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1.
Sci Rep ; 9(1): 15872, 2019 Oct 29.
Article in English | MEDLINE | ID: mdl-31659199

ABSTRACT

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

2.
Sci Rep ; 9(1): 11943, 2019 08 16.
Article in English | MEDLINE | ID: mdl-31420565

ABSTRACT

We investigated three bovine respiratory pathobionts in healthy cattle using qPCR optimised and validated to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynamic range. A longitudinal study was conducted to investigate the carriage and density of these bacteria in the nasal passages of healthy beef calves (N = 60) housed over winter in an experimental farm setting. The three pathobiont species exhibited remarkably different carriage rates and density profiles. At housing, high carriage rates were observed for P. multocida (95%), and H. somni (75%), while fewer calves were positive for M. haemolytica (13%). Carriage rates for all three bacterial species declined over the 75-day study, but not all individuals became colonised despite sharing of environment and airspace. Colonisation patterns ranged from continuous to intermittent and were different among pathobiont species. Interval-censored exponential survival models estimated the median duration of H. somni and P. multocida carriage at 14.8 (CI95%: 10.6-20.9) and 55.5 (CI95%: 43.3-71.3) days respectively, and found higher density P. multocida carriage was associated with slower clearance (p = 0.036). This work offers insights into the dynamics of pathobiont carriage and provides a potential platform for further data collection and modelling studies.


Subject(s)
Cattle Diseases/microbiology , Mannheimia haemolytica/growth & development , Pasteurella multocida/growth & development , Pasteurellaceae Infections/microbiology , Pasteurellaceae Infections/veterinary , Pasteurellaceae/growth & development , Animals , Bacterial Load , Carrier State , Cattle , Cattle Diseases/diagnosis , DNA, Bacterial/genetics , Longitudinal Studies , Male , Mannheimia haemolytica/classification , Mannheimia haemolytica/isolation & purification , Nasal Cavity/microbiology , Pasteurella multocida/classification , Pasteurella multocida/isolation & purification , Pasteurellaceae/classification , Pasteurellaceae/genetics , Pasteurellaceae/isolation & purification , Pasteurellaceae Infections/diagnosis , Polymerase Chain Reaction
3.
J Helminthol ; 92(2): 128-141, 2018 Mar.
Article in English | MEDLINE | ID: mdl-28528590

ABSTRACT

Trematode infections cause serious economic losses to livestock worldwide. Global production losses due to fasciolosis alone exceed US$3 billion annually. Many trematode infections are also zoonotic and thus a public health concern. The World Health Organization has estimated that about 56 million people worldwide are infected by at least one zoonotic trematode species, and up to 750 million people are at risk of infection. Fasciolosis caused by the fluke Fasciola gigantica is endemic in Nigeria and is one of the most common causes of liver condemnation in abattoirs. Total cattle losses from Fasciola infection in Nigeria have been estimated to cost £32.5 million. Other trematode infections of cattle, including paramphistomosis, dicrocoeliasis and schistosomiasis, have all been reported in various parts of Nigeria, with varying prevalence. Most publications on trematode infections are limited to Nigerian local and national journals, with very few international reports. This paper therefore summarized the current data on distribution, control and zoonotic trematode infections in Nigeria and other African countries. We also identified research gaps and made recommendations for future research and areas for funding for policy/planning.


Subject(s)
Cattle Diseases/epidemiology , Fascioliasis/epidemiology , Fascioliasis/veterinary , Trematode Infections/veterinary , Abattoirs , Africa/epidemiology , Animals , Cattle/parasitology , Cattle Diseases/economics , Cattle Diseases/parasitology , Fasciola/isolation & purification , Fascioliasis/parasitology , Liver/parasitology , Nigeria/epidemiology , Prevalence , Trematode Infections/epidemiology , Trematode Infections/parasitology , Zoonoses/economics , Zoonoses/epidemiology , Zoonoses/parasitology , Zoonoses/transmission
4.
Vet Rec ; 181(11): 298-299, 2017 Sep 16.
Article in English | MEDLINE | ID: mdl-28916694

ABSTRACT

The Royal College of Veterinary Surgeons now lists 'How to evaluate evidence' as a day one competence for newly qualified vets. In this article, representatives from each of the veterinary schools in the UK discuss how the challenge of delivering and assessing the concepts of evidence-based veterinary medicine in a crowded undergraduate curriculum can be met.


