A Novel Hybrid Approach for Classifying Osteosarcoma Using Deep Feature Extraction and Multilayer Perceptron.

Osteosarcoma is the most common type of bone cancer that tends to occur in teenagers and young adults. Due to crowded context, inter-class similarity, inter-class variation, and noise in H&E-stained (hematoxylin and eosin stain) histology tissue, pathologists frequently face difficulty in osteosarcoma tumor classification. In this paper, we introduced a hybrid framework for improving the efficiency of three types of osteosarcoma tumor (nontumor, necrosis, and viable tumor) classification by merging different types of CNN-based architectures with a multilayer perceptron (MLP) algorithm on the WSI (whole slide images) dataset. We performed various kinds of preprocessing on the WSI images. Then, five pre-trained CNN models were trained with multiple parameter settings to extract insightful features via transfer learning, where convolution combined with pooling was utilized as a feature extractor. For feature selection, a decision tree-based RFE was designed to recursively eliminate less significant features to improve the model generalization performance for accurate prediction. Here, a decision tree was used as an estimator to select the different features. Finally, a modified MLP classifier was employed to classify binary and multiclass types of osteosarcoma under the five-fold CV to assess the robustness of our proposed hybrid model. Moreover, the feature selection criteria were analyzed to select the optimal one based on their execution time and accuracy. The proposed model achieved an accuracy of 95.2% for multiclass classification and 99.4% for binary classification. Experimental findings indicate that our proposed model significantly outperforms existing methods; therefore, this model could be applicable to support doctors in osteosarcoma diagnosis in clinics. In addition, our proposed model is integrated into a web application using the FastAPI web framework to provide a real-time prediction.

A patient-specific functional module and path identification technique from RNA-seq data.

With the advancement of new technologies, a huge amount of high dimensional data is being generated which is opening new opportunities and challenges to the study of cancer and diseases. In particular, distinguishing the patient-specific key components and modules which drive tumorigenesis is necessary to analyze. A complex disease generally does not initiate from the dysregulation of a single component but it is the result of the dysfunction of a group of components and networks which differs from patient to patient. However, a patient-specific network is required to understand the disease and its molecular mechanism. We address this requirement by constructing a patient-specific network by sample-specific network theory with integrating cancer-specific differentially expressed genes and elite genes. By elucidating patient-specific networks, it can identify the regulatory modules, driver genes as well as personalized disease networks which can lead to personalized drug design. This method can provide insight into how genes are associating with each other and characterized the patient-specific disease subtypes. The results show that this method can be beneficial for the detection of patient-specific differential modules and interaction between genes. Extensive analysis using existing literature, gene enrichment and survival analysis for three cancer types STAD, PAAD and LUAD shows the effectiveness of this method over other existing methods. In addition, this method can be useful for personalized therapeutics and drug design. This methodology is implemented in the R language and is available at https://github.com/riasatazim/PatientSpecificRNANetwork.

An Explainable AI Approach for the Rapid Diagnosis of COVID-19 Using Ensemble Learning Algorithms.

Background: Artificial intelligence-based disease prediction models have a greater potential to screen COVID-19 patients than conventional methods. However, their application has been restricted because of their underlying black-box nature. Objective: To addressed this issue, an explainable artificial intelligence (XAI) approach was developed to screen patients for COVID-19. Methods: A retrospective study consisting of 1,737 participants (759 COVID-19 patients and 978 controls) admitted to San Raphael Hospital (OSR) from February to May 2020 was used to construct a diagnosis model. Finally, 32 key blood test indices from 1,374 participants were used for screening patients for COVID-19. Four ensemble learning algorithms were used: random forest (RF), adaptive boosting (AdaBoost), gradient boosting decision tree (GBDT), and extreme gradient boosting (XGBoost). Feature importance from the perspective of the clinical domain and visualized interpretations were illustrated by using local interpretable model-agnostic explanations (LIME) plots. Results: The GBDT model [area under the curve (AUC): 86.4%; 95% confidence interval (CI) 0.821-0.907] outperformed the RF model (AUC: 85.7%; 95% CI 0.813-0.902), AdaBoost model (AUC: 85.4%; 95% CI 0.810-0.899), and XGBoost model (AUC: 84.9%; 95% CI 0.803-0.894) in distinguishing patients with COVID-19 from those without. The cumulative feature importance of lactate dehydrogenase, white blood cells, and eosinophil counts was 0.145, 0.130, and 0.128, respectively. Conclusions: Ensemble machining learning (ML) approaches, mainly GBDT and LIME plots, are efficient for screening patients with COVID-19 and might serve as a potential tool in the auxiliary diagnosis of COVID-19. Patients with higher WBC count, higher LDH level, or higher EOT count, were more likely to have COVID-19.