[Effects of arginine, administered orally, on the endogenous secretion of the STH-somatomedin complex in young human volunteers]. / Effets de l'arginine, administrée par voie orale, sur la sécrétion endogène du complexe STH-somatomédines, chez l'homme volontaire à jeun.

Arginine, administered orally (arginine aspartate 37.5 g), induces upon voluntary fasting man laying down in bed (muscular rest), an increase of: 1) plasmatic HGH rate (radioimmunological) at 2 hours, 2) plasmatic SMs rate (biological: incorporation of 3H thymidine in human lymphocytes) at 8 hours, when compared with an equal administration of aminoacids without arginine or aspartate as placebo. Arginine enhances endogenous secretion of the complex HGH-somatomedines.

[Biphasic action of human growth hormones, of hypophyseal origin or bacterial production, on the lipolysis of rat adipocytes in vitro]. / Action biphasique des hormones de croissance humaines, d'origine hypophysaire ou de fabrication bactérienne, sur la lipolyse d'adipocyte de rat "in vitro."

Actions of: human growth hormone resultant from hypophysis: HGH h (contaminated by beta LPH 1%) or produced by bacteria HGH b (pure), beta LPH resultant from human hypophysis, one of the somatomedins: multiplication stimulating activity (MSA), produced from a culture of rat hepatocytes, are considered in lipolysis of rat adipocytes "in vitro": basal, stimulated by epinephrine (0.1 and 1 microgram/ml), and when insulin is present (0.1 and 1 mUI/ml), during two periods I: 0-1 h 30, II: 1 h 30 - 4 h (hormonal addition at time 0). MSA appears to be antilipolytic during the two periods, between 0 and 4 h, with 500 ng/ml. A dose of 200 ng/ml is inactive. Beta LPH is lipolytic: 400 and 230 ng/ml, during phase I. At the ratio of the contaminate of HGH h 1000 ng/ml, resultant from hypophysis: 10 ng/ml, the action is less significant. Human growth hormones HGH h and HGH b appear to be: antilipolytic during the first period 0 1 h 30 with 1000 and 300 ng/ml, and, then lipolytic between 1 h 30 and 4 h with 1000, 300 and 100 ng/ml. The effect is pure with HGH b during the two periods, and, principally, during phase I when there is an absence of lipolytic contaminate. Biphasic action: antilipolytic, lipolytic, ascertained with a pure growth hormone: HGH b "in vitro" represents an effect "per se", non mediated by an eventual "in vivo" action of somatomedins.

[Effects of insulin, somatomedin--a multiplication stimulating activity (MSA)--and transferrin, on the lipolysis of rat adipocytes in vitro]. / Effects de l'insuline, d'une somatomédine--facteur de multiplication cellulaire (MSA)--et de la transferrine, sur la lipolyse de l'adipocyte de rat in vitro.

Antilipolytic effect was researched when insulin (0.1 and 1 mIU/ml), MSA (200 and 500 ng/ml) and transferrin (2 and 5 micrograms/ml) were added to a suspension of freshly isolated rat adipocytes in vitro. Lipolysis was measured as glycerol secretion in the medium: micromoles/90 minutes/100 mg total lipids. Insulin (1 mIU/ml) reduced adrenalinic stimulation of lipolysis: A 1 microgram/ml (P less than 0.05). MSA 200 ng/ml had no effect. MSA 500 ng/ml reduced basal lipolysis and adrenalinic stimulation (P less than 0.05), and increased insulin-induced antilipolysis (P less than 0.05). Transferrin was active, only when insulin is present: antilipolysis increased (P less than 0.05).

[Effects of arginine aspartate when administered orally, upon nitrogen balance of the rabbit, normal or treated by glucocorticoids (author's transl)]. / Effets de l'aspartate d'arginine, administré par voie orale, sur le bilan d'azote du lapin normal ou traité par l'hydrocortisone.