Subject(s)
Education, Veterinary/organization & administration , Evidence-Based Medicine/education , Teaching/psychology , Curriculum , Humans , Schools, Veterinary , United Kingdom
6.
Exp Appl Acarol ; 55(2): 203-13, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21499913

ABSTRACT

Tick abundance and seroconversion rates of 640 indigenous cattle in a mixed crop-livestock system in Uganda were investigated in a 14 months longitudinal study. Up to 100% of the cattle in Buyimini, Kubo, Nanjeho, Ojilai and Sitengo villages (high tick challenge zone) were consistently infested with Rhipicephalus appendiculatus, whereas on average 50% of the cattle in Bunghaji, Hitunga and Magoje villages (low tick challenge zone) were inconsistently infested. Likewise, up to 50% of the cattle in Buyimini, Kubo, Nanjeho, Ojilai and Sitengo villages were consistently infested with R. (Boophilus) decoloratus ticks, while on average 30% of the cattle in Bunghaji, Hitunga and Magoje were inconsistently infested. Seroconversion rates of cattle to Anaplasma marginale infection under low tick challenge were higher than those under high tick challenge, but the reverse was true for Babesia bigemina infection. For Theileria parva infection, seroconversion rates of cattle older than 6 months under low tick challenge were significantly higher than those under high tick challenge (P < 0.05). However, the likelihood of occurrence of theileriosis cases among calves (0-6 m) under high tick challenge was 6 times (Odds ratio = 5.82 [1.30-36.37]) higher than under low tick challenge. The high density of anti-tick plants Lantana camara and Ocimum suave that were widespread in villages with low tick challenge, among other factors, was probably the cause for unfavourable tick survival.


Subject(s)
Arthropod Vectors , Cattle/parasitology , Tick Infestations/veterinary , Tick-Borne Diseases/veterinary , Ticks , Anaplasma marginale/immunology , Animals , Babesia/immunology , Cattle/immunology , Population Density , Theileria parva/immunology , Tick Infestations/epidemiology , Tick-Borne Diseases/epidemiology , Tick-Borne Diseases/transmission , Uganda
7.
Land use policy ; 27(3): 888-897, 2010 Jul.
Article in English | MEDLINE | ID: mdl-22210972

ABSTRACT

In developing countries, cities are rapidly expanding and urban and peri-urban agriculture (UPA) has an important role in feeding these growing urban populations; however such agriculture also carries public health risks such as zoonotic disease transmission. It is important to assess the role of UPA in food security and public health risks to make evidence-based decisions on policies. Describing and mapping the peri-urban interface (PUI) are the essential first steps for such an assessment. Kampala, the capital city of Uganda is a rapidly expanding city where the PUI has not previously been mapped or properly described. In this paper we provide a spatial representation of the entire PUI of Kampala economic zone and determine the socio-economic factors related with peri-urbanicity using a population-dynamics focussed rapid rural mapping. This fills a technical gap of rapid rural mapping and offers a simple and rapid methodology for describing the PUI which can be applied in any city in developing countries for wide range of studies.

