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1.
Ultramicroscopy ; 103(4): 303-17, 2005 Jul.
Article in English | MEDLINE | ID: mdl-15885434

ABSTRACT

This paper presents an algorithm based on a continuous framework for a posteriori angular and translational assignment in three-dimensional electron microscopy (3DEM) of single particles. Our algorithm can be used advantageously to refine the assignment of standard quantized-parameter methods by registering the images to a reference 3D particle model. We achieve the registration by employing a gradient-based iterative minimization of a least-squares measure of dissimilarity between an image and a projection of the volume in the Fourier transform (FT) domain. We compute the FT of the projection using the central-slice theorem (CST). To compute the gradient accurately, we take advantage of a cubic B-spline model of the data in the frequency domain. To improve the robustness of the algorithm, we weight the cost function in the FT domain and apply a "mixed" strategy for the assignment based on the minimum value of the cost function at registration for several different initializations. We validate our algorithm in a fully controlled simulation environment. We show that the mixed strategy improves the assignment accuracy; on our data, the quality of the angular and translational assignment was better than 2 voxel (i.e., 6.54 angstroms). We also test the performance of our algorithm on real EM data. We conclude that our algorithm outperforms a standard projection-matching refinement in terms of both consistency of 3D reconstructions and speed.


Subject(s)
Microscopy, Electron/methods , Algorithms , Bacteriorhodopsins/chemistry , Bacteriorhodopsins/ultrastructure , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Microscopy, Electron/statistics & numerical data , Models, Molecular
2.
J Struct Biol ; 149(3): 243-55, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15721578

ABSTRACT

Measuring the quality of three-dimensional (3D) reconstructed biological macromolecules by transmission electron microscopy is still an open problem. In this article, we extend the applicability of the spectral signal-to-noise ratio (SSNR) to the evaluation of 3D volumes reconstructed with any reconstruction algorithm. The basis of the method is to measure the consistency between the data and a corresponding set of reprojections computed for the reconstructed 3D map. The idiosyncrasies of the reconstruction algorithm are taken explicitly into account by performing a noise-only reconstruction. This results in the definition of a 3D SSNR which provides an objective indicator of the quality of the 3D reconstruction. Furthermore, the information to build the SSNR can be used to produce a volumetric SSNR (VSSNR). Our method overcomes the need to divide the data set in two. It also provides a direct measure of the performance of the reconstruction algorithm itself; this latter information is typically not available with the standard resolution methods which are primarily focused on reproducibility alone.


Subject(s)
Image Processing, Computer-Assisted/methods , Imaging, Three-Dimensional , Microscopy, Electron , Algorithms , Mathematics , Models, Theoretical
3.
J Struct Biol ; 146(3): 381-92, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15099579

ABSTRACT

Three-dimensional (3D) electron microscopy (3DEM) aims at the determination of the spatial distribution of the Coulomb potential of macromolecular complexes. The 3D reconstruction of a macromolecule using single-particle techniques involves thousands of 2D projections. One of the key parameters required to perform such a 3D reconstruction is the orientation of each projection image as well as its in-plane orientation. This information is unknown experimentally and must be determined using image-processing techniques. We propose the use of wavelets to match the experimental projections with those obtained from a reference 3D model. The wavelet decomposition of the projection images provides a framework for a multiscale matching algorithm in which speed and robustness to noise are gained. Furthermore, this multiresolution approach is combined with a novel orientation selection strategy. Results obtained from computer simulations as well as experimental data encourage the use of this approach.


Subject(s)
Imaging, Three-Dimensional/methods , Macromolecular Substances/chemistry , Microscopy, Electron/methods , Algorithms , Bacteriorhodopsins/chemistry , Chaperonin 60/chemistry , Computer Simulation , Models, Molecular , Particle Size
4.
J Struct Biol ; 138(3): 216-26, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12217660

ABSTRACT

Beam damage is the main resolution-limiting factor when biological particles are observed by cryoelectron microscopy in a thin vitrified solution film. Furthermore, the low contrast of the specimen frequently makes observation difficult and limits the possibility of image processing. Cryo-negative staining, in which the particles are vitrified in a thin layer of concentrated ammonium molybdate solution, makes it possible to visualize the particles with a much better signal-to-noise ratio (SNR) while keeping the specimen in a good state of preservation. We have observed the Escherichia coli GroEL chaperonin, prepared in a native vitrified solution and by cryo-negative staining after electron exposure from 1000 to 3000e(-)/nm(2). We have compared the resulting three-dimensional models obtained from these different conditions and have tested their fit with the atomic model of the protein subunit obtained from X-ray crystallography. It is found that, down to 1.5-nm resolution, the particles appear to be faithfully represented in the cryo-negatively stained preparation, but there is an approximately 10-fold increase of SNR compared with the native vitrified preparation. Furthermore, for the same range of irradiation and down to the same resolution, the particles seem unaffected by beam damage, whereas the damage is severe in the native vitrified particles.


Subject(s)
Cryoelectron Microscopy/methods , Chaperonin 60/metabolism , Cryoelectron Microscopy/instrumentation , Crystallography, X-Ray , Electrons , Escherichia coli/ultrastructure , Image Processing, Computer-Assisted , Models, Molecular , Models, Statistical , Specimen Handling
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