A nitrogen balance study is performed upon young male rabbit, non treated or treated with glucocorticoids (hydrocortisone : 15 mg a day for 18 days). Arginine aspartate, when administered daily "per os" at fast, 1 500 mg/kg for 4 days, increases strongly nitrogen retention, in non treated rabbit (p less than 0,01). During the last four days of the glucocorticoids treatment, the product (1 500 mg/kg a day) corrects the decrease (p less than 0,01) of nitrogen balance induced by hydrocortisone. Nitrogen retention appears to be more important (p less than 0,01) than the one induced by hydrocortisone alone, and returns to the non treated rabbit value. Arginine aspartate oral administration can provoke, by the way of stimulation of endogenous growth hormone secretion, a protein anticatabolic effect.

[Effects of clofibride upon lipolysis from normal rat adipocytes in vitro (author's transl)]. / Effets du clofibride sur la lipolyse d'adipocytes de rat normal in vitro.

One dose of clofibride (Cf) is administered, after sacrifice of normal fasting rat, in vitro: 2 mg/100 mg adipocytes in 2 ml medium. Lipolysis is studied by the way of glycerol release: micromoles/90 minutes/100 mg total lipids, without or with hormones: epinephrine (10 micrograms/ml), insulin (10(-3) U.I./ml), or association of both. 1. In vitro adjunction of clofibride alone, reduces basal lipolysis (p less than 0,05). 2. Clofibride strongly limits the effects of lipolysis stimulation induced by epinephrine (p less than 0,01). 3. However, it does not increase the discrete antilipolytic effect of insulin, and does not reduce significantly lipolysis induced by the epinephrine + insulin association. Clofibride hypolipaemic effects could be due, partially, to a reduction of hormono-dependent lipolysis.

[Potentiation by clofibride of the heparin-induced clearing reaction (intravascular hydrolysis of neutral lipids), in hyperlipemic rabbits]. / Potentialisation par le clofibride de la réaction de clarification à l'héparine (hydrolyse intravasculaire des lipides neutres), chez le lapin hyperlipémique.

A 16 days clofibride treatment in hyperlipaemic rabbit (fat supply), reduces hypertriglyceridemia and hypercholesterolemia. Heparine clearing reaction, performed on the 17th day, with the daily clofibride dose ingested in one time, is increased. The exaggerated intravascular lipids hydrolysis could explain, in part, the hypolipaemic action of clofibride.

[Effect of clofibride on the lipolysis of perirenal adipose tissue in hyperlipemic rabbits]. / Actions du clofibride sur la lipolyse du tissu adipeux périrénal chez le lapin hyperlipémique.

In hyperlipaemic rabbits (fat supply), perirenal adipose tissue lipolysis does not vary with one in vitro clofibride dose. After a 16 days pretreatment, one in vitro clofibride dose reduces the basal lipolysis and the lipolytic action of synacthene, and increases the antilipolytic effect of insulin. The reduction of the lipolysis of adipose tissue could explain, in part, the hypolipaemic action of clofibride.

[Oral administration effects, with different doses, of arginine aspartate and arginine chlorhydrate upon growth hormone and free fatty acids plasmatic rates in normal fasting children (author's transl)]. / Effets de l'administration "Per Os", à doses variables, d'aspartate d'arginine et de chlorhydrate d'arginine sur les taux d'hormone de croissance et d'acides gras libres du plasma chez l'enfant normal à jeun.

With an equivalent arginine base furniture, arginine chlorhydrate (CA) and arginine aspartate (AA) are orally administered, with variable doses, to normal fasting children, to study during 4 hours evolution of plasmatic growth hormone rates (stimulation of endogenous GH secretion) and plasmatic free fatty acids rates (secondary effects on effectors). With CA 5 g (half dose) opr 10 g (total dose, about 500 mg/kg) mean plasmatic GH rates increase is etale and non significant, when compared to normals. With AA 7,35 g (half dose) or 14,70 g (total dose), there is a 2 h GH peak, when compared to initial values and 2 h normal values (p less than 0,05 or p less than 0.01). Area increases with the two doses AA, when compared to normals (p less than 0,01). With the two products, mean 1 h plasmatic FFA rate does not vary or non significantly decreases. 4 h rate increases, when compared to normals, with CA and AA half dose (p less than 0,05) and CA and AA total dose (p less than 0,01).

