Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Parasitol Res ; 115(11): 4251-4260, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27481172

ABSTRACT

Sixty specimens of African sharptooth catfish Clarias gariepinus (Clariidae) were collected during the period of March 2014-February 2015 from boat landing sites and fishermen of the River Nile at Beni Suef Governorate, Egypt, and examined for the presence of digenean parasites. The morphology of these parasites was studied by using light microscopy to describe morphological and morphometrically measurements for different body parts. Three digenean species belonging to three different genera were collected from various organs within the examined fish species. A certain degree of site specificity was also observed, with Thaparotrema botswanensis being found only in the gall bladder of C. gariepinus, while both Pseudoholorchis clarii and Glossidium pedatum were found only in the intestine and thus seem to occupy a certain niche within their host. This study represent as the first record of T. botswanensis and G. pedatum from C. gariepinus, as well as the first report of the genera from the River Nile in Egypt. In addition, re-description of P. clarii clarifies measurements for some body parts.


Subject(s)
Catfishes/parasitology , Fish Diseases/parasitology , Parasites/isolation & purification , Animals , Egypt , Geography , Intestines/parasitology , Microscopy , Parasites/genetics , Parasites/growth & development , Rivers/parasitology
2.
Int Immunopharmacol ; 24(2): 173-181, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25499729

ABSTRACT

Diethylcarbamazine citrate (DEC) had a significance in anti-filarial chemotherapy, while excretory-secretory product (ES) is released from adult filarial females. The target of the current study was to examine the immunomodulatory effect of DEC, Setaria equina ES or a combination of them on rat hepatocellular carcinoma (HCC) induced by diethylnitrosamine (DEN). In vitro effect of combined DEC and ES or ES alone on lipopolysaccharide (LPS)-stimulated rat peripheral blood mononuclear cells (PBMCs) was tested through IFN-γ assay in culture supernatants. In addition, single or repeated doses of DEC, ES or DEC+ES have been applied in white albino rats to test the effect on HCC. Levels of IFN-γ and anti-ES IgG antibodies in rat serum were assayed using ELISA. Hemolytic complement activity (CH50) was determined in serum while the concentration of nitric oxide (NO) was assayed in liver tissue. The infiltration of NK cells as well as the expression of MHC Iproliferating cell nuclear antigen (PCNA), inducible NO synthase (iNOS), Bcl2 and p53 were determined using immunohistochemistry. There was a dose-dependent increase in IFN-γ after in vitro exposure to DEC+ES. Repeated ES doses increased NO concentration (p<0.05) and expression of iNOS but reduced CH50 (p<0.001), while repeated DEC+ES doses could increase anti-ES IgG (p<0.01), IFN-γ level (p<0.05) and NK cell infiltration. The same treatments could also reduce the expression of MHC I expression, PCNA, Bcl2 and p53. This study has shown immunomodulatory and protective effects of DEC+ES repeated doses on rat HCC.


Subject(s)
Antigens, Helminth/administration & dosage , Carcinoma, Hepatocellular/drug therapy , Diethylcarbamazine/administration & dosage , Hepatocytes/drug effects , Killer Cells, Natural/drug effects , Leukocytes, Mononuclear/drug effects , Setaria Nematode/immunology , Animals , Carcinoma, Hepatocellular/chemically induced , Cells, Cultured , Diethylnitrosamine/administration & dosage , Female , Hepatocytes/pathology , Immunoglobulin G/blood , Immunomodulation , Interferon-gamma/metabolism , Killer Cells, Natural/immunology , Leukocytes, Mononuclear/immunology , Male , Nitric Oxide Synthase Type II/metabolism , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , Rats, Inbred Strains , Tumor Suppressor Protein p53/metabolism
3.
Arch Immunol Ther Exp (Warsz) ; 62(6): 511-20, 2014 Dec.
Article in English | MEDLINE | ID: mdl-24879096

ABSTRACT

Diethylcarbamazine citrate (DEC) has been known for its efficacy to eradicate bancroftian filariasis in Egypt and other countries in the world. One of the known effects was to decrease the level of circulating filarial antigen in the patient's serum. The target of this study was to examine the effect of DEC, excretory-secretory (ES) material from the filarial parasite Setaria equina or a combination of both on the status of oxidative stress and pathogenesis of rat hepatocellular carcinoma (HCC) induced by diethylnitrosamine and 2-acetylaminofluorene. This could be tested in vitro using nitroblue tetrazolium reduction test for measuring the level of superoxide anion (O2(•-)) released from rat peritoneal macrophages. For in vivo test, a single dose before induction of carcinogenesis or continually repeated doses with DEC, ES or DEC + ES was used. Exposure of macrophages to ES could lead to a significant decrease (p < 0.01) in O2(•-) release, while DEC (200 µM) could modulate such effect with significant increase (p < 0.05). Pathogenesis of liver cancer and treatment were evaluated using histological investigation, level of antioxidant and liver function enzymes. Repeated ES doses could increase the activity of antioxidant enzymes, especially the catalase enzyme and show a protective effect on liver architecture. DEC could modulate the later effects when combined with ES. No significant effect on the liver function enzymes after treatment was observed. Nuclear factor κB was found to be localized only in the cytoplasm after single and repeated treatments with ES. This study could indicate the effect of S. equina ES as antioxidant against rat HCC, while DEC could modulate such effect when combined with it.


Subject(s)
Diethylcarbamazine/therapeutic use , Liver Neoplasms, Experimental/drug therapy , Setaria Nematode , Alanine Transaminase/blood , Animals , Antioxidants/administration & dosage , Antioxidants/therapeutic use , Aspartate Aminotransferases/blood , Diethylcarbamazine/administration & dosage , Female , Lipid Peroxidation/drug effects , Liver/drug effects , Liver/metabolism , Liver/pathology , Liver Neoplasms, Experimental/metabolism , Liver Neoplasms, Experimental/pathology , NF-kappa B/metabolism , Oxidative Stress/drug effects , Rats , Setaria Nematode/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...