Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters










Publication year range
1.
J Immunol ; 208(1): 38-48, 2022 01 01.
Article in English | MEDLINE | ID: mdl-34862257

ABSTRACT

RNA-binding protein HuR (ELAVL1) is a master regulator of gene expression in human pathophysiology. Its dysregulation plays an important role in many diseases. We hypothesized that HuR plays an important role in Th2 inflammation in asthma in both mouse and human. To address this, we used a model of airway inflammation in a T cell-specific knockout mouse model, distal lck-Cre HuRfl/fl, as well as small molecule inhibitors in human peripheral blood-derived CD4+ T cells. Peripheral CD4+ T cells were isolated from 26 healthy control subjects and 45 asthmatics (36 type 2 high and 9 non-type 2 high, determined by blood eosinophil levels and fraction of exhaled NO). Our mouse data showed conditional ablation of HuR in T cell-abrogated Th2 differentiation, cytokine production, and lung inflammation. Studies using human T cells showed that HuR protein levels in CD4+ T cells were significantly higher in asthmatics compared with healthy control subjects. The expression and secretion of Th2 cytokines were significantly higher in asthmatics compared with control subjects. AMP-activated protein kinase activator treatment reduced the expression of several cytokines in both type 2 high and non-type 2 high asthma groups. However, the effects of CMLD-2 (a HuR-specific inhibitor) were more specific to endotype-defining cytokines in type 2 high asthmatics. Taken together, these data suggest that HuR plays a permissive role in both allergen and non-allergen-driven airway inflammation by regulating key genes, and that interfering with its function may be a novel method of asthma treatment.


Subject(s)
Asthma/metabolism , ELAV-Like Protein 1/metabolism , Th2 Cells/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Allergens/immunology , Animals , Anti-Inflammatory Agents/pharmacology , Asthma/genetics , Asthma/therapy , Benzopyrans/pharmacology , Cells, Cultured , Disease Models, Animal , ELAV-Like Protein 1/antagonists & inhibitors , ELAV-Like Protein 1/genetics , Humans , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Middle Aged , Ovalbumin/immunology , Pyrrolidines/pharmacology , Young Adult
2.
J Immunol ; 199(7): 2236-2248, 2017 10 01.
Article in English | MEDLINE | ID: mdl-28801358

ABSTRACT

IL-4 and IL-13 have been defined as anti-inflammatory cytokines that can counter myelin-reactive T cells and modulate experimental allergic encephalomyelitis. However, it is not known whether endogenous IL-4 and IL-13 contribute to the maintenance of peripheral tolerance and whether their function is coordinated with T regulatory cells (Tregs). In this study, we used mice in which the common cytokine receptor for IL-4 and IL-13, namely the IL-4Rα/IL-13Rα1 (13R) heteroreceptor (HR), is compromised and determined whether the lack of signaling by endogenous IL-4 and IL-13 through the HR influences the function of effector Th1 and Th17 cells in a Treg-dependent fashion. The findings indicate that mice-deficient for the HR (13R-/-) are more susceptible to experimental allergic encephalomyelitis than mice sufficient for the HR (13R+/+) and develop early onset and more severe disease. Moreover, Th17 cells from 13R-/- mice had reduced ability to convert to Th1 cells and displayed reduced sensitivity to suppression by Tregs relative to Th17 effectors from 13R+/+ mice. These observations suggest that IL-4 and IL-13 likely operate through the HR and influence Th17 cells to convert to Th1 cells and to acquire increased sensitivity to suppression, leading to control of immune-mediated CNS inflammation. These previously unrecognized findings shed light on the intricacies underlying the contribution of cytokines to peripheral tolerance and control of autoimmunity.


Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Interleukin-13 Receptor alpha1 Subunit/immunology , Receptors, Cell Surface/immunology , T-Lymphocytes, Regulatory/immunology , Th17 Cells/immunology , Animals , Cytokines/biosynthesis , Cytokines/immunology , Disease Models, Animal , Immune Tolerance , Interleukin-13/biosynthesis , Interleukin-13/metabolism , Interleukin-13 Receptor alpha1 Subunit/deficiency , Interleukin-13 Receptor alpha1 Subunit/genetics , Interleukin-4/biosynthesis , Interleukin-4/metabolism , Mice , Mice, Inbred C57BL , Receptors, Cell Surface/deficiency , Receptors, Cell Surface/genetics , Signal Transduction , Th1 Cells/immunology
3.
J Clin Med ; 6(2)2017 Jan 26.
Article in English | MEDLINE | ID: mdl-28134752

ABSTRACT

Experiments with B cell-deficient (B-/-) mice indicate that a number of autoimmune diseases require B cells in addition to T cells for their development. Using B-/- Non-obese diabetic (NOD) and NOD.H-2h4 mice, we demonstrated that development of spontaneous autoimmune thyroiditis (SAT), Sjogren's syndrome and diabetes do not develop in B-/- mice, whereas all three diseases develop in B cell-positive wild-type (WT) mice. B cells are required early in life, since reconstitution of adult mice with B cells or autoantibodies did not restore their ability to develop disease. B cells function as important antigen presenting cells (APC) to initiate activation of autoreactive CD4+ effector T cells. If B cells are absent or greatly reduced in number, other APC will present the antigen, such that Treg are preferentially activated and effector T cells are not activated. In these situations, B-/- or B cell-depleted mice develop the autoimmune disease when T regulatory cells (Treg) are transiently depleted. This review focuses on how B cells influence Treg activation and function, and briefly considers factors that influence the effectiveness of B cell depletion for treatment of autoimmune diseases.

