Characterization of vaginal lactobacilli in women after kidney transplantation.

Limited number of publications described vaginal microflora after kidney transplantation. Our PubMed search revealed only 18 publications including words "vaginal bacteria & kidney transplant" in the period of 1978-2011. The aim of this study was to characterize lactobacilli isolated from vaginal swabs of women after kidney transplantation, compared with healthy women. Eighteen renal transplant recipients (mean age 36.1) and 20 healthy women (mean age 36.0) were evaluated. Lactobacilli were cultured on MRS and Columbia blood agars. Biochemical identification with API 50 CHL (bioMerieux, Marcy L'Etoile, France) and multiplex PCR according to Song et al. was performed. Lactobacilli were tested for production of H(2)O(2). Minimal inhibitory concentrations (MICs) of selected antimicrobial agents were determined with E-tests (bioMerieux, Marcy L'Etoile, France) and interpreted with CLSI and EUCAST criteria. No bacterial vaginosis was found among studied women. Two strains of group I were identified as Lactobacillus delbrueckii; 18 strains as Lactobacillus gasseri and 15 strains as Lactobacillus crispatus. Only 3 strains from group II were not identified by species-specific mPCR. Group IV was represented with 2 unidentified strains. Vaginal lactobacilli isolated from healthy women represented more homogenous group compared with heterogenous renal transplant recipients. Biochemical identification of lactobacilli by API 50 CHL kits was concordant with mPCR results only in 7 cases (17.5%), all 7 strains were identified as L. crispatus. Majority (93%) of lactobacilli were H(2)O(2) producers. All isolated lactobacilli (100%) demonstrated high resistance to metronidazole (MIC > 256 µg/ml). Only 2 strains resistant to vancomycin (MICs: 32 and 256 µg/ml respectively), in the study and control group, and one to moxifloxacin (MIC = 32 µg/ml), were found. Resistance to metronidazole and vancomycin was concordant in CLSI and EUCAST (2010) criteria. Although significant differences between lactobacilli isolated from vaginas of kidney transplant and healthy women were not demonstrated, we demonstrated strains resistant to metronidazole, vancomycin and moxifloxacin in groups of examined women. Our study was performed on a small group of kidney transplant recipients and further more detailed molecular studies on a larger group of patients are required to confirm our results.

[Analysis of occurrence of Chlamydophila pneumoniae infection serological markers in patients with bronchial asthma]. / Analiza wystepowania serologicznych markerów zakazenia Chlamydophila pneumoniae u chorych na astme oskrzelowa.

Numerous publications pointing the role of Chlamydophila pneumoniae infections in etiopathogenesis of bronchial asthma have appeared in recent years. Up to the presence the diagnostics of Chlamydophila pneumoniae infections has been based on the serological examinations especially on indirect microimmunofluorescence which is regarded to be a "gold standard". The aim of the paper was to compare the occurrence of Chlamydophila pneumoniae infection serological markers in the group of patients with asthma (N = 38) and in the control group (N = 38). The obtained results have allowed making the following conclusions: 1. The increased level of IgG class anti-Chlamydophila pneumoniae antibodies in the group of patients with asthma, which proves more frequent contact with Chlamydophila pneumoniae in that group, seems to support the hypothesis about the role of chlamydial infections in etiopathogenesis of bronchial asthma. 2. The single assessment of anti-Chlamydophila pneumoniae antibodies in the ELISA test has not allowed determining the type of chlamydial infection in unambiguous way. However, with reference to the entire clinical picture, the statement of the contact with Chlamydophila pneumoniae can be helpful especially in the treatment of severe asthma.

[Aerobic and anaerobic bacterial flora in chronic sinusitis in adults]. / Tlenowa i beztlenowa flora bakteryjna w przewleklym zapaleniu zatok przynosowych u doroslych.

The aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. Aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). Mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). The isolated aerobic bacteria were as follows: streptococci from the species Streptococcus salivarius, Streptococcus anginosus, Streptococcus group C, Streptococcus sanguis, Staphylococcus aureus, Gram-negative rods from the genus Haemophilus and rods from the Enterobacteriaceae family, and strains of Moraxella catarrhalis. The isolated anaerobic microorganisms Gram-negative rods from the genus Prevotella, Bacteroides, Fusobacterium, Gram-positive cocci from the genus Peptostreptococcus. Other organisms from the genus Vailonella, Eubacterium and Actinomyces were isolated less frequently. In 15 patients only one isolate was recovered, in 15 patients isolated bacteria were mixed with other microorganisms.

