was used to label anionic sites of the biofilm glycocalyx for viewing in thin section. Wastewater biofilm formation paralleled the processes involved in marine biofilm formation. Scanning electron microscopy revealed a dramatic increase in cell colonization and growth over a 144-h period. Constituents included a variety of actively dividing morphological types. Many of the colonizing bacteria were flagellated. Filaments were seen after primary colonization of the surface. Transmission electron microscopy revealed a dominant gram-negative cell wall structure in the biofilm constituents. At least three types of glycocalyces were observed. The predominant glycocalyx possessed interstices and was densely labeled with cationized ferritin. Two of the glycocalyces appeared to mediate biofilm adhesion to the substratum. The results suggest that the predominant glycocalyx of this thin wastewater biofilm serves, in part, to: (i) enclose the bacteria in a matrix and anchor the biofilm to the substratum and (ii) provide an extensive surface area with polyanionic properties.