4.
Immunohorizons ; 1(6): 109-123, 2017 Aug 01.
Article in English | MEDLINE | ID: mdl-30035254

ABSTRACT

Posttranscriptional gene regulation by RNA-binding proteins, such as HuR (elavl1), fine-tune gene expression in T cells, leading to powerful effects on immune responses. HuR can stabilize target mRNAs and/or promote translation by interacting with their 3' untranslated region adenylate and uridylate-rich elements. It was previously demonstrated that HuR facilitates Th2 cytokine expression by mRNA stabilization. However, its effects upon IL-2 homeostasis and CD4+ Th2 differentiation are not as well understood. We found that optimal translation of Il2ra (CD25) required interaction of its mRNA with HuR. Conditional HuR knockout in CD4+ T cells resulted in loss of IL-2 homeostasis and defects in JAK-STAT signaling, Th2 differentiation, and cytokine production. HuR-knockout CD4+ T cells from OVA-immunized mice also failed to proliferate in response to Ag. These results demonstrate that HuR plays a pivotal role in maintaining normal IL-2 homeostasis and initiating CD4+ Th2 differentiation.

5.
Immunology ; 147(4): 464-75, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26749165

ABSTRACT

Effective humoral immunity ensues when antigen presentation by B cells culminates in productive cooperation with T lymphocytes. This collaboration, however, remains ill-defined because naive antigen-specific B cells are rare and difficult to track in vivo. Herein, we used a defined transfer model to examine how B lymphocytes, as antigen-presenting cells, shape the development of T-cell memory suitable for generation of relevant antibody responses. Specifically, we examined how B cells presenting different doses of antigen during the initial priming phase shape the development of CD4 T-cell memory and its influence on humoral immunity. The findings indicate that B cells presenting low dose of antigen favour the development of T helper type 1 (Th1) type memory, while those presenting a high antigen dose yielded better Th2 memory cells. The memory Th2 cells supported the production of antibodies by effector B cells and promoted isotype switching to IgG1. Moreover, among the B-cell subsets tested for induction of Th2 memory, the splenic but not peritoneal B220(lo) cells were most effective in sustaining Th2 memory development as well as immunoglobulin isotype switching, and this function involved a tight control by programmed death 1-programmed death ligand 2 interactions.


Subject(s)
Antigen Presentation , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , Cell Communication/immunology , Immunoglobulin Class Switching , Immunologic Memory , Th2 Cells/immunology , Th2 Cells/metabolism , Animals , Antigen-Presenting Cells/immunology , Antigen-Presenting Cells/metabolism , Antigens/immunology , B-Lymphocyte Subsets/immunology , B-Lymphocyte Subsets/metabolism , Immunoglobulin G/genetics , Immunoglobulin G/immunology , Interleukin-5/biosynthesis , Leukocyte Common Antigens/metabolism , Mice , Mice, Knockout , Mice, Transgenic , Programmed Cell Death 1 Ligand 2 Protein/metabolism , Programmed Cell Death 1 Receptor/metabolism , Protein Binding , Spleen/immunology
6.
Immunology ; 144(4): 598-610, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25318356

ABSTRACT

NOD.H-2h4 mice develop spontaneous autoimmune thyroiditis (SAT) with chronic inflammation of thyroids by T and B cells. B-cell deficient (B(-/-) ) mice are resistant to SAT but develop SAT if regulatory T (Treg) cells are transiently depleted. We established a transfer model using splenocytes from CD28(-/-)  B(-/-) mice (effector cells and antigen-presenting cells) cultured with or without sorted Treg cells from Foxp3-GFP wild-type (WT) or B(-/-) mice. After transfer to mice lacking T cells, mice given Treg cells from B(-/-) mice had significantly lower SAT severity scores than mice given Treg cells from WT mice, indicating that Treg cells in B(-/-) mice are more effective suppressors of SAT than Treg cells in WT mice. Treg cells from B(-/-) mice differ from WT Treg cells in expression of CD27, tumour necrosis factor receptor (TNFR) II p75, and glucocorticoid-induced TNFR-related protein (GITR). After transient depletion using anti-CD25 or diphtheria toxin, the repopulating Treg cells in B(-/-) mice lack suppressor function, and expression of CD27, GITR and p75 is like that of WT Treg cells. If B(-/-) Treg cells develop with B cells in bone marrow chimeras, their phenotype is like that of WT Treg cells. Addition of B cells to cultures of B(-/-) Treg and T effector cells abrogates their suppressive function and their phenotype is like that of WT Treg cells. These results establish for the first time that Treg cells in WT and B(-/-) mice differ both functionally and in expression of particular cell surface markers. Both properties are altered after transient depletion and repopulation of B(-/-) Treg cells, and by the presence of B cells during Treg cell development or during interaction with effector T cells.