[Arginine aspartate effects on acute human alcoholizing test (author's transl)]. / Effets de l'aspartate d'arginine sur l'épreuve d'alcoolisation aiguë sur l'homme.

One oral arginine aspartate administration (10g), 30 minutes after the beginning of acute human alcoholizing test (150 ml whisky 45 degrees, 67,5 g pure alcohol, mean load about 1 g/kg), provokes the following effects. Clinically, intensity and lasting alcohol manifestations are reduced. Alcoholemic absolute curve (g/l) does not vary: 1 h peak equal in the two treated and non treated groups, similar 6 h levels but a little lesser in the treated subjects, without significative difference. Alcohol space does not vary. Arginine aspartate provokes a significant increase between 1 h and 4 h of alcoholemic disparition slope, when rapported to body weight: mg/kg/h (p < 0,02), and ethyloxydation coefficient: mg/kg/h (p < 0,05).

[Arginine aspartate effects on rabbit acute and chronic alcoholization (author's transl)]. / Effets de l'aspartate d'arginine sur l'alcoolisation aiguë et chronique du lapin.

Oral arginine aspartate treatment effects (acute administration: 1 g 30 minutes after load, chronic administration: 0.33 g a day during 9 months) are researched on rabbit acute alcoholizing load (1 ml alcohol 40 degrees/100 g) and alcohol chronic intoxication (0,5 ml alcohol 40 degrees/100 g a day during 9 months). 1) Arginine aspartate acute administration decreases 6 h alcoholemic rates, when compared to normals T + t receiving an equal nutritional placebo at 30 minutes (p < 0.01), without 1 h peak modification, and increases ethyloxydation coefficient (p < 0,01). Aspartate, arginine or pyruvate isolated administration at 30 minutes, increases ethyloxydation coefficient in following order: arginine (no significant difference with T + t), pyruvate + arginine and pyruvate (limit p 0,10 or p < 0,05), aspartate (p < 0,05). It is maximum with arginine aspartate (p < 0.01). 2) Arginine aspartate chronic administration partially reduces hyperalcoholemy (p < 0,01) and hypertriglyceridemy (p < 0.10), strongly increased in non treated alcoholized (p < 0.01). Transaminases rates, which remained about normal in non treated alcoholized, decrease under same time alcoholized (p < 0,10) and 0 values (p < 0.05). Hepatic histology shows, after 9 months, in alcoholized group, inflammatory oedema with some cellular damage, without steatosis. Arginine aspartate seems to provoke some hepatic protection with cellular regeneration.

[Evoluation of biologic balance as a function of radiologic status in a population living in a endemic fluoride zone of southern Algeria]. / Evolution du bilan biologique en fonction du stade radiologique chez une population vivant dans une zone d'endémie fluorée du sud algérien

Chronic fluoride intoxication in subjects living in an endemic South Algerian zone: El Oued (drinking water fluor: 3 to 5 mg/l), provokes some blood and urinary levels modifications, when compared to normals living in Algiers (drinking water fluor: 0,6 mg/l). These modifications are the more often present in stade 0 (normal radiologic aspect) and do not increase with radiological evolution (stades I, II, III). Fluoremy and fluorury increase. Phosphocalcic metabolism is altered. Tubular reabsorption coefficient, particularly, decreases strongly. Using renal functional exploration, a pretty soon tubular failure is founded, which preceeds glomerular failure. Blood levels of certain products and enzymes are studied.