8.
Vet Parasitol ; 160(3-4): 301-5, 2009 Mar 23.
Article in English | MEDLINE | ID: mdl-19111994

ABSTRACT

Diagnosis of major endemic bovine parasitic diseases in sub-Saharan Africa such as trypanosomosis, theileriosis, anaplasmosis, babesiosis and cowdriosis is increasingly relying on clinical diagnosis due to deterioration of veterinary services and laboratory facilities. Pyrexia is a common clinical feature of aforementioned diseases whose detection relies on measurement of rectal temperature. The research undertaken in this study was aimed at assessing the effects of diurnal changes and variable coat colour of indigenous Nkedi Zebu cattle on the diagnostic value of rectal temperature under tropical conditions. The results revealed that variation in rectal temperature was significantly influenced by time of day it was taken and by the coat colour of the Nkedi Zebu cattle (P<0.001). Rectal temperature experienced diurnal changes: steadily rising to reach a peak at 17.00h before declining. The mean rectal temperature of unhealthy cattle was significantly higher (P<0.05) than that of the healthy ones only between 13.00 and 17.00h of the day. During which period the proportion of unhealthy cattle having a rectal temperature of 39.4 degrees C or higher was significantly higher than that of healthy ones (P<0.001). Regarding the variable coat colour of indigenous breeds, rectal temperature among cattle of different coat colours was significantly different (P<0.05). In conclusion it is important to consider diurnal changes in rectal temperature and differences due to variable coat colour of indigenous African breeds when measuring rectal temperature for assessing pyrexia, during clinical diagnosis of bovine trypanosomosis and tick-borne diseases that are endemic in many countries in sub-Saharan Africa.


Subject(s)
Body Temperature/physiology , Cattle Diseases/diagnosis , Tick Infestations/veterinary , Tick-Borne Diseases/veterinary , Trypanosomiasis/veterinary , Africa South of the Sahara/epidemiology , Animals , Cattle , Cattle Diseases/epidemiology , Cattle Diseases/genetics , Circadian Rhythm , Color , Female , Fever/diagnosis , Fever/epidemiology , Fever/veterinary , Hair , Host-Parasite Interactions , Pigmentation , Species Specificity , Tick Infestations/diagnosis , Tick Infestations/epidemiology , Tick-Borne Diseases/diagnosis , Tick-Borne Diseases/epidemiology , Ticks/parasitology , Trypanosoma , Trypanosomiasis/diagnosis , Trypanosomiasis/epidemiology
9.
Vet Parasitol ; 155(3-4): 273-80, 2008 Aug 17.
Article in English | MEDLINE | ID: mdl-18579313

ABSTRACT

A longitudinal study was conducted in Southeast Uganda for 14 months on 640 Zebu cattle kept under natural tick challenge, with a view to identifying clinical features for prediction of seroconversion to Anaplasma marginale, Babesia bigemina and Theileria parva infections. Physical examination, condition scoring and tick counts were undertaken on all cattle every 4 weeks. In addition, 5300 sera were collected and analysed for antibodies against A. marginale, B. bigemina and T. parva infections using the enzyme-linked immunosorbent assay (ELISA). The major clinical features compiled included weight loss, fever (rectal temperature), anaemia (packed cell volume), pallor of mucous membranes, lymph node enlargement, staring coat, diarrhoea and lacrymation. The risk factors included tick challenge at village level, sex, age, Rhipicephalus spp. density and Boophilus spp. density on individual animals. Using a binary logistic regression model, the clinical features and risk factors were analysed. The results suggest that increasing rectal temperature was associated with increased probability for seroconversion to A. marginale, while high level of Rhipicephalus spp. density and increasing packed cell volume (PCV) were significantly associated with reduced probability of seroconversion. Although statistically significant, none of the factors had large effects, with odds ratios (OR) of 0.87, 1.15 and 0.98 for Rhipicephalus spp. density, rectal temperature and PCV, respectively. For B. bigemina infection, a high level of Boophilus spp. density, anaemia and staring coat were significantly associated with increased probability of seroconversion (OR 1.50, 1.78, 1.37, respectively). Presence of lacrymation and old age were associated with reduced probability of seroconversion (OR 0.52, 0.86 respectively). For T. parva infection, lymph node enlargement (OR 1.30) was associated with increased probability of seroconversion, while high Rhipicephalus spp. density and increasing packed cell volume (PCV) were associated with reduced probability of seroconversion (OR 0.68 and 0.98, respectively). In conclusion, presence and intensity of the respective tick vectors for tick-borne diseases, age and clinical features such as anaemia, fever, staring coat, lymph node enlargement and lacrymation are indicators for seroconversion to A. marginale, B. bigemina and T. parva infections in cattle. These indicators for seroconversion could be exploited in the development of decision support tools for clinical diagnosis of tick-borne diseases.