Subject(s)
B-Lymphocytes/immunology , T-Lymphocytes, Regulatory/immunology , Thyroiditis, Autoimmune/immunology , Adoptive Transfer , Animals , B-Lymphocytes/metabolism , Biomarkers/metabolism , Bone Marrow Transplantation , CD28 Antigens/deficiency , CD28 Antigens/genetics , Cell Communication , Cells, Cultured , Coculture Techniques , Disease Models, Animal , Genes, T-Cell Receptor alpha , Genetic Predisposition to Disease , Leukocyte Common Antigens/genetics , Mice, Inbred NOD , Mice, Knockout , Phenotype , Severity of Illness Index , T-Lymphocytes, Regulatory/metabolism , T-Lymphocytes, Regulatory/transplantation , Thyroiditis, Autoimmune/genetics , Thyroiditis, Autoimmune/metabolism , Thyroiditis, Autoimmune/prevention & control , Transplantation Chimera
7.
J Immunol ; 191(10): 4940-9, 2013 Nov 15.
Article in English | MEDLINE | ID: mdl-24098053

ABSTRACT

NOD.H-2h4 mice given NaI in their drinking water develop iodine-accelerated spontaneous autoimmune thyroiditis (ISAT) with chronic inflammation of the thyroid by T and B cells and production of anti-mouse thyroglobulin (MTg) autoantibody. CD28(-/-) NOD.H-2h4 mice, which have reduced numbers of CD4(+)Foxp3(+) regulatory T cells (Tregs), were developed to examine the role of Tregs in ISAT development. CD28(-/-) NOD.H2-h4 mice develop more severe ISAT than do wild-type (WT) mice, with collagen deposition (fibrosis) and low serum T4. CD28(-/-) mice have increased expression of proinflammatory cytokines IFN-γ and IL-6, consistent with increased mononuclear cell infiltration and tissue destruction in thyroids. Importantly, transferring purified CD4(+)Foxp3(+) Tregs from WT mice reduces ISAT severity in CD28(-/-) mice without increasing the total number of Tregs, suggesting that endogenous Tregs in CD28(-/-) mice are functionally ineffective. Endogenous CD28(-/-) Tregs have reduced surface expression of CD27, TNFR2 p75, and glucocorticoid-induced TNFR-related protein compared with transferred CD28(+/+) Tregs. Although anti-MTg autoantibody levels generally correlate with ISAT severity scores in WT mice, CD28(-/-) mice have lower anti-MTg autoantibody responses than do WT mice. The percentages of follicular B cells are decreased and those of marginal zone B cells are increased in spleens of CD28(-/-) mice, and they have fewer thyroid-infiltrating B cells than do WT mice. This suggests that CD28 deficiency has direct and indirect effects on the B cell compartment. B cell-deficient (B(-/-)) NOD.H-2h4 mice are resistant to ISAT, but CD28(-/-)B(-/-) mice develop ISAT comparable to WT mice and have reduced numbers of Tregs compared with WT B(-/-) mice.


Subject(s)
B-Lymphocytes/immunology , CD28 Antigens/deficiency , T-Lymphocytes, Regulatory/immunology , Thyroiditis, Autoimmune/immunology , Animals , Autoantibodies/immunology , CD4 Antigens/metabolism , Fibrosis/immunology , Forkhead Transcription Factors/metabolism , Inflammation , Interferon-gamma/biosynthesis , Interleukin-6/biosynthesis , Lymphocyte Count , Mice , Mice, Inbred NOD , Mice, Knockout , Receptors, Tumor Necrosis Factor, Type II/metabolism , Sodium Iodide/administration & dosage , Thyroglobulin/immunology , Thyroid Gland/immunology , Thyroiditis, Autoimmune/chemically induced , Thyroxine/blood , Tumor Necrosis Factor Receptor Superfamily, Member 7/metabolism
8.
J Immunol ; 191(3): 1126-35, 2013 Aug 01.
Article in English | MEDLINE | ID: mdl-23817422

ABSTRACT

The events controlling the transition of T cells from effector to memory remain largely undefined. Many models have been put forth to account for the origin of memory precursors, but for CD4 T cells initial studies reported that memory T cells derive from IFN-γ-nonproducing effectors, whereas others suggested that memory emanates from highly activated IFN-γ-producing effectors. In this study, using cell proliferation, expression of activation markers, and production of IFN-γ as a measure of activation, we defined two types of effector CD4 T cells and investigated memory generation. The moderately activated early effectors readily transit to memory, whereas the highly activated late effectors, regardless of their IFN-γ production, develop minimal memory. Boosting with Ag-free adjuvant, however, rescues late effectors from cell death and sustains both survival and IFN-γ cytokine responses in lymphopenic hosts. The adjuvant-mediated memory transition of late effectors involves the function of TLRs, most notably TLR9. These findings uncover the mechanism by which late effector CD4 T cells are driven to transit to memory and suggest that timely boosts with adjuvant may enhance vaccine efficacy.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , Immunologic Memory/immunology , Lymphopenia/immunology , Adjuvants, Immunologic/administration & dosage , Animals , Interferon-gamma , Lymphocyte Activation/immunology , Mice , Mice, Inbred BALB C , Mice, Transgenic , Toll-Like Receptor 9/immunology , Toll-Like Receptor 9/metabolism
9.
Immunology ; 139(2): 179-86, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23293979