[Effects of arginine and growth hormone, administered in vivo and in vitro, on the oxygen consumption of rat liver hemogenates]. / Effets de l'arginine et de l'hormone croissance administrées "in vivo" et "in vitro" sur la consommation d'oxygène d'homogénats de foie de rat

Effects of arginine aspartate, arginine chlorhydrate, potassium aspartate, with equal arginine base and aspartate ion available for all there compounds, growth hormone, and association of these different substances, are studied on rat hepatic homogenates oxygen consumption during 7 to 12 h, after injection to rats 1 h before sacrifice (A.A.: 0.50 g, C.A.: 0.34 g, A.K.: 0.22 g, STH: 2 U.I.), or "in vitro" adjunction to the preparation (A.A.: 0.125 g, C.A.: 0.085 g. A.K.: 0.055 g. STH: 0.5 U.I./0.25 g of liver). 1) "In vivo", arginine (endogenous STH secretion stimulation) is active: A.A. (+23% to +96% from 1 h to 8 h, p is less than 0.01), C.A. (+49% from 5 h to 8 h, p is less than 0.01), whereas potassium aspartate is inactive. "In vitro", aspartate is active: A.K. (+24% to +147% from 4 h to 12 h, (p is less than 0.01), A.A. (+56% to +53% from 7 h to 12 h, p is less than 0.01), whereas arginine without aspartate: C.A. is inactive. 2) Exogenous STH is active "in vivo" (+111% from 5 h to 8 h, p is less than 0.01) and "in vitro" (+31% from 4 h to 7 h, p is less than 0.01). 3) Association A.A. + STH (endogenous STH + exogenous STH) when given "in vivo", dose not produce any additive effect the tissue Vo2 release (+91% from 5 h to 8 h, p is less than 0.01) does not differ from STH and A.A. Aspartate added to STH "in vitro" enhances STH effects: (A.A. + STH: + 44% to +114% from 4 h to 12 h, p is less than 0.01, A.K. + STH: 73% to + 152% from 4 h to 12 h, p is less than 0.01).

[Effect of subacute fluorine poisoning in the rabbit on fluorine and phosphorus-calcium metabolism and on radiography of the skeleton]. / Effets de l'intoxication fluorée subaiguë du lapin sur les métabolismes fluoré et phosphocalcique et sur la radiographie du squelette.

Rabbit subacute fluoride intoxication effects (21,4 mg a day for 10 months) are researched on fluor, calcium and phosphorus metabolism and on skeletal radiology. Fluoremy increase (p less than 0.05) and there is a strong fluor retention (p less than 0.01), due to fluor digestive utilization coefficient increase (p less than 0.05), in spite of relative hyperfluorury (p less than 0.05). Calcemy decreases (p less than 0.01), but phosphatemy and alkaline phosphatasemy do not vary but a little. Calcium and phosphorus balances become negative (p less than 0.05), due to calcium digestive utilization coefficient inversion (p less than 0.05) and phosphorus digestive utilization coefficient decrease (p less than 0.05) and hypercalciury (p less than 0.05) and hyperphosphatury (p less than 0.05) with phosphorus renal reabsorption coefficient decrease (p less than 0.05). Some skeletal radiological abnormalities appear on rachis and limbs.

[The effects of arginine and growth hormone on the nitrogen balance of rats]. / Effets de l'arginine et de l'hormone de croissance sur le bilan d'azote du rat

Intramuscular administration effects of arginine aspartate (AA : 0,50 g a day for 4 days), arginine chlorhydrate (CA : 0.34 g a day for 4 days), potassium aspartate (AK : 0.22 g a day for 4 days) with equal arginine base and aspartate ion supply for all products, bovine growth hormone (STH : 2 U.I. a day for 4 days), and the association of these substances, are studied on 6 rats, during a 74 days nitrogen balance period. Arginine (endogenous STH secretion stimulation) produces a greater nitrogen retention with AA (+ 184%, p less than 0.01) than CA (+ 71% p less than 0.10). A.K. had no effect. With exogenous STH, effect is important (+ 248%, p less than 0.01) but does not significantly differ from AA. STH + A.A. and STH + C.A. associations (exogenous and endogenous STH) produce a very marked nitrogen retention (+ 282% and + 252%, p less than 0.01) which, however, does not significantly differ from STH when given alone.