Subject(s)
Anaplasma marginale/immunology , Antibodies, Bacterial/blood , Antibodies, Protozoan/blood , Babesia/immunology , Cattle Diseases/immunology , Theileria parva/immunology , Tick Infestations/veterinary , Anaplasmosis/blood , Anaplasmosis/immunology , Animals , Babesiosis/blood , Babesiosis/immunology , Babesiosis/veterinary , Cattle , Cattle Diseases/blood , Female , Logistic Models , Longitudinal Studies , Male , Risk Factors , Theileriasis/blood , Theileriasis/immunology , Tick Infestations/microbiology , Tick Infestations/parasitology , Tick Infestations/physiopathology , Uganda
11.
Epidemiol Infect ; 135(1): 67-75, 2007 Jan.
Article in English | MEDLINE | ID: mdl-16740196

ABSTRACT

Diagnosis and treatment of endemic infectious disease is crucial for productivity of cattle in rural sub-Saharan Africa, but shortages of trained veterinary professionals necessitate support for less well-trained cadres of animal health worker. A Delphi survey of veterinary experts provided quantitative information on key clinical signs associated with eight endemic bovine diseases, then heuristics and dendrogram analysis identified a reduced sign set to be incorporated in a diagnostic decision support tool implemented as a simple colour-banded card. One hundred and seventy disease-sign questionnaire returns were obtained from 32 veterinary research scientists and 14 veterinary practitioners. Preliminary validation of the decision support tool for 16 prototypical cases resulted in 'correct' diagnosis over 90% of the time. The card potentially serves as a training aid and aide-mémoire, and could improve the diagnostic competence of animal healthcare providers.


Subject(s)
Animals, Domestic , Cattle Diseases/diagnosis , Decision Support Techniques , Education, Veterinary/methods , Endemic Diseases/veterinary , Africa South of the Sahara , Animal Husbandry , Animals , Cattle , Cattle Diseases/epidemiology , Cattle Diseases/etiology , Cattle Diseases/physiopathology , Education, Veterinary/economics , Risk Factors , Severity of Illness Index , Surveys and Questionnaires , Veterinarians
12.
Acta Trop ; 97(2): 229-32, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16387279

ABSTRACT

We present the results of a study to determine the value of central point sampling in cattle markets as a means of estimating the trypanosomiasis (T. brucei s.l.) prevalence in the surrounding landscape in Uganda. We find that in the epidemic area studied, central point sampling is a good predictor of prevalence in surrounding villages, but not in endemic areas. We also find that animals infected with trypanosomiasis are more likely to be brought for sale in livestock markets in endemic areas; we discuss these results in relation to the prevention of the spread of sleeping sickness.


Subject(s)
Cattle Diseases/parasitology , Trypanosoma brucei rhodesiense/isolation & purification , Trypanosomiasis, African/veterinary , Animals , Antibodies, Protozoan/blood , Cattle , Cattle Diseases/epidemiology , Humans , Linear Models , Prevalence , Rural Population , Seroepidemiologic Studies , Trypanosomiasis, African/epidemiology , Trypanosomiasis, African/parasitology , Uganda/epidemiology
15.
Vet Parasitol ; 125(1-2): 163-81, 2004 Oct 28.
Article in English | MEDLINE | ID: mdl-15476966

