Immunoelectron microscope localization of androgen receptors and proliferating cell nuclear antigen in the epithelial cells of albino rat ventral prostate.

Androgen receptor (AR) and proliferating cell nuclear antigen (PCNA) play a crucial role in development and progression of various prostatic diseases including prostatic carcinoma that is a leading cause of death in males. Previous studies have evaluated the expression pattern of AR and PCNA in prostate epithelial cells using immunohistochemistry (IHC). However, this technique has limited ability to identify their precise subcellular localization. Therefore, the aim of this study was to localize, subcellularly, AR and PCNA in the secretory epithelial cells of rat ventral prostate using post embedding immunogold-electron microscopy. The ventral lobes were dissected from six adult male albino rats after being perfused with paraformaldehyde. Some specimens were immuno-labeled with AR or PCNA and others were processed for immuno-electron microscope of AR and PCNA using 15-nm gold conjugated secondary antibodies. The results showed that, by immunoperoxidase reaction, AR and PCNA were localized diffusely throughout the nuclei of the epithelial cells of prostatic acini without visible cytoplasmic expression. However, the higher resolution immuno-electron microscopy was able to detect AR and PCNA in the nucleus and some cytoplasmic organelles. In conclusion, this study emphasizes the importance of immuno-electron microscopy in precise localization of AR and PCNA at the subcelullar levels in the secretory epithelial cells of the rat prostatic acini. These findings will help to further understand the mechanism of action of these receptors under normal and pathological conditions that could have future clinical application after careful human investigation.

Histological and immunohistochemical study of the effect of gold nanoparticles on the brain of adult male albino rat.

Gold nanoparticles (GNPs) have numerous medical applications as in biological imaging, cancer treatment and in implants (pacemakers and stents). Many conflicting results about GNPs safety and its accumulation in liver, kidney and brain were recorded. This work was carried out to study the histological effect of long period exposure to gold nanoparticle on the brain of adult male albino rat. Twenty adult male albino rats were divided into two equal groups: The first one served as a control group and the second one received 400 µg/kg/day GNPs by gastric tube once daily for eight weeks. Brain specimens were collected at the end of the experiment for histological and immunohistochemical studies using caspase-3 and glial fibrillary acidic protein (GFAP). GNPs treated group revealed wide spread histological alterations and deposition of gold nanoparticle aggregates in the neurons of cerebral cortex and hippocampus and also in the epithelium of choroid plexus with hyalinization of the wall of some blood vessels and disruption of the capillaries. All these changes were associated with localized positive caspase 3 reaction. Various degrees of astrogliosis were evidenced by astrocytic proliferation and increase size of their cell body with increase number and length of their processes. It could be concluded that repeated exposure of adult male albino rats to gold nanoparticles induced its deposition in the brain in association with histological alterations and various degrees of astrogliosis.