ABSTRACT
From Astragalus peregrinus, four cycloartane-type saponins have been isolated and their structures elucidated by spectral means as 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-beta-D-glucopyranoside (1), 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranoside (2), 20(R),24(S)-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,25-tetrol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside (3) and 20(R),25-epoxy-9 beta,19-cyclolanostane-3 beta,6 alpha,16 beta,24(S)-tetrol (24-O-acetyl)- 3-O-alpha-L-rhamnopyranosyl-(1-->2)-(6'-O-acetyl)-beta-D-glucopyranoside (4). Compounds 2 and 3 showed to stimulate the proliferation of mouse splenocytes and were not significantly cytotoxic.
Subject(s)
Adjuvants, Immunologic/pharmacology , Fabaceae , Lymphocytes/drug effects , Phytotherapy , Plant Extracts/pharmacology , Saponins/pharmacology , Adjuvants, Immunologic/therapeutic use , Animals , Female , Humans , Mice , Mice, Inbred Strains , Plant Extracts/therapeutic use , Plant Structures , Saponins/therapeutic use , Spleen/cytology , Spleen/drug effects , Tumor Cells, Cultured/drug effectsABSTRACT
Two new antimicrobial isoflavans, 1-[(3R)-7,8-dimethoxybenzopyranyl]-4- hydroxybenzoquinone (astragaluquinone) and (3S)-7,1'-dihydroxy-8,3'-dimethoxyisoflavan (8-methoxyvestitol), and the known 7-hydroxy-2',3',4'-trimethoxyisoflavan were isolated from roots of Astragalus alexandrinus and A. trigonus. The structures of the new isoflavans were established by spectroscopic methods.
Subject(s)
Anti-Infective Agents/pharmacology , Antifungal Agents/pharmacology , Benzopyrans/pharmacology , Isoflavones , Plants, Medicinal/chemistry , Quinones/pharmacology , Anti-Bacterial Agents , Anti-Infective Agents/chemistry , Antifungal Agents/chemistry , Benzopyrans/chemistry , Benzoquinones , Candida albicans/drug effects , Carbohydrate Sequence , Klebsiella pneumoniae/drug effects , Magnetic Resonance Spectroscopy , Medicine, Chinese Traditional , Microbial Sensitivity Tests , Micrococcus luteus/drug effects , Molecular Sequence Data , Plant Roots/chemistry , Quinones/chemistry , Saccharomyces cerevisiae/drug effects , Staphylococcus aureus/drug effectsABSTRACT
Four cycloartane triterpene oligoglycosides were isolated from the n-butanol extract of the aerial parts of Astragalus spinosus Vahl. (Leguminosae). They were identified as astragaloside I (1), isoastragaloside I (2), astragaloside IV (4) and cycloastragenol 6-O-glucoside (5) on the basis of comparing their m.p.'s, 1H NMR and 13C NMR spectra and chromatographic patterns with the data given in the literature. The results of AIDS antiviral and antitumor screening of the major component, astragaloside II (3), are dealt with.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Antiviral Agents/pharmacology , Glucosides/pharmacology , Plants, Medicinal/chemistry , CD4-Positive T-Lymphocytes/drug effects , CD4-Positive T-Lymphocytes/microbiology , Drug Screening Assays, Antitumor , Egypt , Glucosides/isolation & purification , HIV/drug effects , Humans , In Vitro Techniques , Magnetic Resonance SpectroscopyABSTRACT
A spectrophotometric method is presented for assaying Senna and its preparations, for both total sennosides and total rhein glycosides content. The method ensures complete elimination of other minor non-carboxylic anthracene derivatives, as well as flavonoidal contaminants. The proposed method quantitates the actual total sennosides content, through the elimination of these contaminants, and through correction for the interference due to the coexistence of rhein with sennidins, in the final determinative step. This would eliminate false high figures for total sennosides by earlier procedures and reflects, perspectively, the actual potency of the assayed samples.
Subject(s)
Anthraquinones/analysis , Glycosides/analysis , Senna Extract/analysis , Glucosides/analysis , Sennosides , Spectrophotometry/methods , Tablets/analysisABSTRACT
A new colorimetric method for glycyrrhizin in licorice and drug preparations is given. The method is based on coupling the acidic genin, glycyrrhetinic acid, with methylene blue and measuring the extinction of the coupled compound solution in chloroform-alcohol.