ABSTRACT

This manuscript provides a summary of the results presented at a symposium organized to accumulate information on factors that influence the prevalence of acaricide resistance and tick-borne diseases. This symposium was part of the 19th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), held in New Orleans, LA, USA, during August 10-14, 2003. Populations of southern cattle ticks, Boophilus microplus, from Mexico have developed resistance to many classes of acaricide including chlorinated hydrocarbons (DDT), pyrethroids, organophosphates, and formamidines (amitraz). Target site mutations are the most common resistance mechanism observed, but there are examples of metabolic mechanisms. In many pyrethroid resistant strains, a single target site mutation on the Na(+) channel confers very high resistance (resistance ratios: >1000x) to both DDT and all pyrethroid acaricides. Acetylcholine esterase affinity for OPs is changed in resistant tick populations. A second mechanism of OP resistance is linked to cytochrome P450 monooxygenase activity. A PCR-based assay to detect a specific sodium channel gene mutation that is associated with resistance to permethrin has been developed. This assay can be performed on individual ticks at any life stage with results available in a few hours. A number of Mexican strains of B. microplus with varying profiles of pesticide resistance have been genotyped using this test. Additionally, a specific metabolic esterase with permethrin-hydrolyzing activity, CzEst9, has been purified and its gene coding region cloned. This esterase has been associated with high resistance to permethrin in one Mexican tick population. Work is continuing to clone specific acetylcholinesterase (AChE) and carboxylesterase genes that appear to be involved in resistance to organophosphates. Our ultimate goal is the design of a battery of DNA- or ELISA-based assays capable of rapidly genotyping individual ticks to obtain a comprehensive profile of their susceptibility to various pesticides. More outbreaks of clinical bovine babesisois and anaplasmosis have been associated with the presence of synthetic pyrethroid (SP) resistance when compared to OP and amidine resistance. This may be the result of differences in the temporal and geographic patterns of resistance development to the different acaricides. If acaricide resistance develops slowly, herd immunity may not be affected. The use of pesticides for the control of pests of cattle other than ticks can affect the incidence of tick resistance and tick-borne diseases. Simple analytical models of tick- and tsetse-borne diseases suggest that reducing the abundance of ticks, by treating cattle with pyrethroids for example, can have a variety of effects on tick-borne diseases. In the worst-case scenario, the models suggest that treating cattle might not only have no impact on trypanosomosis but could increase the incidence of tick-borne disease. In the best-case, treatment could reduce the incidence of both trypanosomosis and tick-borne diseases Surveys of beef and dairy properties in Queensland for which tick resistance to amitraz was known were intended to provide a clear understanding of the economic and management consequences resistance had on their properties. Farmers continued to use amitraz as the major acaricide for tick control after the diagnosis of resistance, although it was supplemented with moxidectin (dairy farms) or fluazuron, macrocyclic lactones or cypermethrin/chlorfenvinphos.


Subject(s)
Acaricides/pharmacology , Cattle Diseases/prevention & control , Cattle Diseases/parasitology , Insecticide Resistance , Tick-Borne Diseases/veterinary , Ticks/drug effects , Ticks/genetics , Animals , Cattle , Genotype , Mexico , Prevalence , Tick-Borne Diseases/parasitology
16.
Prev Vet Med ; 63(1-2): 29-38, 2004 Apr 30.
Article in English | MEDLINE | ID: mdl-15099714

ABSTRACT

In a tsetse-infested area of Kenya, we characterised veterinary-drug outlets based on personnel and business characteristics to assess their capacities to provide clinical veterinary services. Structured questionnaires were administered to the retailers and sought information on the characteristics of the owners, salespersons and businesses. A total of 41 retail outlets (20 agro-veterinary, 11 pharmacy, and 10 general shops) were identified. There was poor response to questions on owner characteristics. Proprietors, who had no more than secondary education owned 15 out of 28 shops. Few shops (4/29) were owned by proprietors, who had professional qualifications (in animal health). Most salespersons had only secondary education but no qualifications. Animal-health assistants (AHAs), veterinarians and manufacturer's package inserts (drug leaflets) were the preferred information sources for the retailers. We concluded that drug retailers were poorly equipped with the technical knowledge necessary for drug dispensation and advice.


Subject(s)
Commerce/education , Health Knowledge, Attitudes, Practice , Trypanosomiasis/prevention & control , Veterinary Drugs/supply & distribution , Animals , Commerce/legislation & jurisprudence , Consumer Product Safety/legislation & jurisprudence , Educational Status , Humans , Insect Vectors , Kenya , Ownership , Surveys and Questionnaires , Trypanosomiasis/drug therapy , Tsetse Flies
17.
Vet Parasitol ; 119(2-3): 125-35, 2004 Jan 30.
Article in English | MEDLINE | ID: mdl-14746972

