ABSTRACT
We characterized the morphological and anatomical adaptations of the lingual microstructures of the Eurasian collared dove and discussed their implications for its dietary niche. We analyzed tongues of nine S. decaocto using histological, histochemical, stereomicroscopic, and scanning electron microscopic techniques. Our findings showed that the tongue is relatively short with a tapered apex that carries a terminal lingual nail. However, the lingual body has median scales and is bordered laterally by filiform papillae. Further, the tongue body bears a distinctive papillary crest. The tongue root is nonpapillate and infiltered with orifices of the posterior salivary glands. The bulky laryngeal mound has a circular glottic fissure, carrying a single row of papillae at the rear edge. Concurrently, our histological and histochemical findings demonstrate that the tongue has taste buds, anterior and posterior salivary glands, along with an elongated entoglossum that extends from lingual apex to root. Besides, ovoid and globular mucous glands displayed intense alcianophilic reactions. More substantially, the palate is made up of three palatine ridges with a caudal choanal cleft that was bounded by two rows of palatine papillae. Our data indicate multiple and novel structural variations for the lingual and palatal sculptures coopted for their feeding style.
ABSTRACT
We describe the functional morphology of the digestive tract of the Eurasian collared dove, Streptopelia decaocto using anatomical, morphometric, histological, histochemical, and ultrastructure techniques, and relate our findings to the species' dietary niche. Our results revealed that the esophagus is displaced on both sides of the neck and has highly folded tunica mucosa, which confer greater elasticity for efficient swallowing and passage of food to the crop. The proventriculus is delicate and its mucosal layer contains polymorphic glands with dense profound and superficial secretory units that open to the luminal surface by gastric pores. The ventriculus is biconvex and lined with a keratinized koilin membrane. The tubular glands within the mucosal lining include the isthmus, the neck, and the basal segment that comprise chief and basal cells with prominent nuclei. At the cuticle-mucosal interface, pyramidal vertical rodlets of the cuticle are secreted and superficially covered by a thin film of a horizontal matrix. The mucosa of the ileum form pyramidal villi that are oriented perpendicularly to the central lumen. Enterocytes infiltrated with goblet cells make up the epithelial lining of the villi. There are subtle differences in the thicknesses of corresponding tunics together with histochemical reactions of alcian blue (AB) and Masson-Goldner trichrome (MT) for their microstructures. Overall, our findings reveal remarkable convergence of both macro-and microstructures in S. decaocto to other granivorous species, and offer further evidence of the close association between functional morphology and feeding style relative to food swallowing, digestion, and absorption.
Subject(s)
Columbidae , Gastrointestinal Tract , Animals , Esophagus , Intestinal MucosaABSTRACT
The objective of our study was to explore the deleterious effects of diabetes on the visual functions of the retina and to address whether the administration of vitamin A and carrot root extract (CE) confer retinal protection in hyperglycemic rats via modulation of oxidative stress, biochemical alternations, and retinal neurotransmission. Fifty male Wistar albino rats weighing 180 ± 12.41 g were randomized into five groups (n = 10): controls, diabetic group (injected with 40 mg/kg dissolved in 0.1 sodium citrate buffer), diabetic group treated with vitamin A (2,500 IU/kg, low dose), diabetic group treated with vitamin (5,000 IU/kg, high dose), and diabetic groups administered CE (200 mg/kg/every other day). Our findings showed that, compared to controls, diabetic rats showed a significant decrease in their retinal thickness, increased apoptotic ganglion cells, and a noticeable degeneration of their synaptic layers. The inner retina displayed increased activity of neovascularization; however, the outer retina exhibited vacuolar degeneration of the photoreceptor cell layer. Our biochemical assessments showed reduced levels of CAT, SOD, and GST along with increased lipid peroxidation. Concurrently, cellular angiogenic and stress markers were significantly elevated associated with increased apoptotic activities as evidenced by increased expressions of annexin-V and PARP. Furthermore, the neurotransmitter content of the retina was altered in diabetic rats compared to controls and diabetic-treated groups. Paradoxically, vitamin A and CE supplementation attenuate these retinal insults in diabetic animals and normalized aforementioned assayed parameters; evidencing that both treatments exerted ameliorative impacts and restored visual functions by diminishing oxidative stress and neuronal degeneration. PRACTICAL APPLICATIONS: Diabetes is a complex disease that involves various physiological perturbations especially visual functions. In our study, we showed that vitamin A and carrot root extract (CE) confer remarkable protection against retinal degeneration in STZ-induced diabetic rats. Our findings showed that the chemical and phytochemical ingredients of the vitamin A and CE substantially attenuated the histopathological changes, oxidative stress, inflammatory reactions, and cellular death in diabetic rats. These favorable changes are attributable to the high content of retinoic acid, carotenoids, and phenolic compounds that effectively regulates the production of visual pigments, increases the antioxidant defense system, and diminishes the pro-inflammatory and apoptotic pathways. Thus, the nutritional values of vitamin A and CE represent promising therapeutic choices to mitigate the retinal-induced diabetic insults.