ABSTRACT

Approximately 80% of female wild-type non-obese diabetic (WT NOD) mice spontaneously develop diabetes, whereas B-cell-deficient (B(-/-)) NOD mice are resistant to diabetes. B(-/-) mice are also resistant to other spontaneous and experimentally induced autoimmune diseases, including arthritis, systemic lupus erythematosus, Sjögren syndrome and thyroiditis. Under normal conditions, activation of self-reactive T cells in the periphery is limited by CD4(+) CD25(+) natural regulatory T (Treg) cells. B(-/-) NOD.H-2h4 mice, normally resistant to spontaneous autoimmune thyroiditis (SAT), develop SAT when Treg cells are depleted, suggesting that Treg cells are preferentially activated when autoantigen is initially presented by non-B-cell antigen-presenting cells. To test the hypothesis that increased Treg cell activity in B(-/-) mice contributes to their resistance to other autoimmune diseases, WT and B(-/-) NOD mice were given anti-CD25 to transiently deplete CD4(+) CD25(+) Treg cells. The WT and B(-/-) NOD mice given anti-CD25 developed diabetes much earlier than WT mice given rat IgG, whereas rat IgG-treated B(-/-) mice did not develop diabetes. Treg-cell-depleted mice had increased lymphocyte infiltration of the pancreas, salivary glands and thyroid compared with controls given rat IgG. These results are consistent with the hypothesis that resistance of B-cell-deficient NOD mice to several autoimmune diseases is due to the activity of Treg cells.


Subject(s)
Autoimmune Diseases/immunology , B-Lymphocytes/immunology , Lymphocyte Depletion , T-Lymphocytes, Regulatory/immunology , Animals , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal/pharmacology , Antigen-Presenting Cells/immunology , Antigen-Presenting Cells/metabolism , Arthritis/genetics , Arthritis/immunology , Autoantigens/immunology , Autoimmune Diseases/genetics , B-Lymphocytes/metabolism , Diabetes Mellitus, Type 1/genetics , Diabetes Mellitus, Type 1/immunology , Immunoglobulin G/immunology , Immunoglobulin G/pharmacology , Interleukin-2 Receptor alpha Subunit/immunology , Lupus Erythematosus, Systemic/genetics , Lupus Erythematosus, Systemic/immunology , Lymphocyte Activation/immunology , Mice , Mice, Inbred NOD , Rats , Salivary Glands/immunology , Salivary Glands/metabolism , Sjogren's Syndrome/genetics , Sjogren's Syndrome/immunology , T-Lymphocytes, Regulatory/drug effects , T-Lymphocytes, Regulatory/metabolism , Thyroid Gland/immunology , Thyroid Gland/metabolism , Thyroiditis, Autoimmune/genetics , Thyroiditis, Autoimmune/immunology
10.
Int Immunol ; 24(4): 233-42, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22298883

ABSTRACT

B-cell depletion therapy can be effective for treating B-cell lymphomas as well as many human and murine autoimmune diseases. B-cell-deficient mice are normally resistant to spontaneous autoimmune thyroiditis (SAT), but they develop SAT if regulatory T cells are transiently depleted during the first 3-6 weeks after birth. This was also a critical time when B-cell depletion effectively inhibited development of SAT in adult mice. The current study was undertaken to test the hypothesis that transient depletion of B cells using anti-CD20 would be sufficient to suppress SAT if B cells were depleted early in life and that inhibition of SAT would be due to the activity of Treg that functioned most effectively when B cells were absent or low. The results presented here support this hypothesis and indicate that development of autoimmune disease in adults is effectively inhibited when anti-CD20 is administered 1-3 weeks after birth. After 3 weeks, transient B-cell depletion is no longer effective, and B-cell depletion must be maintained to effectively suppress autoimmune disease. B-cell depletion in 1- to 3-week-old mice depletes all B-cell subsets, whereas B-cell depletion initiated in adults spares many marginal zone B cells. Following early B-cell depletion, splenic Treg increase in number, and depletion of Treg reverses the inhibitory effect of anti-CD20 on disease development. Early transient depletion of B cells could be useful for preventing autoimmune disease in individuals at high risk for developing autoimmune diseases as adults.