ABSTRACT

A survey to investigate resistance to drugs used in the treatment of bovine trypanosomosis was conducted in the eastern province of Zambia between 1996 and 1998. A cross-sectional study was conducted in three districts (Petauke, Katete, Lundazi) at 34 village sampling sites selected at random from villages that had shown greater than 6% prevalence of bovine trypanosomosis during an earlier survey. A longitudinal study was conducted in same three districts over a 1-year period. The study sites were chosen from the cross-sectional study and included eight sites showing high trypanosomosis prevalence and where no control activities were recorded. Use was made of parasitological methods, tests of resistance in cattle and mice and isometamidium-ELISA. Overall mean prevalence of trypanosomosis was 14.4, with 96% of infections caused by Trypanosoma congolense. The remainder was caused by Trypanosoma vivax (2%) and Trypanosoma brucei (2%). Tests in mice showed that of the stabilates collected, 24 (34%) were resistant to only isometamidium chloride, 8 (11.3%) were resistant to only diminazene aceturate, 1 (1.4%) was resistant to both drugs, and 38 (53.5%) were sensitive to both drugs. At least 2 out of 27 stabilates tested in cattle appeared to be resistant to trypanocidal drugs, 1 to isometamidium and 1 to diminazene. Isometamidium could be detected in only 63 (4.1%) of 1526 serum samples from cattle in the study. Only 6 (2.8%) of 212 serum samples from trypanosome-infected cattle had serum levels of the drug above 0.4 ng isometamidium per ml serum which is indicative for drug resistance in the infecting parasite population. Although some drug resistance is apparent, diminazene aceturate and isometamidium chloride can still be expected to be effective as a sanative pair in this area in most cases, since not more than 1 stabilate of 71 investigated showed evidence of resistance to both drugs.


Subject(s)
Trypanosoma congolense/isolation & purification , Trypanosomiasis, Bovine/drug therapy , Animals , Antibodies, Protozoan/blood , Cattle , Cross-Sectional Studies , Drug Resistance/physiology , Enzyme-Linked Immunosorbent Assay/veterinary , In Vitro Techniques , Longitudinal Studies , Male , Mice , Prevalence , Seroepidemiologic Studies , Trypanocidal Agents/pharmacology , Trypanocidal Agents/therapeutic use , Trypanosomiasis, Bovine/blood , Trypanosomiasis, Bovine/epidemiology , Trypanosomiasis, Bovine/parasitology , Zambia/epidemiology
18.
Onderstepoort J Vet Res ; 70(3): 213-8, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14621317

ABSTRACT

The clinical, parasitological and molecular diagnosis of bovine trypanosomosis were compared using samples from 250 zebu cattle exposed to natural trypanosome challenge in Uganda. Clinical examination, molecular and parasitological diagnoses detected 184 (73.6%), 96 (38.4%) and 36 (14.4%) as diseased, respectively. The sensitivity and specificity of clinical examination were 87.5% and 35%, and 78 % and 27 % based on molecular and parasitological diagnoses, as gold standards, respectively. Of the 33, 3, 13 and 12 parasitological-positive cattle that had Trypanosoma brucei, Trypanosoma congolense, Trypanosoma vivax or mixed infections, 78 %, 33 %, 84 % and 100 % respectively manifested clinical signs. Of the 24, 89, 12, 3, 6 and 27 cattle detected by molecular diagnosis to have mixed infections, T. brucei, T. vivax, T. congolense forest-, Savannah- and Tsavo-type, 100%, 83%, 91%, 100%, 67% and 81 % had clinical signs, respectively. In conclusion, treatment of cattle based on clinical examination may clear up to 87.5 % or 78 % of the cases that would be positive by either molecular or parasitological diagnosis, respectively. Under field conditions, in the absence of simple and portable diagnostic tools or access to laboratory facilities, veterinarians could rely on clinical diagnosis to screen and treat cases of bovine trypanosomosis presented by farmers before confirmatory diagnosis in diagnostic centres for few unclear cases is sought.