[Susceptibility to antibiotics of Staphylococcus aureus strains isolated from pus specimens obtained from patients referred for autovaccine therapy]. / Lekowrazliwosc szczepów Staphylococcus aureus izolowanych z ropy pacjentów kierowanych do leczenia autoszczepionka.

Autovaccine was prepared for 268 patients treated for: osteomyelitis--95, acne--63, furunculosis--53, and other pyogenic infections--57 patients. 540 bacterial strains were isolated from the specimens obtained from patients. 170 Staphylococcus aureus strains were cultured, what represents 31.5% of the isolated strains. Isolated Staphylococcus aureus strains were most often penicillin-resistant (91.5%), amplicillin-resistant (86%), less frequently tetracycline-resistant (73%), and doxycycline-resistant (53.1%). Staphylococcus aureus strains isolated from outpatients were found to be less resistant to particular antibiotics as compared to the group of strains isolated from the inpatients receiving treatment in different hospital departments. 13.5% of studied Staphylococcus aureus strains were methicillin-resistant.

[Etiologic bacterial factors of infections in patients treated at the clinical anesthesiology ward and with intensive therapy]. / Bakteryjne czynniki etiologiczne zakazen u chorych leczonych w Klinicznym Oddziale Anestezjologii i intensywnej Terapii.

Bacteriological investigations were carried on 264 patients treated in 1988-1990. 1736 samples of biological materials were taken and it amounts to 6-7 samples from each patient. Most frequently isolated microorganisms were: Pseudomonas aeruginosa (15%), Proteus mirabilis (13%), Klebsiella pneumoniae (10%) and Staphylococcus aureus (10%). Serological typing of Pseudomonas aeruginosa was performed according to Habs and domination of immunotype P16 (30%) was detected. Majority of isolated Klebsiella pneumoniae were not typable with basic and broadened phage set. One strain was susceptible to phages KI12 and KI27. This phage type was not isolated in Poland before. Staphylococci were most frequently susceptible to group II phages (29%), additional phages (19%) and 15% were not typable with the phage set used. Isolated bacteria were in majority resistant to numerous antibiotics.

[Bacteriologic examinations of patients with burns]. / Badania bakteriologiczne u chorych z ranami oparzeniowymi.

Bacteriological studies were performed on 189 patients treated during 1985-1989. 800 samples were tested which were taken from various sites of burn wound (3-5 smears from a patient). Surface of burn amounted to 15-86% and degree of burn was IIb/III. In 772 samples, 2073 bacterial strains were found. Most frequent were Pseudomonas aeruginosa (31%) and Staphylococcus aureus (29%). Remaining strains consisted chiefly of Proteus mirabilis, Pseudomonas cepacia, Klebsiella pneumoniae, Escherichia coli and Acinetobacter calcoaceticus. Serological typing of Pseudomonas aeruginosa carried according to Fisher demonstrated dominance of immunotype T3.7 (43%). Staphylococcal strains were most frequently sensitive to group III phages and/or supplementary phages.

[Effects of disodium salt of ethylenediaminetetraacetic acid (Na2EDTA) and tetracyclines on drug resistant bacteria. Studies in vitro]. / Badanie dzialania soli dwusodowej kwasu etylenodwuaminoczterooctowego (Na2EDTA) i tetracyklin na lekooporne bakterie. Badania in vitro.

An effect of Na2EDTA and tetracycline (oxytetracycline and doxycycline) resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa was tested. The strains were isolated from clinical specimens. The tests were performed in vitro by serial dilutions of the drugs in liquid medium. MIC for Na2EDTA, tetracyclines and a combination of Na2EDTA and tetracyclines was determined. It was shown that the combination of oxytetracycline or doxycycline with Na2EDTA caused changes in sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa resistant to these antibiotics. After an application of the mixture of various concentrations of tetracycline and Na2EDTA it was observed that, with the reduction of the effective Na2EDTA dose by about half, the lowest concentrations of tetracyclines inhibiting the growth of resistant bacteria were 2-64 times lower than MIC values of antibiotics without Na2EDTA.