Subject(s)
B-Lymphocytes/immunology , Lymphocyte Depletion , T-Lymphocytes, Regulatory/immunology , Thyroiditis, Autoimmune/prevention & control , Animals , Antibodies, Monoclonal/administration & dosage , Antibodies, Monoclonal/immunology , Antigens, CD20/immunology , Mice , Mice, Inbred NOD , T-Lymphocytes, Regulatory/metabolism , Thyroiditis, Autoimmune/immunology , Thyroiditis, Autoimmune/pathology
11.
J Immunol ; 187(8): 3979-86, 2011 Oct 15.
Article in English | MEDLINE | ID: mdl-21911603

ABSTRACT

The cell dynamics associated with induction of peripheral T cell tolerance remain largely undefined. In this study, an in vivo model was adapted to two-photon microscopy imaging, and T cell behavior was analyzed on tolerogen-induced modulation. FcγR-deficient (FcγR(-/-)) mice were unable to resist or alleviate experimental allergic encephalomyelitis when treated with Ig-myelin oligodendrocyte glycoprotein (MOG) tolerogen, an Ig carrying the MOG35-55 peptide. However, when FcγR(+/+) dendritic cells (DCs) are adoptively transferred into FcγR(-/-) mice, uptake and presentation of Ig-MOG occurs and the animals were able to overcome experimental allergic encephalomyelitis. We then fluorescently labeled FcγR(+/+) DCs and 2D2 MOG-specific TCR-transgenic T cells, transferred them into FcγR(-/-) mice, administered Ig-MOG, and analyzed both T cell-DC contact events and T cell motility. The results indicate that tolerance takes place in lymphoid organs, and surprisingly, the T cells do not become anergic but instead have a Th2 phenotype. The tolerant Th2 cells displayed reduced motility after tolerogen exposure similar to Th1 cells after immunization. However, the Th2 cells had higher migration speeds and took longer to exhibit changes in motility. Therefore, both Th1 immunity and Th2 tolerance alter T cell migration on Ag recognition, but the kinetics of this effect differ among the subsets.


Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Immune Tolerance/immunology , T-Lymphocyte Subsets/immunology , Adoptive Transfer , Animals , Cell Separation , Chemotaxis, Leukocyte/immunology , Dendritic Cells/immunology , Encephalomyelitis, Autoimmune, Experimental/pathology , Lymphocyte Activation/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Myelin-Associated Glycoprotein/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocyte Subsets/metabolism , T-Lymphocyte Subsets/pathology
12.
J Immunol ; 185(6): 3149-57, 2010 Sep 15.
Article in English | MEDLINE | ID: mdl-20709947

ABSTRACT

The role APCs play in the transition of T cells from effector to memory remains largely undefined. This is likely due to the low frequency at which long-lived T cells arise, which hinders analysis of the events involved in memory development. In this study, we used TCR transgenic T cells to increase the frequency of long-lived T cells and developed a transfer model suitable for defining the contribution of APCs to the development of CD4 T cell memory. Accordingly, naive TCR transgenic T cells were stimulated in vitro with Ag presented by different types of APCs and transferred into MHC class II-deficient mice for parking, and the hosts were later analyzed for long-lived T cell frequency or challenged with suboptimal dose of Ag, and the long-lived cells-driven memory responses were measured. The findings indicate that B cells and CD8alpha(+) dendritic cells sustained elevated frequencies of long-lived T cells that yielded rapid and robust memory responses upon rechallenge with suboptimal dose of Ag. Furthermore, both types of APCs had significant programmed death (PD) ligand 2 expression prior to Ag stimulation, which was maintained at a high level during presentation of Ag to T cells. Blockade of PD ligand 2 interaction with its receptor PD-1 nullified the development of memory responses. These previously unrecognized findings suggest that targeting specific APCs for Ag presentation during vaccination could prove effective against microbial infections.


Subject(s)
Antigen-Presenting Cells/immunology , Antigen-Presenting Cells/metabolism , Antigens, CD/biosynthesis , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , Cell Differentiation/immunology , Immunologic Memory , Intercellular Signaling Peptides and Proteins/biosynthesis , Amino Acid Sequence , Animals , Antigens, CD/genetics , Antigens, CD/metabolism , Antigens, CD/physiology , Apoptosis Regulatory Proteins/genetics , Apoptosis Regulatory Proteins/metabolism , CD4-Positive T-Lymphocytes/cytology , Cell Communication/genetics , Cell Communication/immunology , Cell Differentiation/genetics , Chickens , Histocompatibility Antigens Class II/genetics , Immunologic Memory/genetics , Intercellular Signaling Peptides and Proteins/genetics , Intercellular Signaling Peptides and Proteins/physiology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Transgenic , Molecular Sequence Data , Ovalbumin/immunology , Ovalbumin/metabolism , Peptide Fragments/immunology , Peptide Fragments/metabolism , Programmed Cell Death 1 Ligand 2 Protein , Programmed Cell Death 1 Receptor , Resting Phase, Cell Cycle/genetics , Resting Phase, Cell Cycle/immunology
13.
J Leukoc Biol ; 87(6): 1019-28, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20179154

ABSTRACT

Following activation through the TCR, CD4+ T cells can differentiate into three major subsets: Th1, Th2, and Th17 cells. IL-17-secreting Th17 cells play an important role in the pathogenesis of several autoimmune diseases and in immune responses to pathogens, but little is known about the regulation of apoptosis in Th17 cells. In this study, the sensitivity of in vitro-polarized Th1, Th2, and Th17 cells to Fas-mediated apoptosis was compared directly by different methods. The order of sensitivity of T cell subsets to Fas-mediated apoptosis is: Th1 > Th17 > Th2. The greater sensitivity of Th17 cells to Fas-mediated apoptosis compared with Th2 cells correlated with their higher expression of FasL and comparable expression of the antiapoptotic molecule FLIP. The decreased sensitivity of Th17 compared with Th1 cells correlated with the higher expression of FLIP by Th17 cells. Transgenic overexpression of FLIP in T cells protected all three subsets from Fas-mediated apoptosis. These findings provide new knowledge for understanding how survival of different subsets of T cells is regulated.


