Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Exp Parasitol ; 182: 34-36, 2017 Nov.
Article in English | MEDLINE | ID: mdl-28935536

ABSTRACT

Metronidazole is administered in an inactive form then activated to its cytotoxic form within the hydrogenosome of trichomonads. Two hydrogenosomal proteins, pyruvate ferredoxin oxidoreductase (PFOR) and ferredoxin, play a critical role in the reductive activation of metronidazole. The expression of these proteins and other hydrogenosomal proteins are likewise positively regulated by iron. In the present study, the effect of iron on minimal lethal concentration (MLC) of metronidazole on in vitro cultured Trichomonas vaginalis(T. vaginalis) isolates was investigated. Interestingly, Addition of Ferrous ammonium sulphate (FAS) to T. vaginalis culture led to decrease in the MLC of metronidazole. On using aerobic assay, MLC of metronidazole on untreated T. vaginalis of both isolates was 12.5 µg/ml that decreased to 0.38 µg/ml on FAS treated trichomonads. Also anaerobic assay revealed that MLC on untreated parasites was 3.12 µg/ml that decreased to 0.097 µg/ml and 0.19 µg/ml for isolate 1 and isolate 2 respectively after iron addition. It was concluded that, addition of iron to in vitro cultured T. vaginalis decreases metronidazole MLC that was detected by both aerobic and anaerobic assays.


Subject(s)
Antiprotozoal Agents/pharmacology , Iron/pharmacology , Metronidazole/pharmacology , Trichomonas Vaginitis/parasitology , Trichomonas vaginalis/drug effects , Vaginal Discharge/parasitology , Adult , Aerobiosis , Anaerobiosis , Antiprotozoal Agents/metabolism , Drug Interactions , Female , Ferredoxins/metabolism , Ferrous Compounds/pharmacology , Humans , Lethal Dose 50 , Metronidazole/metabolism , Oxidation-Reduction , Pyruvate Synthase/metabolism , Quaternary Ammonium Compounds/pharmacology
2.
Exp Parasitol ; 134(4): 474-81, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23684568

ABSTRACT

The interactions between various aspects of the immune responses mediated by concomitant parasite infections may influence the resultant cytokines profiles. We tested this hypothesis by developing two Schistosoma mansoni and Echinococcus granulosus coinfection murine models. Our aim was to explore the effect of echinoccocis on the immune responses induced by schistosomiasis, either when the two infections were induced synchronously or when echinococcosis was induced during egg deposition period of S. mansoni infection. The proliferation of antigens specific stimulated splenocytes, taken from studied groups, was determined. Then, IFN-γ, and IL-10 production from stimulated cells were measured. Significant elevation of IFN-γ, 4weeks after synchronous coinfection, was occurred compared to S. mansoni infected group, associated with modest elevation of IL10 level. On the other hand, echinococcosis coinfection during egg deposition period of schistosomiasis resulted in significant marked reduction in IL10 level in comparison to S. mansoni infected mice. These results suggested that echinococcosis coinfection, during the switching from Th1 to Th2 cytokine stage of murine schistosomiasis, can alter the ability of S. mansoni infection to skew the cytokines response towards Th2 profile. It is clear that the timing and sequence of concomitant infections are of vital importance for the outcome of the immune response.


Subject(s)
Echinococcosis/complications , Echinococcus granulosus/immunology , Interferon-gamma/metabolism , Interleukin-10/metabolism , Schistosomiasis mansoni/complications , Animals , Biomphalaria , Camelus , Cell Proliferation , Coinfection , Echinococcosis/immunology , Female , Mice , Random Allocation , Schistosomiasis mansoni/immunology , Spleen/cytology , Spleen/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...