Fe3O4@PDA@PANI core-shell nanocomposites as a new adsorbent for simultaneous preconcentration of Tartrazine and Sunset Yellow by ultrasonic-assisted dispersive micro solid-phase extraction.

In this research, novel magnetic Fe3O4@PDA@PANI core-shell nanoparticles were designed and fabricated as an efficient adsorbent in the service of ultrasound-assisted dispersive micro-solid phase extraction for simultaneous preconcentration of Sunset Yellow (SY) and Tartrazine (Tar) before UV-Vis spectrophotometric detection. This adsorbent was fully characterized by Fourier Transform Infrared (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy Dispersive X-ray (EDX) analysis. To overcome the spectral overlapping of SY and Tar dyes, the derivative spectrophotometric method was successfully used for the simultaneous detection of dyes in their binary solutions. The operating parameters affecting preconcentration efficiency and spectrophotometric determination were optimized. Under optimal conditions, the limit of detections (LOD) was obtained 0.2 and 0.5 ng mL-1 for SY and Tar, respectively. The adsorption capacity and reusability of core-shell nanoparticles were significant. The satisfactory results of analysis of a few real samples indicate that the method is very favored in the analysis of various complex matrices.

Colorimetric detection of insulin in human serum using GO/AuNPs/TX-100 nanocomposite.

In this study, graphene oxide/gold nanoparticles/Triton X-100 nanocomposites (GO/AuNPs/TX-100) were synthesized using the sonochemical method and their ability in ultrasound-assisted colorimetric detection of insulin was investigated. The synthesized GO/AuNPs/TX-100 nanocomposites were characterized by UV-visible absorption spectroscopy and TEM analysis. The interaction between the nanocomposites and insulin was observed by both naked eye and optical absorption spectroscopy. The GO/AuNPs/TX-100 nanocomposites displayed apparent color changes (red to blue) and absorption spectra changes (decreasing of the band around 528 nm and appearance of a new red-shifted band at 640 nm) in presence of insulin. The interaction mechanism of the nanocomposites and insulin was discussed. It is based on the special structure of insulin, that insulin can be easily self-assemble into the GO/AuNP/TX-100 nanocomposites and can also play the role of a bridge between two different GO/AuNPs/TX-100 nanocomposites by peptide chains. The effective parameters for insulin detection were optimized. The colorimetric method was used for quantification of insulin in the range of 2-300 ng mL-1 with a detection limit of 0.1 ng mL-1. Moreover, the relative standard deviation of the method was 3.1 and 2.7% (n = 10) at concentrations of 50 and 200 ng mL-1, respectively on the same day and 4.8% at a concentration (200.0 ng mL-1) on five consecutive days. The present method was utilized for insulin assay in human blood serums with satisfactory results.

Sensitive, simple and rapid colorimetric detection of malachite green in water, salmon and canned tuna samples based on gold nanoparticles.

BACKGROUND: Malachite green is used in aquaculture and fisheries as a fungicide and antiseptic and it is also used in industry as a dye. However, malachite green is carcinogenic and highly toxic for humans and animals. In this study, a spectrophotometric method was developed to detect malachite green. The method was based on the surface plasmon resonance property of gold nanoparticles and interaction between malachite green and gold nanoparticles. RESULTS: Malachite green-gold nanoparticles were rapidly aggregated in the acidic medium; as a result, a color change from red to blue was observed, which was easily detectable by the naked eye. The absorption ratio (A623/A520) of the gold nanoparticles in an optimized system exhibited a linear correlation with malachite green concentration. The method detection limit and linear range were 3 and 50-350 ng mL-1 , respectively. The method was applied successfully to detect malachite green in different samples. CONCLUSION: The method was simple and rapid to detect malachite green. The most important advantages of the method are the possibility of malachite green determination with very good accuracy and sensitivity using a simple UV-visible spectrometer without any expensive or sophisticated instrumentation and also the versatility of real samples. © 2018 Society of Chemical Industry.

Surface plasmon resonance of gold nanoparticles as a colorimetric sensor for indirect detection of Cefixime.

This study reports a colorimetric sensor with excellent sensitivity to detect Cefixime base on gold nanoparticles. Cefixime is an antibiotic which has a wide range of applications in medicine. Cefixime did not change the surface plasmon resonance bond in gold nanoparticles solution; therefore, there was no change in the color solution of gold nanoparticles. The presence of Alizarin Red S in the system was necessary for the degradation of Cefixime, resulting in the aggregation of gold nanoparticles and a color change from red to blue. As a result of aggregation, the localized surface plasmon resonance band of gold nanoparticles decreased to around 525 nm and a new red-shifted band at 640 nm appeared which increases gradually as the function of Cefixime concentration. A unique detection limit (2.5 ng mL-1) was achieved for Cefixime in comparison with other colorimetric methods. Relative standard deviations (RSD) for 40.0 and 140.0 ng mL-1 of Cefixime were 2.6 and 1.8% for intra-day respectively. A possible mechanism was discussed for the surface plasmon resonance changes of AuNPs in the presence of Cefixime. The proposed method was applied to detect Cefixime in pharmaceutical samples with satisfactory results. This system is low-cost and is highly sensitive with no need for any preconcentration steps or using any expensive or sophisticated instrumentation.

Highly sensitive detection of Ceftriaxone in water, food, pharmaceutical and biological samples based on gold nanoparticles in aqueous and micellar media.

A colorimetric assay with excellent sensitivity is reported to detect Ceftriaxone in aqueous and micellar solutions. Ceftriaxone could induce the aggregation of gold nanoparticles through hydrogen-bonding interaction and electrostatic attraction. As a result of aggregation, the surface plasmon resonance band around 520 nm decreases and a new band appears at 620 nm. The effect of surfactants was investigated on the aggregation. The band around 620 nm is shifted to around 685 nm in Triton X-100 micellar media and that is seen color conversion from red to deep blue which is clearly detectable by the naked eye. The results were improved in Triton X-100 micellar media as compared to aqueous media so that the lowest measured concentration and detection limit in micellar media have decreased 10 and 8 times, respectively. Triton X-100 showed strong effect on the stabilization of the solutions. The method has been successfully applied for the analysis of various real samples.

Development of a Dispersive Liquid-Liquid Microextraction Method Combined with UV-Visible Spectrophotometry for Determination of Trace Aluminum(III) in Water, Wastewater, Food, Biological, and Pharmaceutical Samples.

A simple and sensitive method was proposed for the preconcentration of trace levels of Al(III) prior to its determination by spectrophotometry, based on dispersive liquid-liquid microextraction. The complexation of the Al(III) was performed by chelation with Eriochrome Cyanine R (ECR). In this method, cetyltrimethyl ammonium bromide (CTAB) as a dispersant was dissolved in chloroform as an extractant solvent, and then the solution was rapidly injected by a syringe into the samples containing Al(III), which had already been complexed by ECR at optimized pH. Various parameters were studied and optimized for a 10 mL sample volume. Under the optimum conditions, the LOD (3 times the SD of 10 replicate readings of the reagent blank) and the dynamic range of the calibration obtained were 0.2 ng mL-1 (7 nM) and 1.0-80.0 ng mL-1, respectively. The RSDs for eight replicate determinations of 10 and 60 ng mL-1 of Al(III) were 3.3 and 1.8%, respectively. This strategy was successfully applied to determine the Al concentration in water, wastewater, yogurt, apple, carrot, celery, bread, potato, urine, and Al-Mg syrup samples.