Subject(s)
Trypanosoma/isolation & purification , Trypanosomiasis, Bovine/diagnosis , Animals , Cattle , DNA, Protozoan/blood , Diagnosis, Differential , Physical Examination/methods , Physical Examination/veterinary , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/veterinary , Prevalence , Sensitivity and Specificity , Trypanosoma/genetics , Trypanosoma brucei brucei/genetics , Trypanosoma brucei brucei/isolation & purification , Trypanosoma congolense/genetics , Trypanosoma congolense/isolation & purification , Trypanosoma vivax/genetics , Trypanosoma vivax/isolation & purification , Trypanosomiasis, Bovine/blood , Trypanosomiasis, Bovine/pathology , Uganda
19.
Vet Parasitol ; 110(1-2): 117-22, 2002 Dec 11.
Article in English | MEDLINE | ID: mdl-12446096

ABSTRACT

A survey to monitor the use of trypanocidal drugs by cattle breeders was conducted in Zambia. Use was made of a questionnaire and of the isometamidium-ELISA technique. One hundred and twenty-two farmers and 50 veterinary assistants were interviewed. The isometamidium-ELISA was used to monitor the isometamidium serum concentration in 72 cattle, 1 week after unsupervised treatment by 56 farmers and 16 veterinary assistants. Although there was no clear indication of underestimation of the weight of the animals and although farmers had adequate knowledge of the correct usage of isometamidium, the results suggest frequent underdosing when considering isometamidium serum concentrations 1 week after treatment. In 76% of the cases, the expected protection period was equal or shorter than 28 days and equal or shorter than 33 days in 90% of the treated cattle.


Subject(s)
Phenanthridines/therapeutic use , Trypanocidal Agents/therapeutic use , Trypanosoma , Trypanosomiasis, Bovine/prevention & control , Animals , Body Weight , Cattle , Drug Utilization , Enzyme-Linked Immunosorbent Assay/veterinary , Phenanthridines/blood , Surveys and Questionnaires , Trypanocidal Agents/blood , Trypanosomiasis, Bovine/parasitology , Zambia
20.
Acta Trop ; 84(1): 19-30, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12387907

ABSTRACT

Cattle from 50 farms in Mukono County, Uganda, were monitored for trypanosomes every second month over an 18-month period (1995-1996) by mini-anion exchange chromatography and haematocrit centrifugation techniques. Eighteen trypanosome isolates collected from cattle during this period were characterised in cattle, goats and mice for their sensitivity to homidium, isometamidium and diminazene; 10 of the isolates were selected randomly, 8 were from animals that had the highest serum isometamidium concentrations at the time the isolates were collected. All the isolates contained only Trypanosoma brucei and/or T. vivax. In nai;ve Boran (Bos indicus) cattle the isolates exhibited low pathogenicity and were sensitive to diminazene aceturate at 3.5 mg/kg body weight (bw) and isometamidium chloride at 0.5 mg/kg bw. In goats, 5 of 8 isolates were highly pathogenic, producing clinical signs indicative of central nervous system involvement within 60 days of infection; all such isolates contained T. brucei. However, all 8 populations were sensitive in goats to diminazene aceturate at 3.5 mg/kg bw. In contrast, 4 populations were refractory to treatment with isometamidium chloride at 0.5 mg/kg bw in at least 1 out of 3 goats each. Furthermore, 5 populations were refractory to treatment with homidium chloride at 1.0 mg/kg bw in a minimum of 2 out of 3 goats each. In mice, the 50% curative dose values for 11 Mukono isolates that contained T. brucei ranged from 0.30 to 1.89 mg/kg bw for diminazene aceturate, from 0.02 to 0.17 mg/kg bw for isometamidium chloride and from 0.90 to 4.57 mg/kg bw for homidium chloride. Thus, by comparison to reference drug-sensitive populations, all the stabilates were highly sensitive to diminazene and isometamidium, while some expressed low levels of resistance to homidium.


Subject(s)
Diminazene/analogs & derivatives , Trypanosoma brucei brucei/drug effects , Trypanosoma vivax/drug effects , Trypanosomiasis, African/prevention & control , Trypanosomiasis, Bovine/prevention & control , Animals , Cattle , Diminazene/pharmacology , Diminazene/therapeutic use , Disease Models, Animal , Drug Resistance , Ethidium/pharmacology , Female , Goat Diseases/prevention & control , Goats , Male , Mice , Phenanthridines/pharmacology , Phenanthridines/therapeutic use , Trypanocidal Agents/pharmacology , Trypanosoma brucei brucei/pathogenicity , Trypanosoma vivax/pathogenicity , Trypanosomiasis, African/veterinary , Uganda
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