Subject(s)
Apoptosis , Interleukin-17/metabolism , Lymphocyte Activation/physiology , Th1 Cells/immunology , Th2 Cells/immunology , fas Receptor/physiology , Animals , Antibodies, Monoclonal/pharmacology , Antibodies, Monoclonal, Murine-Derived , CASP8 and FADD-Like Apoptosis Regulating Protein/metabolism , Caspase 3/metabolism , Cell Differentiation , Cells, Cultured , DNA Primers/chemistry , Enzyme-Linked Immunosorbent Assay , Fas Ligand Protein/metabolism , Female , Flow Cytometry , In Situ Nick-End Labeling , Male , Mice , Mice, Inbred DBA , Mice, Transgenic , Microscopy, Confocal , Reverse Transcriptase Polymerase Chain Reaction , T-Lymphocyte Subsets
14.
J Immunol ; 184(7): 3377-85, 2010 Apr 01.
Article in English | MEDLINE | ID: mdl-20181889

ABSTRACT

Recently, traces of double-positive FoxP3(+)RORgammat(+) T cells were identified and viewed as dual programming differentiation intermediates geared toward development into T regulatory or Th17 cells. In this study, we report that FoxP3(+)RORgammat(+) intermediates arise in the NOD mouse T cell repertoire prior to inflammation and can be expanded with tolerogen without further differentiation. Furthermore, FoxP3(+)RORgammat(+) cells express both CD62L and membrane-bound TGFbeta and use the former to traffic to the pancreas and the latter to suppress effector T cells both in vitro and in vivo. The cells perform these functions as FoxP3(+)RORgammat(+) intermediates, despite being able to terminally differentiate into either FoxP3(+)RORgammat(-) T regulatory or FoxP3(-)RORgammat(+) Th17 cells on polarization. These previously unrecognized observations extend plasticity to both differentiation and function and indicate that the intermediates are poised to traffic to sites of inflammation and target diverse pathogenic T cells, likely without prior conditioning by effector T cells, thus broadening efficacy against autoimmunity.


Subject(s)
Diabetes Mellitus, Type 1/immunology , Forkhead Transcription Factors/immunology , Nuclear Receptor Subfamily 1, Group F, Member 3/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Helper-Inducer/immunology , Animals , Cell Differentiation/immunology , Cell Separation , Diabetes Mellitus, Type 1/metabolism , Flow Cytometry , Forkhead Transcription Factors/metabolism , Interleukin-17/immunology , Interleukin-17/metabolism , Lymphocyte Activation/immunology , Mice , Mice, Inbred NOD , Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism , Reverse Transcriptase Polymerase Chain Reaction , T-Lymphocyte Subsets/cytology , T-Lymphocyte Subsets/metabolism , T-Lymphocytes, Helper-Inducer/cytology , T-Lymphocytes, Helper-Inducer/metabolism
15.
J Exp Med ; 205(10): 2269-80, 2008 Sep 29.
Article in English | MEDLINE | ID: mdl-18762566

ABSTRACT

Primary neonatal T cell responses comprise both T helper (Th) cell subsets, but Th1 cells express high levels of interleukin 13 receptor alpha1 (IL-13R alpha 1), which heterodimerizes with IL-4R alpha. During secondary antigen challenge, Th2-produced IL-4 triggers the apoptosis of Th1 cells via IL-4R alpha/IL-13R alpha 1, thus explaining the Th2 bias in neonates. We show that neonates acquire the ability to overcome the Th2 bias and generate Th1 responses starting 6 d after birth. This transition was caused by the developmental maturation of CD8 alpha(+)CD4(-) dendritic cells (DCs), which were minimal in number during the first few days of birth and produced low levels of IL-12. This lack of IL-12 sustained the expression of IL-13R alpha 1 on Th1 cells. By day 6 after birth, however, a significant number of CD8 alpha(+)CD4(-) DCs accumulated in the spleen and produced IL-12, which triggered the down-regulation of IL-13R alpha 1 expression on Th1 cells, thus protecting them against IL-4-driven apoptosis.


