The Effect of Different Routes of Injection of Bone Marrow Mesenchymal Stem Cells on Parotid Glands of Rats Receiving Cisplatin: A Comparative Study.

BACKGROUND AND OBJECTIVES: Cisplatin is a powerful antitumor chemotherapeutic agent that is widely used in the treatment of many cancers but it has many side effects on many organs including salivary glands. Bone marrow is considered to be a rich environment that comprises many types of stem cells of which BMSCs (Bone marrow mesenchymal stem cells) are the most studied with potentiality to differentiate into many cell types. This study was conducted to evaluate the effect of different routes of injection of BMSCs on parotid glands of rats receiving cisplatin. METHODS AND RESULTS: Sprague-Dawley rats were divided into 3 groups: a negative control group receiving phosphate buffered saline, a positive control group receiving cisplatin, and an experimental group where rats received cisplatin and then received iron oxide-labeled BMSCs by either intravenous or intraparotid routes or both. Animals were sacrificed at periods of 3,6,10 and 15 days after cisplatin injection, then histological, ultrastructural and immunohistochemical studies were done. The experimental stem cell treated group showed better histological features and increased PCNA proliferation index when compared to the control. The systemic and combination groups showed better results than the local group. Iron oxide-labeled cells were detected with Prussian blue stain. CONCLUSIONS: This study proved that BMSCs can improve cisplatin induced cytotoxicity in parotid glands. Systemic administration showed to have a better effect than local intraparotid administration and comparable effect to combined administration.

Short-term effects of Verbena officinalis Linn decoction on patients suffering from chronic generalized gingivitis: Double-blind randomized controlled multicenter clinical trial.

OBJECTIVE: To evaluate the clinical efficacy of Verbena officinalis Linn decoction for patients with chronic generalized gingivitis in a double-blind randomized controlled multicenter clinical trial. METHOD AND MATERIALS: The patients in the test group and the control group were instructed to brush and floss. Additionally, the patients in the test group were asked to rinse their mouths with a V officinalis L decoction. The primary clinical outcome was the Gingival Index (GI). The GI and Plaque Index (PI) were measured at baseline (day 0), day 14, and day 28. RESULTS: Two hundred and sixty patients participated (control group = 130, and test group = 130). The clinical features of both the test and control groups were improved progressively throughout the time durations of day 0, day 14, and day 28 represented by highly significant decreases in both GI and PI (P < .001). The Mann-Whitney test revealed significant differences between the control and test groups for GI and PI at the 14-day examination and the 28-day examination (P < .001). At the beginning of the clinical trial, nonsignificant clinical differences were found following the statistical analyses of both GI (P = .981) and PI (P = .920) between the test and control groups. CONCLUSIONS: The tested V officinalis L decoction demonstrated efficacy in reducing tested indices and thus has a promising ameliorative effect for treating patients with chronic generalized gingivitis. CLINICAL SIGNIFICANCE: V officinalis L decoction has good clinical results with no adverse effects.

Vasculitis of dental pulp associated with cryoglobulinemia in hepatitis C virus patients: case report.

INTRODUCTION: This report presents a case of impacted lower third molar extracted for surgical reasons in patient with uncontrolled hepatitis C. After decalcification, dental pulp vasculature and its tissue quality were investigated. METHODS: Serial sections of 4-µm thickness along the midline buccolingually for the demineralized specimen were obtained, mounted on a glass slide, stained with hematoxylin-eosin, covered, and viewed under the light microscope. RESULTS: The histologic investigation of the pulp tissue revealed thickening, stenosis, and occlusion of the vessel wall, ectopic calcification of the pulp tissue in close association with pulpal blood vessels, interrupted and vacuolated odontoblastic layer in the coronal pulp chamber, with an inflammatory cell infiltrate throughout the pulpal tissue. CONCLUSIONS: Cryoglobulinemia associated with uncontrolled hepatitis C virus infection in patients endangers the dental pulp vasculature and alters its normal tissue architecture.

Effect of 16% carbamide peroxide bleaching gel on enamel and dentin surface micromorphology and roughness of uremic patients: an atomic force microscopic study.

OBJECTIVES: To investigate the effect of 16% carbamide peroxide bleaching gel on surface micromorphology and roughness of enamel and root dentin of uremic patients receiving hemodialysis using atomic force microscopy (AFM). METHODS: A total of 20 sound molars were collected from healthy individuals (n=10) and uremic patients (n=10). The roots were separated from their crowns at the cemento-enamel junction. Dental slabs (3 mm x 2 mm x 2 mm) were obtained from the buccal surface for enamel slabs and the cervical third of the root surface for dentin slabs. Dental slabs were then flattened and serially polished up to #2500-grit roughness using silicon carbide abrasive papers. Half of the slabs obtained from healthy individuals and uremic patients were stored in artificial saliva and left without bleaching for control and comparison. The remaining half was subjected to a bleaching treatment using 16% carbamide peroxide gel (Polanight, SDI Limited) 8 h/day for 14 days and stored in artificial saliva until AFM analysis was performed. Statistical analysis of the roughness average (Ra) results was performed using one-way ANOVA and Bonferroni post hoc multiple comparisons test. RESULTS: The micromorphological observation of bleached, healthy enamel showed exaggerated prism irregularities more than non-bleached specimens, and this observation was less pronounced in bleached uremic enamel specimens with the lowest Ra. Bleached healthy dentin specimens showed protruded peritubular dentin and eroded intertubular dentin with the highest Ra compared to bleached uremic dentin. CONCLUSIONS: The negative effects of the bleaching gel on uremic tooth substrates are less dramatic and non-destructive compared to healthy substrates because uremia confers different micromorphological surface changes.

Predictive value of cyclooxygenase 2 and Bcl-2 for cervical lymph node metastasis in mucoepidermoid carcinoma.

The expression of cyclooxygenase 2 (COX-2) is induced by growth factors, tumor promoters, and cytokines. It is correlated with carcinogenesis and apoptosis inhibition. This study was designed to investigate the expression of COX-2 and BCl-2 and to correlate their expressions with the clinicopathologic features in the mucoepidermoid carcinoma (MEC). The expression of COX-2 and BCl-2 proteins was investigated in 16 archival tumor tissues of MEC using the streptavidin-biotin complex technique. Clinical information was obtained through the computerized retrospective database from the tumor registry between 2001 and 2007. It revealed that grading system of MEC did not correlate with the presence or absence of node metastasis. The expression of COX-2 and BCl-2 was variably expressed in all the examined specimens. COX-2 and BCl-2 immunoreactivity was observed mainly in the cytoplasm of neoplastic cells. As regard the clinicopathologic parameters, there was no significant difference in expression rates of COX-2 in patients among age, sex, and MEC grades (P > .05). However, the expression of COX-2 in node-positive tumors was significantly higher than that of node-negative tumors (P = .001). For BCL-2 expression, there was no significant difference in expression rates of BCl-2 in patients among age, sex, site, clinical stage, and lymph node metastasis (P > .05), whereas a high significant difference was observed between BCl-2 staining index and MEC grades (P = .027). Moreover, there is a positive correlation between COX-2 expression and BCL-2 staining index (P = .000). COX-2 is a good predictor for lymph node metastasis as well as ideal therapeutic target for the prevention or treatment of MEC. BCl-2 and COX-2 are potentially useful prognostic markers for MEC.