Bioprocess development for bacterial cellulose biosynthesis by novel Lactiplantibacillus plantarum isolate along with characterization and antimicrobial assessment of fabricated membrane.

Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. Its use is limited by the capacity of bacterial production strains and cost of the medium. Mining for novel organisms with well-optimized growth conditions will be important for the adoption of BC. In this study, a novel BC-producing strain was isolated from rotten fruit samples and identified as Lactiplantibacillus plantarum from 16S rRNA sequencing. Culture conditions were optimized for supporting maximal BC production using one variable at a time, Plackett-Burman design, and Box Behnken design approaches. Results indicated that a modified Yamanaka medium supported the highest BC yield (2.7 g/l), and that yeast extract, MgSO4, and pH were the most significant variables influencing BC production. After optimizing the levels of these variables through Box Behnken design, BC yield was increased to 4.51 g/l. The drug delivery capacity of the produced BC membrane was evaluated through fabrication with sodium alginate and gentamycin antibiotic at four different concentrations. All membranes (normal and fabricated) were characterized by scanning electron microscope, Fourier transform-infrared spectroscopy, X-ray diffraction, and mechanical properties. The antimicrobial activity of prepared composites was evaluated by using six human pathogens and revealed potent antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus mutans, with no detected activity against Pseudomonas aeruginosa and Candida albicans.

Application of either nano fibrillated cellulose methotrexate or nano silicon dioxide methotrexate composites against renal fibrosis in leukemia rat model.

Cellulose derivatives have got growing interest due to their relative abundance and ability to sustain the release of medicaments. In this study, micro- and nano-fibrillated cellulose were prepared from rice straw and used as drug carriers. Both carriers in addition to another one which is nano silicon dioxide were characterized with various techniques. Methotrexate was chosen to be loaded on nano-fibrillated cellulose and nano silicon dioxide. Both methotrexate carriers were evaluated for their possible protective role against renal fibrosis induced by methotrexate in leukemia rat model. Results of this study exhibited that loading methotrexate on either nano-fibrillated cellulose or nano silicon dioxide seems to have an ameliorative role on renal function tests, inflammatory and fibrotic markers of renal tissues. Moreover, the sustained release of methotrexate for long time period maintained by nano-fibrillated cellulose carrier gives it more priority than nano silicon dioxide to be used as an effective novel drug carrier in further medical applications with minimal side effects on kidney tissue in leukemia model.