Subject(s)
Animals, Newborn/immunology , Dendritic Cells/immunology , Immunity/immunology , Interleukin-12/biosynthesis , T-Lymphocyte Subsets/immunology , Th2 Cells/immunology , Adoptive Transfer , Animals , Apoptosis/immunology , Cell Survival , Dendritic Cells/cytology , Interferon-gamma/immunology , Interleukin-12/immunology , Interleukin-13 Receptor alpha1 Subunit/genetics , Interleukin-13 Receptor alpha1 Subunit/immunology , Interleukin-4/immunology , Mice , Mice, Inbred BALB C , Mice, SCID , Rabbits , Rats , Spleen/cytology , Spleen/immunology , T-Lymphocyte Subsets/cytology , Th1 Cells/immunology , Th2 Cells/cytology
16.
J Immunol ; 181(1): 73-80, 2008 Jul 01.
Article in English | MEDLINE | ID: mdl-18566371

ABSTRACT

Lately, it has become clear that regulatory T cells (Tregs) play a major role in the maintenance of peripheral tolerance and control of autoimmunity. Despite these critical functions, the process underlying the development of Tregs remains largely undefined. Herein, altered peptide ligand (APL) variants derived from the proteolipid protein-1 (PLP1) epitope were expressed on immunoglobulins (Igs) and the resulting Ig-APLs were used to deliver the APLs from mother to fetus through the maternal placenta to influence thymic T cell selection. This delivery system was then adapted to the SJL/J mouse, a strain that expresses only the DM20 form of PLP, which lacks the dominant PLP1 epitope in the thymus during fetal and neonatal development. This model, which restores thymic T cell selection for PLP1, was then used to determine whether affinity plays a role in the development of Tregs. The findings show that fetal exposure to low-affinity peptide ligand was unable to drive development of Tregs while variants with higher affinity to the TCR resulted in significant seeding of the periphery with mature, naive Tregs. Thus, contrary to pathogenic T cells, Tregs require avid TCR-ligand interaction to undergo thymic development and maturation.


Subject(s)
Cell Differentiation/immunology , Fetus/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes, Regulatory/cytology , T-Lymphocytes, Regulatory/immunology , Animals , Encephalomyelitis, Autoimmune, Experimental/immunology , Immunity, Innate/immunology , Immunoglobulins/immunology , Ligands , Lymphocyte Count , Mice
17.
J Exp Med ; 205(1): 207-18, 2008 Jan 21.
Article in English | MEDLINE | ID: mdl-18195074

ABSTRACT

The role of Th17 cells in type I diabetes (TID) remains largely unknown. Glutamic acid decarboxylase (GAD) sequence 206-220 (designated GAD2) represents a late-stage epitope, but GAD2-specific T cell receptor transgenic T cells producing interferon gamma (IFNgamma) protect against passive TID. Because IFNgamma is known to inhibit Th17 cells, effective presentation of GAD2 peptide under noninflammatory conditions may protect against TID at advanced disease stages. To test this premise, GAD2 was genetically incorporated into an immunoglobulin (Ig) molecule to magnify tolerance, and the resulting Ig-GAD2 was tested against TID at different stages of the disease. The findings indicated that Ig-GAD2 could not prevent TID at the preinsulitis phase, but delayed TID at the insulitis stage. More importantly, Ig-GAD2 sustained both clearance of pancreatic cell infiltration and beta-cell division and restored normoglycemia when given to hyperglycemic mice at the prediabetic stage. This was dependent on the induction of splenic IFNgamma that inhibited interleukin (IL)-17 production. In fact, neutralization of IFNgamma led to a significant increase in the frequency of Th17 cells, and the treatment became nonprotective. Thus, IFNgamma induced by an adjuvant free antigen, contrary to its usual inflammatory function, restores normoglycemia, most likely by localized bystander suppression of pathogenic IL-17-producing cells.


Subject(s)
Antigens/chemistry , Blood Glucose/metabolism , Gene Expression Regulation , Hyperglycemia/genetics , Interferon-gamma/metabolism , Interleukin-17/antagonists & inhibitors , Animals , Epitopes/chemistry , Hyperglycemia/prevention & control , Interleukin-17/metabolism , Islets of Langerhans/metabolism , Mice , Mice, Inbred NOD , Mice, SCID , Models, Biological , Phenotype , Recombinant Proteins
18.
J Immunol ; 180(3): 1508-16, 2008 Feb 01.
Article in English | MEDLINE | ID: mdl-18209046

ABSTRACT

A number of Ag-specific approaches have been developed that ameliorate experimental allergic encephalomyelitis (EAE), an animal model for the human autoimmune disease multiple sclerosis. Translation to humans, however, remains a consideration, justifying the search for more insight into the mechanism underlying restoration of self-tolerance. Ig-proteolipid protein (PLP) 1 and Ig-myelin oligodendrocyte glycoprotein (MOG) are Ig chimeras carrying the encephalitogenic PLP 139-151 and MOG 35-55 amino acid sequence, respectively. Ig-PLP1 ameliorates EAE in SJL/J (H-2(s)) mice while Ig-MOG modulates the disease in C57BL/6 (H-2(b)) animals. In this study, we asked whether the chimeras would suppress EAE in F(1) mice expressing both parental MHC alleles and representing a polymorphism with more relevance to human circumstances. The results show that Ig-MOG modulates both PLP1 and MOG peptide-induced EAE in the F(1) mice, whereas Ig-PLP1 counters PLP1 EAE but exacerbates MOG-induced disease. This in trans aggravation of MOG EAE by Ig-PLP1 operates through induction of PLP1-specific T cells producing IL-5 that sustained inhibition of MOG-specific Abs leading to exacerbation of EAE. Thus, in trans T cell tolerance, which should be operative in polymorphic systems, can aggravate rather than ameliorate autoimmunity. This phenomenon possibly takes place through interference with protective humoral immunity.


