Enzyme-Mediated Nerve Growth Factor Release from Nanofibers Using Gelatin Microspheres.

Spinal cord injury is a complex environment, with many conflicting growth factors present at different times throughout the injury timeline. Delivery of multiple growth factors has received mixed results, highlighting a need to consider the timing of delivery for possibly antagonistic growth factors. Cell-mediated degradation of delivery vehicles for delayed release of growth factors offers an attractive way to exploit the highly active immune response in the spinal cord injury environment. In this study, growth factor-loaded gelatin microspheres (GMS) combined with methacrylated hyaluronic acid (MeHA) were electrospun to create GMS fibers (GMSF) for delayed release of growth factors (GFs). GMS were successfully combined with MeHA while electrospinning, with an average fiber diameter of 365 ± 10 nm and 44% ± 8% fiber alignment. GMSF with nerve growth factor (NGF) was tested on dissociated chick dorsal root ganglia cells. We further tested the effect of M1 macrophage-conditioned media (M1CM) to simulate macrophage invasion after spinal cord injury for cell-mediated degradation. We hypothesized that neurons grown on GMSF with loaded NGF would exhibit longer neurites in M1CM, showing a release of functional NGF, as compared with controls. GMSF in M1CM was significantly different from MeHA in serum-free media (SFM) and M0-conditioned media (M0CM), as well as GMSF in M0CM (p < 0.05). Moreover, GMSF + NGF in all media conditions were significantly different from MeHA in SFM and M0CM (p < 0.05). The goal of this study was to develop a biomaterial system where drug delivery is triggered by immune response, allowing for more control and longer exposure to encapsulated drugs. The spinal cord injury microenvironment is known to have a robust immune response, making this immune-medicated drug release system particularly significant for directed repair.

Plantar loading characteristics during walking in females with and without patellofemoral pain.

BACKGROUND: Patellofemoral pain (PFP) is a common injury, particularly in females. Foot pronation may promote knee and hip transverse plane joint kinematics during gait thought to contribute to PFP. Greater knowledge of plantar loading characteristics in females with PFP may be valuable to provide a basis for clinical decisions regarding footwear and foot orthoses. The purpose of this study was to compare plantar loading distribution in females with and without PFP during gait. METHODS: Plantar pressure during walking was recorded from 19 females with PFP and 20 females without PFP. Contact area, peak force, and force-time integral were evaluated in ten plantar areas. Arch index was also calculated from contact area data during gait. RESULTS: Contact area in females with PFP was 9% smaller in the first metatarsal region (P = .039) and 20% smaller in the midfoot region (P = .042) than in females without PFP. Peak force was 31% lower in the midfoot region for females with PFP (P = .027) and 13% lower in the first metatarsal region (P = .064). Force-time integral was 18% lower in the first metatarsal region in females with PFP (P = .024). Females with PFP demonstrated a lower arch index (suggesting a higher arch) (P = .028). CONCLUSIONS: Decreased medial forefoot loading and decreased midfoot contact suggest decreased foot pronation during gait in females with PFP relative to females without PFP. Decreased foot pronation may foster increased patellofemoral joint loading rates. These data contribute to rationale for footwear modifications to modify plantar loading characteristics in people experiencing PFP.

Patient receptivity to tobacco cessation counseling in an academic dental clinic: a patient survey.

OBJECTIVE: This study investigated dental patient attitudes towards tobacco cessation counseling in the dental school setting. METHODS: Patients attending an academic dental clinic during a six-week period were asked to complete a 22-item self-administered survey. Means and frequency distributions were assessed on all variables as appropriate. RESULTS: Response rate was 71%. Twenty-nine percent of respondents reported that they currently used tobacco. Seventy-two percent of tobacco users agreed that the student dentist should ask patients whether or not they use tobacco, 67% agreed that the student dentist should advise tobacco users to quit, and 89% agreed that student dentists should offer quit tobacco information to patients who want to quit. Seventy percent of tobacco users were considering quitting or currently trying to quit. Of these, only 31% were aware of community resources to help them quit. CONCLUSIONS: The majority of tobacco users were positive in their attitudes toward delivery of tobacco cessation counseling and services in the dental setting. Many were considering or trying to quit, but few were aware of community resources to help them.