Subject(s)
Autoantibodies/immunology , Chimera/immunology , Encephalomyelitis, Autoimmune, Experimental/immunology , Immune Tolerance , T-Lymphocytes/immunology , Animals , Chimera/genetics , Encephalomyelitis, Autoimmune, Experimental/genetics , Epitopes/immunology , Glycoproteins/genetics , Glycoproteins/immunology , Humans , Immunoglobulins/genetics , Interleukin-5/metabolism , Mice , Mice, Mutant Strains , Myelin Proteins , Myelin Proteolipid Protein/genetics , Myelin Proteolipid Protein/immunology , Myelin-Associated Glycoprotein/genetics , Myelin-Associated Glycoprotein/immunology , Myelin-Oligodendrocyte Glycoprotein , Nerve Tissue Proteins/immunology , Peptide Fragments/genetics , Peptide Fragments/immunology , Peptides/immunology
19.
J Immunol ; 180(1): 179-87, 2008 Jan 01.
Article in English | MEDLINE | ID: mdl-18097018

ABSTRACT

Currently, transition of T cells from effector to memory is believed to occur as a consequence of exposure to residual suboptimal Ag found in lymphoid tissues at the waning end of the effector phase and microbial clearance. This led to the interpretation that memory arises from slightly activated late effectors producing reduced amounts of IFN-gamma. In this study, we show that CD4 T cells from the early stage of the effector phase in which both the Ag and activation are optimal also transit to memory. Moreover, early effector T cells that have undergone four divisions expressed significant IL-7R, produced IFN-gamma, and yielded rapid and robust memory responses. Cells that divided three times that had marginal IL-7R expression and no IFN-gamma raised base level homeostatic memory, whereas those that have undergone only two divisions and produced IFN-gamma yielded conditioned memory despite low IL-7R expression. Thus, highly activated early effectors generated under short exposure to optimal Ag in vivo develop into memory, and such transition is dependent on a significant production of the cell's signature cytokine, IFN-gamma.


Subject(s)
Immunologic Memory , Interferon-gamma/metabolism , T-Lymphocytes/immunology , Animals , Cell Division , DNA-Binding Proteins/genetics , Interferon-gamma/genetics , Lymphocyte Activation , Mice , Mice, Inbred Strains , Mice, Transgenic , Receptors, Interleukin-7/genetics , Receptors, Interleukin-7/metabolism , Spleen/immunology
20.
J Immunol ; 174(11): 6772-80, 2005 Jun 01.
Article in English | MEDLINE | ID: mdl-15905518

ABSTRACT

To date, very few Ag-based regimens have been defined that could expand T regulatory (Treg) cells to reverse autoimmunity. Additional understanding of Treg function with respect to specificity and broad suppression should help overcome these limitations. Ig-proteolipid protein (PLP)1, an Ig carrying a PLP1 peptide corresponding to amino acid residues 139-151 of PLP, displayed potent tolerogenic functions and proved effective against experimental allergic encephalomyelitis (EAE). In this study, we took advantage of the Ig-PLP1 system and the PLP1-specific TCR transgenic 5B6 mouse to define a regimen that could expand Ag-specific Treg cells in vivo and tested for effectiveness against autoimmunity involving diverse T cell specificities. The findings indicate that in vivo exposure to aggregated Ig-PLP1 drives PLP1-specific 5B6 TCR transgenic cells to evolve as Treg cells expressing CD25, CTLA-4, and Foxp3 and producing IL-10. These Treg cells were able to suppress PLP1 peptide-induced EAE in both SJL/J and F(1) (SJL/J x C57BL/6) mice. However, despite being effective against disease induced with a CNS homogenate, the Treg cells were unable to counter EAE induced by a myelin basic protein or a myelin oligodendrocyte glycoprotein peptide. Nevertheless, activation with Ag before transfer into the host mice supports suppression of both myelin oligodendrocyte glycoprotein- and myelin basic protein peptide-induced EAE. Thus, it is suggested that activation of Treg cells by the cognate autoantigen is necessary for operation of broad suppressive functions.


Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Encephalomyelitis, Autoimmune, Experimental/prevention & control , Lymphocyte Activation/immunology , Myelin Proteolipid Protein/immunology , Peptide Fragments/immunology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/metabolism , Amino Acid Sequence , Animals , Bystander Effect/genetics , Bystander Effect/immunology , Cell Proliferation , Encephalomyelitis, Autoimmune, Experimental/pathology , Epitopes, T-Lymphocyte/administration & dosage , Epitopes, T-Lymphocyte/genetics , Epitopes, T-Lymphocyte/immunology , Female , Lymphocyte Activation/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Molecular Sequence Data , Myelin Proteolipid Protein/administration & dosage , Myelin Proteolipid Protein/genetics , Peptide Fragments/administration & dosage , Peptide Fragments/genetics , Rats , Receptors, Antigen, T-Cell/genetics , Recurrence , Severity of Illness Index , T-Lymphocytes, Regulatory/cytology , T-Lymphocytes, Regulatory/transplantation
SELECTION OF CITATIONS
SEARCH DETAIL
...